Generation of a soybean BAC library, and identification of DNA sequences tightly linked to the Rps1-k disease resistance gene

Two large-insert soybean genomic libraries constructed in a binary vector: applications in chromosome walking and genome wide physical mapping

“…BAC libraries are simpler to construct, manipulate and maintain than YAC libraries (Frijters et al 1997;Yang et al 1997) and do not suffer problems associated with homologous recombination and differential colony growth. BAC libraries have been constructed for several plant species (Woo et al 1994;Wang et al 1995;Frijters et al 1996;Zhang et al 1997;Bent et al 1998;Vinatzer et al 1998) including soybean (Marek and Shoemaker 1997, Danesh et al 1998, Salimath and Bhattacharyya 1999. During positional cloning, large-insert libraries are often used to integrate fine genetic maps with physical maps.…”

Construction and characterization of a partial binary bacterial artificial chromosome (BIBAC) of Agave tequilana var. azul (2X) and its application for gene identification

“…Genomic libraries of soybean have an average insert size of 105 Kb (Salimath and Bhattacharyya, 1999), 120 Kb (Danesh et al, 1998) and 136 Kb (Tomkins et al, 1999b). The average insert size estimated in the genomic library of rice was found to be of 125 Kb (Wang et al, 1995), 130 Kb in sugar cane (Tomkins et al, 1999a) and of 155 Kb in potato (Song et al, 2000).…”

“…Important features of this type of DNA delivery is the Escherichia coli factor F' allowing for strict control of copy number and unidirectional DNA replication, the latter property promoting plasmid stability compared to YAC having poor stability (Willets and Skurry, 1987 BAC technology has had various applications, such as the transformation of clusters of genes of interest in plant cells (Hamilton et al, 1996(Hamilton et al, , 1999Liu et al, 1999Liu et al, , 2002He et al, 2003), the construction of contigs (Marra et al, 1997;Boysen et al, 1997), development of molecular markers (Wang et al, 1996;Nakamura et al, 1997;Yang et al, 1997;Cai et al, 1998;Danesh et al, 1998) and even the isolation and molecular characterization of repetitive DNA sequences. Given the wide range of applications of the BAC vectors, these have been developed in a variety of important crops such as tomato (Choi et al, 1995), maize (Mozo et al, 1998;Folkertsma et al, 1999), soybean (Salimath and Bhattacharyya, 1999;Danesh et al, 1998;Tomkins et al, 1999b), rice (Wang et al, 1995) sugar cane (Tomkins et al, 1999a) and potato (Song et al, 2000).…”

Construction and Characterization of a Lipotes vexillifer Genomic DNA BAC Library

“…To accomplish these goals, genomic DNA libraries have been constructed using large-insert cloning systems such as YACs (Burke et al, 1987), BAC (Shizuya et al, 1992(Shizuya et al, , 2001 and PACs (Ioannou et al, 1994). In particular, BAC libraries have contributed enormously to the success of genome sequencing and mapping (Adams et al, 2000;Lin et al, 1999;Mayer et al, 1999;Dunham et al, 1999;Hattori et al, 2000), gene cloning Salimath and Bhattacharyya, 1999;Vinatzer et al, 1998;Hohmann et al, 2003;Nam et al, 1999;Li et al, 1999), and gene prediction efforts (Nielsen et al, 1997;Antoch et al, 1997).…”