1988
DOI: 10.1007/978-3-642-73217-1_12
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Generation, Biology, and Assay of Efferent Lymphokines

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(2 citation statements)
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“…Serum levels of TNF-(Y were determined by a standard cytotoxicity bio-assay using actinomycin-D-treated L929 fibroblasts as targets (Gemsa, 1988). These cells were kindly provided by Dr. Y. Keisari (Tel-Aviv University).…”
Section: Tnf Levelsmentioning
confidence: 99%
See 1 more Smart Citation
“…Serum levels of TNF-(Y were determined by a standard cytotoxicity bio-assay using actinomycin-D-treated L929 fibroblasts as targets (Gemsa, 1988). These cells were kindly provided by Dr. Y. Keisari (Tel-Aviv University).…”
Section: Tnf Levelsmentioning
confidence: 99%
“…Briefly, 50 PI of different dilutions of each serum sample were incubated for 24 hr at 37°C with 2.5 x lo4 L929 cells in flat-bottomed wells of 96-well plates. Cytotoxicity was measured by an MTT colorimetric assay (Gemsa, 1988) using a Dynatec automatic spectrophotometer. Units of TNF are presented as the reciprocal of the highest supernatant dilution which results in 40% lysis of a L929 cell monolayer, multiplied by the dilution factor and compared to a standard curve of recombinant TNF-a to estimate TNF activity.…”
Section: Tnf Levelsmentioning
confidence: 99%