Generation, Endothelialization, and Microsurgical Suture Anastomosis of Strong 1-mm-Diameter Collagen Tubes

Li, Xuanyue; Xu, Jing; Nicolescu, Calin; Marinelli, Jordann T; Tien, Joe

doi:10.1089/ten.tea.2016.0339

Cited by 36 publications

(38 citation statements)

References 38 publications

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“…Mechanical failure from material fatigue and subsequent death led to clotting in the graft lumen. In all cases, the grafts were able to withstand the high-pressure blood flow after the surgical clamps were released and even withstood aortic flow for several days without bursting or leaking, a longer period than previously tested on natural polymer grafts (56). Initial successful in vivo implantation indicates surgical utility of the fibrin microfiber tube.…”

Section: Development Of Natural Polymer Conduits With Controlled Surfacementioning

confidence: 82%

Regenerative and durable small-diameter graft as an arterial conduit

Elliott

Ginn

Fukunishi

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Despite significant research efforts, clinical practice for arterial bypass surgery has been stagnant, and engineered grafts continue to face postimplantation challenges. Here, we describe the development and application of a durable small-diameter vascular graft with tailored regenerative capacity. We fabricated small-diameter vascular grafts by electrospinning fibrin tubes and poly(e-caprolactone) fibrous sheaths, which improved suture retention strength and enabled long-term survival. Using surface topography in a hollow fibrin microfiber tube, we enable immediate, controlled perfusion and formation of a confluent endothelium within 3-4 days in vitro with human endothelial colony-forming cells, but a stable endothelium is noticeable at 4 weeks in vivo. Implantation of acellular or endothelialized fibrin grafts with an external ultrathin poly(e-caprolactone) sheath as an interposition graft in the abdominal aorta of a severe combined immunodeficient Beige mouse model supports normal blood flow and vessel patency for 24 weeks. Mechanical properties of the implanted grafts closely approximate the native abdominal aorta properties after just 1 week in vivo. Fibrin mediated cellular remodeling, stable tunica intima and media formation, and abundant matrix deposition with organized collagen layers and wavy elastin lamellae. Endothelialized grafts evidenced controlled healthy remodeling with delayed and reduced macrophage infiltration alongside neo vasa vasorum-like structure formation, reduced calcification, and accelerated tunica media formation. Our studies establish a small-diameter graft that is fabricated in less than 1 week, mediates neotissue formation and incorporation into the native tissue, and matches the native vessel size and mechanical properties, overcoming main challenges in arterial bypass surgery.small-diameter vascular graft | infrarenal abdominal aorta mouse model | electrospinning | hydrogel | endothelial colony-forming cells

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Section: Development Of Natural Polymer Conduits With Controlled Surfacementioning

confidence: 82%

Regenerative and durable small-diameter graft as an arterial conduit

Elliott

Ginn

Fukunishi

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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“…Collagen-based vascular grafts were recently tested in vivo and assessed for the feasibility of the system to hold microsurgical sutures. Dehydration of crosslinked collagen allowed to create a small-diameter TEVG (diameter ≤ 1 mm) with a burst pressure of approximately 1313 mmHg, compliance of 1.7%/100 mmHg (comparable with mammalian vein), but the strength of the anastomosis at the interface between the rat femoral artery and TEVG was still lower than the one between two portions of explanted rat femoral artery (Li et al, 2017 ). Natural polymers are acknowledged to be valid alternatives in the production of small diameter TEVGs, due to their higher biocompatibility and capability to remodel in vivo .…”

Section: Tissue Engineeringmentioning

confidence: 99%

Current Strategies for the Manufacture of Small Size Tissue Engineering Vascular Grafts

Carrabba

Madeddu

2018

Front. Bioeng. Biotechnol.

171

144

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Occlusive arterial disease, including coronary heart disease (CHD) and peripheral arterial disease (PAD), is the main cause of death, with an annual mortality incidence predicted to rise to 23.3 million worldwide by 2030. Current revascularization techniques consist of angioplasty, placement of a stent, or surgical bypass grafting. Autologous vessels, such as the saphenous vein and internal thoracic artery, represent the gold standard grafts for small-diameter vessels. However, they require invasive harvesting and are often unavailable. Synthetic vascular grafts represent an alternative to autologous vessels. These grafts have shown satisfactory long-term results for replacement of large- and medium-diameter arteries, such as the carotid or common femoral artery, but have poor patency rates when applied to small-diameter vessels, such as coronary arteries and arteries below the knee. Considering the limitations of current vascular bypass conduits, a tissue-engineered vascular graft (TEVG) with the ability to grow, remodel, and repair in vivo presents a potential solution for the future of vascular surgery. Here, we review the different methods that research groups have been investigating to create TEVGs in the last decades. We focus on the techniques employed in the manufacturing process of the grafts and categorize the approaches as scaffold-based (synthetic, natural, or hybrid) or self-assembled (cell-sheet, microtissue aggregation and bioprinting). Moreover, we highlight the attempts made so far to translate this new strategy from the bench to the bedside.

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“…These hydrogel-based scaffolds have been successfully used for the development of small-diameter vascular grafts. [34][35][36] In addition to ECM, decellularized natural matrices derived from animal tissues have also shown their potential in constructing TEVGs (Fig. 2).…”

Section: Synthetic and Natural Substrates Synthetic Substrates Can Bmentioning

confidence: 99%

Vascular Tissue Engineering: Advanced Techniques and Gene Editing in Stem Cells for Graft Generation

Chen

Ugwu

Li³

et al. 2021

Tissue Engineering Part B: Reviews

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The common occurrence of cardiovascular diseases and the lack of proper autologous tissues prompt and promote the pressing development of tissue-engineered vascular grafts. Current progress on scaffold production, genetically modified cells and use of nanotechnology-based monitoring has considerably improved the long-term patency of engineered tissue grafts. However, challenges abound in the autologous materials and manipulation of genes and cells for tissue engineering. This review overviews current development in tissue-engineered vascular grafts and discusses recent improvements in scaffolding techniques as well as the efficiency of gene-editing tools and their ability to fill the existing gaps in stem-cell and regenerative therapies. Current advances in 3D-printing approaches for fabrication of engineered tissues are also reviewed together with specific biomaterials for vascular tissues. In addition, the natural and synthetic polymers that hold increasing significance for vascular tissue engineering are highlighted. Both animal models and nanotechnology-based monitoring are proposed for pre-clinical evaluation of engineered grafts in view of their historical significance in tissue engineering. The ultimate success of tissue regeneration, which is yet to be fully realized, depends on the optimal performance of culture systems, biomaterial constructs and stem cells in a suitable artificial physiological environment.

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Generation, Endothelialization, and Microsurgical Suture Anastomosis of Strong 1-mm-Diameter Collagen Tubes

Cited by 36 publications

References 38 publications

Regenerative and durable small-diameter graft as an arterial conduit

Regenerative and durable small-diameter graft as an arterial conduit

Current Strategies for the Manufacture of Small Size Tissue Engineering Vascular Grafts

Vascular Tissue Engineering: Advanced Techniques and Gene Editing in Stem Cells for Graft Generation

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