Generation and selection of antibodies for a novel immunochromatographic lateral flow test to rapidly identify OXA-23-like-mediated carbapenem resistance in Acinetobacter baumannii

Mertins, Sonja; Higgins, Paul G.; Rodríguez, María González; Borlon, Céline; Gilleman, Quentin; Mertens, Pascal; Seifert, Harald; Krönke, Martin; Klimka, Alexander

doi:10.1099/jmm.0.001015

Cited by 8 publications

(32 citation statements)

References 25 publications

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“…Either the antibodies or the proteins can be labelled with fluorescent tags for visualization. The interaction between the antibodies and resistance enzymes requires optimization, but several lateral flow assays (LFAs) have been developed for the detection of β lactamases 72 . An LFA specific to New Delhi metallo βlactamase (NDM) 1 was 100% sensitive and specific in a collection of 350 isolates from Myanmar 73 .…”

Section: Detection Of Protein Markers Of Resistancementioning

confidence: 99%

Innovative and rapid antimicrobial susceptibility testing systems

Belkum

Burnham

Rossen³

et al. 2020

Nat Rev Microbiol

211

154

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| Antimicrobial resistance (AMR) is a major threat to human health worldwide, and the rapid detection and quantification of resistance, combined with antimicrobial stewardship, are key interventions to combat the spread and emergence of AMR . Antimicrobial susceptibility testing (AST) systems are the collective set of diagnostic processes that facilitate the phenotypic and genotypic assessment of AMR and antibiotic susceptibility. Over the past 30 years, only a few high-throughput AST methods have been developed and widely implemented. By contrast, several studies have established proof of principle for various innovative AST methods, including both molecular-based and genome-based methods, which await clinical trials and regulatory review. In this Review , we discuss the current state of AST systems in the broadest technical, translational and implementation-related scope.

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Section: Detection Of Protein Markers Of Resistancementioning

confidence: 99%

Innovative and rapid antimicrobial susceptibility testing systems

Belkum

Burnham

Rossen³

et al. 2020

Nat Rev Microbiol

211

154

View full text Add to dashboard Cite

show abstract

“…Then, the bacteria were diluted to a concentration of around 5 × 10 5 CFU/mL (standard concentration used for AST) in the M9 minimal medium. The M9 minimal medium was supplemented with either 12 C-glucose or 13 C-glucose at a concentration of 0.2% w/v. The 13 C-glucose (D-Glucose U-13 C 6 , 99%, Cambridge Isotope Laboratories) was universally labeled where all carbon atoms were replaced with 13 C atoms.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…The M9 minimal medium was supplemented with either 12 C-glucose or 13 C-glucose at a concentration of 0.2% w/v. The 13 C-glucose (D-Glucose U-13 C 6 , 99%, Cambridge Isotope Laboratories) was universally labeled where all carbon atoms were replaced with 13 C atoms. The antibiotics (gentamicin, ampicillin, trimethoprim, and erythromycin) were dissolved with either DMSO or PBS as the stock solutions and added at the clinical breakpoint concentration.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…Great efforts have been made to reduce the diagnostic time down to within a working shift (<8 h) for rapid AST. − Molecular-based AST methods detect known genes or protein markers of resistance. − However, non-characterized resistance mechanisms or new mutations would remain elusive. Additionally, the absence of resistance markers cannot reliably predict phenotypic susceptibility.…”

Section: Introductionmentioning

confidence: 99%

“…57 We systematically design and implement a highly sensitive wide-field MIP microscope, achieving high-throughput metabolic imaging of hundreds of individual bacterial cells within seconds. Then, we quantify protein synthesis from isotopically labeled glucose ( 13 Cglucose) in terms of the 13 C-protein replacement ratio using dual-wavenumber MIP imaging at the original (1656 cm −1 ) and shifted amide I (1612 cm −1 ) peaks. Finally, we demonstrate that the 13 C-protein replacement ratio is a reliable metabolic marker for the determination of susceptibility of E. coli against various antibiotics with as short as 1 h treatment.…”

Section: ■ Introductionmentioning

confidence: 99%

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High-Throughput Antimicrobial Susceptibility Testing of Escherichia coli by Wide-Field Mid-Infrared Photothermal Imaging of Protein Synthesis

et al. 2023

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Antimicrobial resistance poses great threats to global health and economics. Current gold-standard antimicrobial susceptibility testing (AST) requires extensive culture time (36−72 h) to determine susceptibility. There is an urgent need for rapid AST methods to slow down antimicrobial resistance. Here, we present a rapid AST method based on wide-field mid-infrared photothermal imaging of protein synthesis from 13 C-glucose in Escherichia coli. Our wide-field approach achieved metabolic imaging for hundreds of bacteria at the single-cell resolution within seconds. The perturbed microbial protein synthesis can be probed within 1 h after antibiotic treatment in E. coli cells. The susceptibility of antibiotics with various mechanisms of action has been probed through monitoring protein synthesis, which promises great potential of the proposed platform toward clinical translation.

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Antimicrobial Resistance in Rivers: A Review of the Genes Detected and New Challenges

Grenni

2022

Enviro Toxic and Chemistry

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River ecosystems are very important parts of the water cycle and an excellent habitat, food, and drinking water source for many organisms, including humans. Antibiotics are emerging contaminants which can enter rivers from various sources. Several antibiotics and their related antibiotic resistance genes (ARGs) have been detected in these ecosystems by various research programs and could constitute a substantial problem. The presence of antibiotics and other resistance cofactors can boost the development of ARGs in the chromosomes or mobile genetic elements of natural bacteria in rivers. The ARGs in environmental bacteria can also be transferred to clinically important pathogens. However, antibiotics and their resistance genes are both not currently monitored by national or international authorities responsible for controlling the quality of water bodies. For example, they are not included in the contaminant list in the European Water Framework Directive or in the US list of Water-Quality Benchmarks for Contaminants. Although ARGs are naturally present in the environment, very few studies have focused on non-impacted rivers to assess the background ARG levels in rivers, which could provide some useful indications for future environmental regulation and legislation. The present study reviews the antibiotics and associated ARGs most commonly measured and detected in rivers, including the primary analysis tools used for their assessment. In addition, other factors that could enhance antibiotic resistance, such as the effects of chemical mixtures, the effects of climate change, and the potential effects of the coronavirus disease 2019 pandemic, are discussed.

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Generation and selection of antibodies for a novel immunochromatographic lateral flow test to rapidly identify OXA-23-like-mediated carbapenem resistance in Acinetobacter baumannii

Cited by 8 publications

References 25 publications

Innovative and rapid antimicrobial susceptibility testing systems

Innovative and rapid antimicrobial susceptibility testing systems

High-Throughput Antimicrobial Susceptibility Testing of Escherichia coli by Wide-Field Mid-Infrared Photothermal Imaging of Protein Synthesis

Antimicrobial Resistance in Rivers: A Review of the Genes Detected and New Challenges

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