Generation of cell-derived matrices that support human NK cell migration and differentiation

Lee, Barclay J.; Hegewisch-Solloa, Everardo; Shannon, Michael J.; Mace, Emily M.

doi:10.1002/jlb.1ma0420-635r

Cited by 12 publications

(14 citation statements)

References 37 publications

(98 reference statements)

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“…CD-ECMs from stromal, endothelial and epithelial cells could improve the function of specialized cell types, such as podocytes ( Satyam et al, 2020 ), chondrocytes ( Wei et al, 2015 ; Yang et al, 2018 ; Zhang et al, 2019 ), hepatocytes ( Grant et al, 2017 ; Guo et al, 2017 ), Schwann cells ( Xiao et al, 2016 ), as well as promote natural killer cell differentiation ( Lee et al, 2020 ).…”

Section: Applications Of Cd-ecmmentioning

confidence: 99%

Cell-Derived Extracellular Matrix for Tissue Engineering and Regenerative Medicine

Assunção

Dehghan-Baniani

Yiu

et al. 2020

Front. Bioeng. Biotechnol.

102

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Cell-derived extracellular matrices (CD-ECMs) captured increasing attention since the first studies in the 1980s. The biological resemblance of CD-ECMs to their in vivo counterparts and natural complexity provide them with a prevailing bioactivity. CD-ECMs offer the opportunity to produce microenvironments with costumizable biological and biophysical properties in a controlled setting. As a result, CD-ECMs can improve cellular functions such as stemness or be employed as a platform to study cellular niches in health and disease. Either on their own or integrated with other materials, CD-ECMs can also be utilized as biomaterials to engineer tissues de novo or facilitate endogenous healing and regeneration. This review provides a brief overview over the methodologies used to facilitate CD-ECM deposition and manufacturing. It explores the versatile uses of CD-ECM in fundamental research and therapeutic approaches, while highlighting innovative strategies. Furthermore, current challenges are identified and it is accentuated that advancements in methodologies, as well as innovative interdisciplinary approaches are needed to take CD-ECM-based research to the next level.

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Section: Applications Of Cd-ecmmentioning

confidence: 99%

Cell-Derived Extracellular Matrix for Tissue Engineering and Regenerative Medicine

Assunção

Dehghan-Baniani

Yiu

et al. 2020

Front. Bioeng. Biotechnol.

102

View full text Add to dashboard Cite

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“…Thus, MSC close to the tumor have some features of repairing tissue MSC [ 189 , 190 , 191 ]. Additionally, NK cells come across with several healthy cells before reaching tumor cells [ 192 , 193 , 194 ]. It is conceivable that NK cells may interact with a tumor-budding spheroid during the progressive growth of the neoplastic mass [ 195 , 196 , 197 , 198 ].…”

Section: Tumor Cell Organoids: a More Reliable 3d Model To Study Tumor Cell Biology And Tumor-nk Cells Interactions?mentioning

confidence: 99%

Three-Dimensional Culture Models to Study Innate Anti-Tumor Immune Response: Advantages and Disadvantages

Poggi

Villa

Fernadez

et al. 2021

Cancers

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Several approaches have shown that the immune response against tumors strongly affects patients’ clinical outcome. Thus, the study of anti-tumor immunity is critical to understand and potentiate the mechanisms underlying the elimination of tumor cells. Natural killer (NK) cells are members of innate immunity and represent powerful anti-tumor effectors, able to eliminate tumor cells without a previous sensitization. Thus, the study of their involvement in anti-tumor responses is critical for clinical translation. This analysis has been performed in vitro, co-incubating NK with tumor cells and quantifying the cytotoxic activity of NK cells. In vivo confirmation has been applied to overcome the limits of in vitro testing, however, the innate immunity of mice and humans is different, leading to discrepancies. Different activating receptors on NK cells and counter-ligands on tumor cells are involved in the antitumor response, and innate immunity is strictly dependent on the specific microenvironment where it takes place. Thus, three-dimensional (3D) culture systems, where NK and tumor cells can interact in a tissue-like architecture, have been created. For example, tumor cell spheroids and primary organoids derived from several tumor types, have been used so far to analyze innate immune response, replacing animal models. Herein, we briefly introduce NK cells and analyze and discuss in detail the properties of 3D tumor culture systems and their use for the study of tumor cell interactions with NK cells.

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“…S3A). To monitor NK cell migration we cocultured primary NK cells with EL08.1D2 cells, which have previously been shown to support spontaneous NK cell migration (32,33) and produce extracellular matrix (34), and live imaged them for 24 h capturing photos every 2 minutes (Fig. 3B).…”

Section: Fap Inhibition Reduces Primary Human Nk Cell Migrationmentioning

confidence: 99%

“…2D migration studies were done as previously reported 31,32,65 . In brief, EL08.1D2 stromal cells were grown to a confluent monolayer on flat-bottomed 96 well ImageLock plates (Essen Bioscience) pre-coated with 0.1% gelatin (Stemcell Technologies).…”

Section: D Cell Migration Studiesmentioning

confidence: 99%

Fibroblast activation protein regulates natural killer cell migration, extravasation and tumor infiltration

Fitzgerald

Nasir

et al. 2021

Preprint

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Natural killer (NK) cells play critical roles in physiologic and pathologic conditions such as pregnancy, infection, autoimmune disease and cancer. In cancer, numerous strategies have been designed to exploit the cytolytic properties of NK cells, with variable success. A major hurdle to NK-cell focused therapies is NK cell recruitment to and infiltration into tumors. While the chemotaxis pathways regulating NK recruitment to different tissues are well delineated, the mechanisms human NK cells employ to physically migrate are ill-defined. We show for the first time that human NK cells express fibroblast activation protein (FAP), a cell surface protease previously thought to be primarily expressed by activated fibroblasts. FAP degrades the extracellular matrix to facilitate cell migration and tissue remodeling. We used novel in vivo zebrafish and in vitro 3D culture models to demonstrate that FAP regulates NK cell migration, extravasation, and infiltration into tumors, ultimately affecting tumor cell lysis. These findings demonstrate the necessity of proteolytic migration in NK cell function, suggest novel mechanisms of action of FAP targeting drugs, and provide an entirely new way to regulate NK cell activity.

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Generation of cell-derived matrices that support human NK cell migration and differentiation

Cited by 12 publications

References 37 publications

Cell-Derived Extracellular Matrix for Tissue Engineering and Regenerative Medicine

Cell-Derived Extracellular Matrix for Tissue Engineering and Regenerative Medicine

Three-Dimensional Culture Models to Study Innate Anti-Tumor Immune Response: Advantages and Disadvantages

Fibroblast activation protein regulates natural killer cell migration, extravasation and tumor infiltration

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