Generation of calcium action potentials in crustacean muscle fibers following exposure to sulfhydryl reagents

Zuazaga, C.; Castillo, J. del

doi:10.1016/0742-8413(85)90183-5

Cited by 8 publications

(3 citation statements)

References 22 publications

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“…The relevance of -SH groups in the function of excitable membranes is supported by several publications [63][64][65][66][67][68][69][70]. Exposure to -SH reagents has been reported to cause membrane depolarization [71], and the normally unexcitable ventro-abdominal flexor muscles of the crustacean Atya lanipes generated trains of calcium action potentials after treatments with sulfhydryl reagents [69]. Significantly, Baumgold and coworkers [72] have suggested that -SH groups of proteins located on the axoplasmic side of the axonal plasma membrane may play a role in maintaining excitability because modification of these -SH groups blocks axonal conduction.…”

Section: Why Does Membrane's Osmiophilia Increase In Electrically Sti...mentioning

confidence: 88%

“…Electrical stimulation has been reported to affect -SH groups in squid giant axons, whose -SH groups are unmasked by electrical stimulation, and disulfide groups have been found to play a role in conformational changes induced by electrical stimulation [62]. The relevance of -SH groups in the function of excitable membranes is supported by several publications [63][64][65][66][67][68][69][70]. Exposure to -SH reagents has been reported to cause membrane depolarization [71], and the normally unexcitable ventro-abdominal flexor muscles of the crustacean Atya lanipes generated trains of calcium action potentials after treatments with sulfhydryl reagents [69].…”

Section: Why Does Membrane's Osmiophilia Increase In Electrically Sti...mentioning

confidence: 94%

“…Unmasking of -SH groups is believed to cause conformational changes in proteins, which may be relevant to ion transport and membrane electrical properties during excitation. Zuazaga and coworkers [69,70] have suggested that crustacean muscle excitability is due to the conversion of certain CH2-SH side chains to thioethers with carbonyl groups, which may interact with neighboring amino groups and form bonds between different protein domains or protein units. This might affect excitability by inducing conformational changes or by preventing the occurrence of them.…”

Section: Why Does Membrane's Osmiophilia Increase In Electrically Sti...mentioning

confidence: 99%

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Nerve Structure-Function: Unusual Structural Details and Unmasking of Sulfhydryl Groups by Electrical Stimulation or Asphyxia in Axon Membranes and Gap Junctions

Peracchia

2023

IJMS

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This review describes and discusses unusual axonal structural details and evidence for unmasking sulfhydryl groups (-SH) in axoplasmic membranes resulting from electrical stimulation or asphyxia. Crayfish axons contain fenestrated septa (FS) that, in phase contrast, micrographs appear as repeated striations. In the electron microscope, each septum is made of two cross-sectioned membranes containing ~55 nm pores, each occupied by a microtubule. Thin filaments, which we believe are made of kinesin, bridge the microtubule to the edge of the pore. FS are believed to play a role in axoplasmic flow. The axons also display areas in which axon and sheath glial cell plasma membranes are sharply curved and project into the axoplasm. In freeze-fractures, the protoplasmic leaflet (P-face) of the projections appears as elongated indentations containing parallel chains of particles. The sheath glial cell plasma membrane also contains particles, but they are irregularly aggregated. The axons also display areas where axonal and glial plasma membranes fuse, creating intercellular pores. In axons fixed during electrical stimulation, the plasma membrane, the outer membrane of mitochondria, membranes of other cytoplasmic organelles, and gap junctions increase in electron opacity and thickness, resulting from unmasking of sulfhydryl groups (-SH). Similar changes occur in asphyxiated nerve cords.

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Section: Why Does Membrane's Osmiophilia Increase In Electrically Sti...mentioning

confidence: 88%

Section: Why Does Membrane's Osmiophilia Increase In Electrically Sti...mentioning

confidence: 94%

Section: Why Does Membrane's Osmiophilia Increase In Electrically Sti...mentioning

confidence: 99%

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Nerve Structure-Function: Unusual Structural Details and Unmasking of Sulfhydryl Groups by Electrical Stimulation or Asphyxia in Axon Membranes and Gap Junctions

Peracchia

2023

IJMS

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Sulfhydryl alkylating agents induce calcium current in skeletal muscle fibers of a crustacean (Atya lanipes)

et al. 1992

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Voltage-clamp experiments using the three-microelectrode voltage clamp technique were performed on ventroabdominal flexor muscles of the crustacean Atya lanipes. Potassium and chloride currents were found to underlie the normal, passive response of the muscle. Blocking potassium currents with tetraethylammonium and replacing chloride ions with methanesulfonate did not unmask an inward current. By treating the muscle with the sulfhydryl-alkylating agent 4-cyclopentene-1,3-dione an inward current was detected. The current induced by the agent is carried by Ca2+, since it is abolished in Ca(2+)-free solutions. The induced Ca2+ current is detected at about -40 mV and reaches a mean maximum value of -78 microA/cm2 at ca. -10 mV. At this potential the time to peak is close to 15 msec. The induced Ca2+ current inactivated with 1-sec prepulses which did not elicit detectable Ca2+ current; the fitted hx curve had a midpoint of -38 mV and a steepness of 5.0 mV. Measurements of isometric tension were performed in small bundles of fibers, and the effects of the sulfhydryl-alkylating agents 4-cyclopentene-1,3-dione and N-ethylmaleimide were investigated. Tetanic tension was enhanced in a strictly Ca(2+)-dependent manner by 4-cyclopentene-1,3-dione. The amplitude of K+ contractures increased after treatment with N-ethylmaleimide. It is concluded that Ca2+ channels are made functional by the sulfhydryl-specific reagents and that the increase in tension is probably mediated by an increase in Ca2+ influx through the chemically induced Ca2+ channels.

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Silent Calcium Channels in Skeletal Muscle Fibers of the Crustacean Atya lanipes

Monterrubio

Lizardi

Zuazaga

2000

Journal of Membrane Biology

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Generation of calcium action potentials in crustacean muscle fibers following exposure to sulfhydryl reagents

Cited by 8 publications

References 22 publications

Nerve Structure-Function: Unusual Structural Details and Unmasking of Sulfhydryl Groups by Electrical Stimulation or Asphyxia in Axon Membranes and Gap Junctions

Nerve Structure-Function: Unusual Structural Details and Unmasking of Sulfhydryl Groups by Electrical Stimulation or Asphyxia in Axon Membranes and Gap Junctions

Sulfhydryl alkylating agents induce calcium current in skeletal muscle fibers of a crustacean (Atya lanipes)

Silent Calcium Channels in Skeletal Muscle Fibers of the Crustacean Atya lanipes

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