Generation of a ChAT mouse line without the early onset hearing loss typical of the C57BL/6J strain

Beebe, Nichole L.; Sowick, Colleen S.; Kristaponyte, Inga; Galazyuk, Alexander V.; Vetter, Douglas E.; Cox, Brandon C.; Schofield, Brett R.

doi:10.1016/j.heares.2020.107896

Cited by 3 publications

(1 citation statement)

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“…The new strains thus provide new tools for studying circuit mechanisms of auditory processing and multisensory integration across the lifespan without potential confounds due to progressive AHL. Similar approaches were used by Beebe et al (2020) and Lyngholm and Sakata (2019) to generate ChAT-Cre and Cre-dependent channelrhodopsin2 strains without AHL.…”

Section: Discussionmentioning

confidence: 99%

Sst- and Vip-Cre mouse lines without age-related hearing loss

Foss,

Olsen,

Bigelow

et al. 2024

Preprint

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GABAergic interneurons, including somatostatin (SST) and vasoactive intestinal peptide (VIP) positive cells, play a crucial role in cortical circuit processing. Cre recombinase-mediated manipulation of these interneurons is facilitated by commercially available knock-in mouse strains such as Sst-IRES-Cre (Sst-Cre) and Vip-IRES-Cre (Vip-Cre). However, these strains are troublesome for hearing research because they are only available on the C57BL/6 genetic background, which suffer from early onset age-related hearing loss (AHL) due to a mutation of the Cdh23 gene. To overcome this limitation, we backcrossed Sst-Cre and Vip-Cre mice to CBA mice to create normal-hearing offspring with the desired Cre transgenes. We confirmed that in these “CBA Cre” lines, Cre drives appropriate expression of Cre-dependent genes, by crossing CBA Cre mice to Ai14 reporter mice. To assess the hearing capabilities of the CBA Cre mice, we measured auditory brainstem responses (ABRs) using clicks and tones. CBA Cre mice showed significantly lower ABR thresholds compared to C57 control mice at 3, 6, 9, and 12 months. In conclusion, our study successfully generated Sst-Cre and Vip-Cre mouse lines on the CBA background that will be valuable tools for investigating the roles of SST and VIP positive interneurons without the confounding effects of age-related hearing loss.

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Section: Discussionmentioning

confidence: 99%