Generation of anti-trenbolone monoclonal antibody and establishment of an indirect competitive enzyme-linked immunosorbent assay for detection of trenbolone in animal tissues, feed and urine

Development and characterization of a chitosan-supported immunoaffinity chromatography column for the selective extraction of methandrostenolone from food and feed samples

“…In the field of food safety, IAC has been widely used in sample pretreatment for residue analysis of mycotoxin, veterinary drugs, pesticides, phycotoxins, process and environmental contaminants and vitamins [5][6][7][8]. Sepharose is commonly used as support matrix for chromatography because of its porosity, chemical and mechanical stability.…”

Determination of folic acid in milk, milk powder and energy drink by an indirect immunoassay

“…As illustrated in Fig. 1, the free carboxylic acid group on FA was linked to amino groups on BSA by a carbodiimide‐modified active ester method,22–24 while the coating antigen FA–OVA was prepared via a mixed acid anhydride reaction. These reactions resulted in an amide bond between the FA molecule and the carrier protein.…”

Development and characterisation of an immunoaffinity column for the selective extraction of methandrostenolone from food and feed samples

“…In contrast, immunoaffinity chromatography is a separation method that takes the advantage of the specific and reversible interaction between antibody and antigen, and has been considered as one of the most powerful techniques for isolation and purification of individual or classes of compounds from liquid matrices (Senyuva & Gilbert, 2010). Owing to the advantages of high specificity, simple operation and therefore low cost per sample, immunoaffinity chromatography has been widely used in sample pretreatment for residue analysis of mycotoxins (Klinglmayr, Nöbauer, Razzazi-Fazeli, & Cichna-Markl, 2010;Tavčar-Kalcher, Vrtač, Pestevšek, & Vengušt, 2007;), veterinary drugs (Wang & Shen, 2007;Zhang et al, 2011), pesticides (Chen, Wang, Wang, & Tang, 2009;Wang et al, 2011) and heavy metals (Abe et al, 2011). Our previous work has described an immunoaffinity extraction of MA from food and feed samples using a polyclonal antibody (pAb) against MA (Wang, Xu, Zhang, Wang, & Dong, 2011a).…”