Generation of a Rat Model of Acute Liver Failure by Combining 70% Partial Hepatectomy and Acetaminophen

Sahay, Preeti; Jain, Kshama; Sinha, Prakriti; Das, Balaram; Mishra, Alaknanda; Kesarwani, Ashwani; Sahu, Parul; Mohan, K. Varsha; Kumar, Mahesh; Nagarajan, Priyadharsini; Upadhyay, Pramod

doi:10.3791/60146

Cited by 6 publications

(4 citation statements)

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“…Despite this, several studies report the use of rats as an APAP-toxicity model with doses ranging from 400 mg/kg IP to 5 g/kg PO ( Panatto et al, 2011 ; Mahmoud & Mahmoud, 2016 ). The lower toxicity of APAP in rats may be offset by combining it with other forms of acute (partial hepatectomy) or chronic (non-alcoholic steatohepatitis) hepatic injury ( Kučera et al, 2012 ; Sahay et al, 2019 ).…”

Section: Survey Methodologymentioning

confidence: 99%

Preclinical models of acute liver failure: a comprehensive review

Hefler

Marfil‐Garza

Pawlick

et al. 2021

PeerJ

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Acute liver failure is marked by the rapid deterioration of liver function in a previously well patient over period of days to weeks. Though relatively rare, it is associated with high morbidity and mortality. This makes it a challenging disease to study clinically, necessitating reliance on preclinical models as means to explore pathophysiology and novel therapies. Preclinical models of acute liver failure are artificial by nature, and generally fall into one of three categories: surgical, pharmacologic or immunogenic. This article reviews preclinical models of acute liver failure and considers their relevance in modeling clinical disease.

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Section: Survey Methodologymentioning

confidence: 99%

Preclinical models of acute liver failure: a comprehensive review

Hefler

Marfil‐Garza

Pawlick

et al. 2021

PeerJ

View full text Add to dashboard Cite

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“…Removal of 70% of the liver mass, named 70% partial hepatectomy, was performed as described by Higgins and Anderson, 1931. 14,15 Vitamin C (250 mg/kg BW) administration were given orally via oral gavage for 6 consecutive days after the animals successfully recovered from 70% PHx. Daily dose was administered at approximately the same time each day.…”

Section: Methodsmentioning

confidence: 99%

Effect of vitamin C on liver regeneration after partial hepatectomy and acetaminophen-induced liver injury in Wistar rats

2021

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Background: The aim of this study is to evaluate the potential effect of vitamin C as an antioxidant on liver regeneration after partial hepatectomy and acetaminophen-induced liver injury in Wistar rats.Methods: A total of 24 male Wistar rats were divided into four groups, each group consisted of 6 rats: group A (control, partial hepatectomy/PHx alone), group B (PHx and vitamin C 250 mg/kg BW), group C (acetaminophen 500 mg/kg BW and PHx), and group D (acetaminophen 500 mg/kg BW with PHx and vitamin C 250 mg/kg BW). Subtoxic dose of acetaminophen was given 24 hours before partial hepatectomy. Vitamin C was given orally via oral gavage for 6 consecutive days after partial hepatectomy. POD 7, all animals were terminated and performed laparotomy to obtain liver tissue for measurement of liver weight and regeneration rate, blood samples for malondialdehyde (MDA) as a lipid peroxidation measurement and histopathological investigation.Results: The means of regeneration rate in vitamin C groups were significantly higher compared to non-vitamin C group (p<0.05). Similar result, the means of MDA values in vitamin C groups were significantly lower compared to non-vitamin C group (p<0.05). This result suggests a protective effect of vitamin C against lipid peroxidation. Histopathological changes in liver cells were statistically difference between vitamin C groups and non-vitamin C groups (p<0.05).Conclusions: Our results indicate that vitamin C administration promotes liver regeneration and inhibits lipid peroxidation after partial hepatectomy and acetaminophen-induced liver injury in Wistar rats.

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“…Until now, accumulated advances in research have led to significant achievements in understanding the characteristics and progress of hepatectomy, as a result of tumors, cirrhosis, and liver failure. Sahay et al ( 2 ) established an acute liver failure model in rats by performing 70% partial hepatectomy (PHx) and consequently evaluated the therapeutic potential of cells in bridging the gap between acute liver failure and liver transplantation. The PHx rat model has been widely used since then to study the involvement of regeneration in the recovery mechanism from acute liver injury ( 3 ).…”

Section: Introductionmentioning

confidence: 99%