Generation of contaminating fibrin during storage of SAGM-suspended red cells

Skjensberg, O.H.; Kierulf, Peter; Godal, H. C.

doi:10.1016/0049-3848(86)90285-9

“…The clinical significance of such changes to transfusion practice is uncertain but might be avoided, if it is found desirable, by use of an additive solution containing extra anticoagulant (heparin or citrate) after plasma harvesting. Such an approach has been suggested by others [38,40] for similar reasons and also to reduce the extent of macroaggregate formation [42], and would correspond in terms of pack and pro- Smit-Sibinga and Das [5].…”

Section: Discussionmentioning

confidence: 95%

Studies on the Procurement of Blood Coagulation Factor VIII in vitro Studies on Blood Components Prepared in Half‐Strength Citrate Anticoagulant

Prowse

¹

,

Waterston

²

,

Dawes

³

et al. 1987

View full text Add to dashboard Cite

The effect of replacing a standard citrate anticoagulant with one containing half the amount of citrate on the in vitro properties of components prepared from blood donations was investigated. This resulted in a significant improvement in factor VIII stability such that there was little loss during overnight storage, and this was reflected in the factor VIII yield in cryoprecipitate. The quality of cellular components in red cell units stored up to 35 days or platelet concentrates stored up to 7 days was not adversely affected. Although initial levels were similar to those in standard anticoagulant, the extent of fibrinopeptide A generation and complement C3 breakdown in red cell units stored for 35 days in half-strength citrate was somewhat increased.

show abstract

“…Similar, but less apparent changes in these parameters have been noted by others for donations taken in standard anticoagulants [37][38][39][40][41], The clinical signifi cance of such changes to transfusion practice is uncertain but might be avoided, if it is found desirable, by use of an additive solution containing extra anticoagulant (heparin or citrate) after plasma harvesting. Such an approach has been suggested by others [38,40] for similar reasons and also to reduce the extent of macroaggregate formation [42], and would correspond in terms of pack and pro cessing requirements to that of Rock et al [4,6] and Smit-Sibinga and Das [5].…”

Section: Platelet Concentratesmentioning

confidence: 86%

Studies on the Procurement of Blood Coagulation Factor VIII in vitro Studies on Blood Components Prepared in Half-Strength Citrate Anticoagulant

Prowse¹,

Waterston²,

Dawes³

et al. 1987

View full text Add to dashboard Cite

The effect of replacing a standard citrate anticoagulant with one containing half the amount of citrate on the in vitro properties of components prepared from blood donations was investigated. This resulted in a significant improvement in factor VIII stability such that there was little loss during overnight storage, and this was reflected in the factor VIII yield in cryoprecipitate. The quality of cellular components in red cell units stored up to 35 days or platelet concentrates stored up to 7 days was not adversely affected. Although initial levels were similar to those in standard anticoagulant, the extent of fibrinopeptide A generation and complement C3 breakdown in red cell units stored for 35 days in half-strength citrate was somewhat increased.

show abstract