Generation, Biology, and Assay of Efferent Lymphokines

Gemsa, Diethard

doi:10.1007/978-3-642-73217-1_12

Cited by 1 publication

(2 citation statements)

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“…Serum levels of TNF-(Y were determined by a standard cytotoxicity bio-assay using actinomycin-D-treated L929 fibroblasts as targets (Gemsa, 1988). These cells were kindly provided by Dr. Y. Keisari (Tel-Aviv University).…”

Section: Tnf Levelsmentioning

confidence: 99%

“…Briefly, 50 PI of different dilutions of each serum sample were incubated for 24 hr at 37°C with 2.5 x lo4 L929 cells in flat-bottomed wells of 96-well plates. Cytotoxicity was measured by an MTT colorimetric assay (Gemsa, 1988) using a Dynatec automatic spectrophotometer. Units of TNF are presented as the reciprocal of the highest supernatant dilution which results in 40% lysis of a L929 cell monolayer, multiplied by the dilution factor and compared to a standard curve of recombinant TNF-a to estimate TNF activity.…”

Section: Tnf Levelsmentioning

confidence: 99%

See 1 more Smart Citation

Myeloproliferation in long‐term plasmacytoma‐regressor mice

Sagi‐Assif

Douer

Shaked

et al. 1994

Intl Journal of Cancer

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MOPC-315 plasmacytoma-bearing BALB/c mice were treated with high doses of melphalan, causing a permanent and complete regression of the tumor. In the present study we analyzed plasmacytoma-regressor mice (PRM) 3-6 months after plasmacytoma regression. A second group of otherwise untreated normal mice was treated with melphalan (M--control group). A third group of mice remained untreated and served as an age- and sex-matched control group. PRM were cachectic and had an increased mortality rate compared to the M and the C control groups. Histopathological examination indicated that the spleen of PRM showed pronounced abnormalities, primarily in the red pulp. These abnormalities consisted of extramedullary hematopoiesis and myeloid-granulocytic hyperplasia. Spleens of M mice showed similar abnormalities but to a much lesser extent. Flow cytometric analysis of cellular surface markers of PRM splenocytes indicated a high number of large MAC-I- and GR-I-positive cells compared to splenocytes of M or C controls. These large cells also expressed Fc tau receptors (Fc tau RII), stained positively with non-specific esterase and adhered to plastic dishes; a certain percentage expressed MAC-2 and MAC-3 antigens. A quantitative suppression of CD4+ T cells and of B cells was also shown. Circulating levels of TNF were higher in PRM than in M or C mice. The capacity of splenocytes from PRM to secrete factors that stimulated CFU-GM colony formation in soft agar by bone-marrow cells from normal mice was significantly up-regulated compared to that of splenocytes from M or C mice.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Tnf Levelsmentioning

confidence: 99%

Section: Tnf Levelsmentioning

confidence: 99%