Generation of blastoids from human parthenogenetic stem cells

Zhong, Ke; Luo, Yuxin; Li, Dan; Min, Zheying; Fan, Yong; Yu, Yang

doi:10.1093/lifemedi/lnad006

Cited by 3 publications

(7 citation statements)

References 36 publications

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“…Zhong et al used parthenogenetic hEPSCs (Pg-hEPSCs) that had been converted from parthenogenetic hESCs for the generation of human blastoids [ 26 ]. They also used the two-step induction protocol previously employed by Fan et al [ 28 ].…”

Section: Human Blastoid Formation and In Vitro Implantation Developmentmentioning

confidence: 99%

“…Pg-hEPSC-derived TE-like cells were mixed with Pg-hEPSC in AggreWell plates, resulting in the formation of blastoids that resembled biparental hEPSC-derived blastoids comprising three-lineage cell types. Given that Pg-hESCs exclusively possess the maternal genome, Pg-hEPSCs blastoids can serve as an ideal model for investigating human diseases with parent-of-origin effects and exploring the function of imprinted genes during embryonic development [ 26 ].…”

Section: Human Blastoid Formation and In Vitro Implantation Developmentmentioning

confidence: 99%

“…In summary, human blastoids can be generated through various methods, including the 3D culturing of naïve hPSCs, conversion of primed and naïve hPSCs, and somatic cell reprogramming [ 24 , 25 , 26 , 27 , 28 ]. Human blastoids exhibit many features of natural blastocysts, including morphological and architectural similarities, the ability to undergo in vitro peri-implantation development, and the capability to derive three stem cell types (ESCs, XENCs, and TSCs) [ 27 , 150 , 151 , 152 ].…”

Section: Human Blastoid Formation and In Vitro Implantation Developmentmentioning

confidence: 99%

“…The resulting human blastoids are similar to natural blastocysts and exhibit the developmental potential to form structures resembling amniotic cavities and yolk sacs. hEPSCs: human expanded potential stem cells; hPSCs: human pluripotent stem cells; TSCs: trophoblast stem cells; EPI: epiblast; TE: trophectoderm; PrE: primitive endoderm; OKSM: Oct4, Klf4, Sox2, and c-Myc; iBlastoid: induced blastoid [ 24 , 25 , 26 , 27 , 28 , 150 , 151 , 152 ].…”

Section: Figurementioning

confidence: 99%

“…The utilization of human stem cells to generate human embryonic models offers an alternative avenue to study human embryonic development. Human ESCs and induced pluripotent stem cells (iPSCs) have been used to study in vitro early embryonic development by forming human blastoids [ 24 , 25 , 26 , 27 , 28 ]. These blastoids effectively mimic they key aspects of pre-implantation development at E5–7, and their further cultivation has demonstrated the potential for developing post-implantation tissues.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

In Vitro Embryogenesis and Gastrulation Using Stem Cells in Mice and Humans

Oh,

Na,

Kang

et al. 2023

IJMS

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During early mammalian embryonic development, fertilized one-cell embryos develop into pre-implantation blastocysts and subsequently establish three germ layers through gastrulation during post-implantation development. In recent years, stem cells have emerged as a powerful tool to study embryogenesis and gastrulation without the need for eggs, allowing for the generation of embryo-like structures known as synthetic embryos or embryoids. These in vitro models closely resemble early embryos in terms of morphology and gene expression and provide a faithful recapitulation of early pre- and post-implantation embryonic development. Synthetic embryos can be generated through a combinatorial culture of three blastocyst-derived stem cell types, such as embryonic stem cells, trophoblast stem cells, and extraembryonic endoderm cells, or totipotent-like stem cells alone. This review provides an overview of the progress and various approaches in studying in vitro embryogenesis and gastrulation in mice and humans using stem cells. Furthermore, recent findings and breakthroughs in synthetic embryos and gastruloids are outlined. Despite ethical considerations, synthetic embryo models hold promise for understanding mammalian (including humans) embryonic development and have potential implications for regenerative medicine and developmental research.

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Section: Human Blastoid Formation and In Vitro Implantation Developmentmentioning

confidence: 99%

Section: Human Blastoid Formation and In Vitro Implantation Developmentmentioning

confidence: 99%

Section: Human Blastoid Formation and In Vitro Implantation Developmentmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In Vitro Embryogenesis and Gastrulation Using Stem Cells in Mice and Humans

Oh,

Na,

Kang

et al. 2023

IJMS

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show abstract

Blastoids derived from pluripotent cells: Current research status and future prospect

Luo,

Qi,

2023

Chin. Sci. Bull.

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<em>In vitro</em> Embryogenesis and Gastrulation using Stem Cells

Oh¹,

Na²,

Kang³

et al. 2023

Preprint

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During early embryonic development, fertilized one-cell embryos develop into preimplantation blastocysts and subsequently establish three germ layers through gastrulation during post-implantation development. In recent years, stem cells have emerged as a powerful tool to study embryogenesis and gastrulation without the need for eggs, allowing the generation of embryo-like structures known as synthetic embryos or embryoids. These in vitro models closely resemble early embryos in terms of morphology and gene expression and provide a faithful recapitulation of early pre- and post-implantation embryonic development. Synthetic embryos can be generated through a combinatorial culture of three blastocyst-derived stem cell types, such as embryonic stem cells, trophoblast stem cells, and extraembryonic endoderm cells, or totipotent-like stem cells alone. This review provides an overview of the progress and various approaches in studying in vitro embryogenesis and gastrulation using stem cells. Furthermore, recent findings and breakthroughs in synthetic embryos and gastruloids are outlined. Despite ethical considerations, synthetic embryo models hold promise for understanding mammalian (including human) embryonic development and have potential implications for regenerative medicine and developmental research.

show abstract

Generation of blastoids from human parthenogenetic stem cells

Cited by 3 publications

References 36 publications

In Vitro Embryogenesis and Gastrulation Using Stem Cells in Mice and Humans

In Vitro Embryogenesis and Gastrulation Using Stem Cells in Mice and Humans

Blastoids derived from pluripotent cells: Current research status and future prospect

<em>In vitro</em> Embryogenesis and Gastrulation using Stem Cells

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