Generation of cytokines in red cell concentrates during storage is prevented by prestorage white cell reduction

Shanwell, Agneta; Kristiansson, Marianne; Remberger, Mats; Ringdén, Olle

doi:10.1046/j.1537-2995.1997.37797369441.x

Cited by 158 publications

(132 citation statements)

References 35 publications

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“…Consistent with this finding and with cytokine assays in units of uncontaminated, leukodepleted RBCs (33), levels of the proinflammatory cytokines IL-6, IL-8 (SI Fig. 8), IL-1␤, and tumor necrosis factor (TNF)-␣ (data not shown) in all samples were extremely low and did not rise during storage.…”

Section: Resultssupporting

confidence: 79%

Evolution of adverse changes in stored RBCs

Bennett‐Guerrero¹,

Veldman²,

Doctor

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

539

556

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Recent studies have underscored questions about the balance of risk and benefit of RBC transfusion. A better understanding of the nature and timing of molecular and functional changes in stored RBCs may provide strategies to improve the balance of benefit and risk of RBC transfusion. We analyzed changes occurring during RBC storage focusing on RBC deformability, RBC-dependent vasoregulatory function, and S-nitrosohemoglobin (SNO-Hb), through which hemoglobin (Hb) O2 desaturation is coupled to regional increases in blood flow in vivo (hypoxic vasodilation). Five hundred ml of blood from each of 15 healthy volunteers was processed into leukofiltered, additive solution 3-exposed RBCs and stored at 1-6°C according to AABB standards. Blood was subjected to 26 assays at 0, 3, 8, 24 and 96 h, and at 1, 2, 3, 4, and 6 weeks. RBC SNO-Hb decreased rapidly (1.2 ؋ 10 ؊4 at 3 h vs. 6.5 ؋ 10 ؊4 (fresh) mol S-nitrosothiol (SNO)/mol Hb tetramer (P ‫؍‬ 0.032, mercuric-displaced photolysis-chemiluminescence assay), and remained low over the 42-day period. The decline was corroborated by using the carbon monoxide-saturated copper-cysteine assay [3.0 ؋ 10 ؊5 at 3 h vs. 9.0 ؋ 10 ؊5 (fresh) mol SNO/mol Hb]. In parallel, vasodilation by stored RBCs was significantly depressed. RBC deformability assayed at a physiological shear stress decreased gradually over the 42-day period (P < 0.001). Time courses vary for several storage-induced defects that might account for recent observations linking blood transfusion with adverse outcomes. Of clinical concern is that SNO levels, and their physiological correlate, RBC-dependent vasodilation, become depressed soon after collection, suggesting that even ''fresh'' blood may have developed adverse biological characteristics.adenosine triphosphate ͉ hemoglobin ͉ nitric oxide ͉ S-nitrosothiols ͉ transfusion

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Section: Resultssupporting

confidence: 79%

Evolution of adverse changes in stored RBCs

Bennett‐Guerrero¹,

Veldman²,

Doctor

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

539

556

View full text Add to dashboard Cite

show abstract

“…First, investigators have found that prestorage centrifugation or filtration of red blood cell units removes 99.995% of leukocytes, 35 reducing the concentration of bioactive lipids and inflammatory cytokines. [36][37][38] To attenuate the potential harmful effects of red blood cell transfusion, universal pre-storage leukoreduction was introduced in Canada in July 1999. However, despite considerable theoretical advantages, clinical benefits of leukoreduction have been inconsistent in observational studies [39][40][41] and randomized trials.…”

Section: Discussionmentioning

confidence: 99%

Early massive transfusion in trauma patients: Canadian single-centre retrospective cohort study

Mahambrey

Fowler

Pinto

et al. 2009

Can J Anesth/J Can Anesth

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Purpose To determine associations between red blood cell (RBC) transfusion and early and late clinical outcomes in massively transfused adult trauma patients. Methods A retrospective cohort study (1992)(1993)(1994)(1995)(1996)(1997)(1998)(1999)(2000)(2001) including 260 patients receiving C10 RBC units B24 hr after admission to a university-affiliated trauma centre. We extracted demographic and clinical data and used multivariable regression to determine independent effects of RBC transfusion on clinical outcomes. ]).Conclusions Hospital mortality is high among massively transfused trauma patients. Among early survivors, 48-hr RBC transfusion volume is associated with increased organ dysfunction, but not hospital mortality. Also, it is not associated with 48-hr mortality. Future research should continue to explore methods to improve hemostasis and minimize the need for RBC transfusion. RésuméObjectif De´terminer les associations qui existent entre la transfusion d'e´rythrocytes et les devenirs cliniques R. A. Fowler and N. K. J. Adhikari contributed equally to this study.

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“…Both leucocyte-derived and platelet-derived chemokines progressively accumulate in RBC during storage [82][83][84][85]. Pre-storage leucocyte reduction eliminates chemokine accumulation in RBCs [82,83,85].…”

Section: Accumulation Of Chemokines During Blood Component Storagementioning

confidence: 99%

An introduction to chemokines and their roles in transfusion medicine

Davenport

2009

Vox Sanguinis

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Chemokines are a set of structurally related peptides that were first characterized as chemoattractants and have subsequently been shown to have many functions in homeostasis and pathophysiology. Diversity and redundancy of chemokine function is imparted by both selectivity and overlap in the specificity of chemokine receptors for their ligands. Chemokines have roles impacting transfusion medicine in haematopoiesis, haematologic malignancies, transfusion reactions, graft-versus-host disease, and viral infections. In haematopoietic cell transplantation, chemokines are active in mobilization and homing of progenitor cells, as well as mediating T-cell recruitment in graft-versus-host disease. Platelets are rich source of chemokines that recruit and activate leucocytes during thrombosis. Important transfusion-transmissible viruses such as cytomegalovirus and human immunodeficiency virus exploit chemokine receptors to evade host immunity. Chemokines may also have roles in the pathophysiology of haemolytic and non-haemolytic transfusion reactions.

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Generation of cytokines in red cell concentrates during storage is prevented by prestorage white cell reduction

Abstract: Prestorage filtration seems to diminish the amount of IL-1, IL-6, IL-8, and TNF alpha RBCs during storage. The possible clinical implications of this should be elucidated.

Cited by 158 publications

References 35 publications

Evolution of adverse changes in stored RBCs

Evolution of adverse changes in stored RBCs

Early massive transfusion in trauma patients: Canadian single-centre retrospective cohort study

An introduction to chemokines and their roles in transfusion medicine

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