Generation and Properties of Antibacterial Coatings Based on Electrostatic Attachment of Silver Nanoparticles to Protein-Coated Polypropylene Fibers

Abstract

We present a simple method for attaching silver nanoparticles to polypropylene (PP) fibers in a two-step process to impart antibacterial properties. Specifically, PP fibers are pretreated by the adsorption from an aqueous solution of heat-denatured lysozyme (LYS) followed by LYS cross-linking using glutaraldehyde and sodium borohydride. At neutral pH, the surface of the adsorbed LYS layer is enriched with numerous positive charges. Silver nanoparticles (AgNPs) capped with trisodium citrate are subsequently deposited onto the protein-coated PP. Nanoparticle binding is mediated by electrostatic interactions between the positively charged LYS layer and the negatively charged AgNPs. The density of AgNPs deposited on PP depends on the amount of protein adsorbed on the surface. UV-vis spectroscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, and scanning electron microscopy are employed to follow all preparation steps and to characterize the resulting functional surfaces. The antibacterial activity of the modified surfaces is tested against gram negative bacteria Escherichia coli (E. coli). Overall, our results show that PP surfaces coated with AgNPs exhibit excellent antibacterial activity with 100% removal efficiency.

The preparation of alginate–AgNPs composite fiber with green approach and its antibacterial activity

“…Nanomaterials can play an important role in antibacterial applications primarily due to their large surface area and size/ shape-dependent physicochemical properties [3][4][5]. Among various antibacterial nanomaterials, silver nanoparticles (AgNPs) are so far the most exploited one as they can insure extraordinary antimicrobial efficacy against a wide range of microorganisms including bacteria, fungi, viruses, etc.…”

Unaffected features of BSA stabilized Ag nanoparticles after storage and reconstitution in biological relevant media

“…Biofunctionalization is very important to achieve biocompatibility, which is one of the primary criteria to use Ag-NP in orthopedic implants. Further, NP adhesion to the surface of biomaterials may be improved by protein mediated interactions [26]. Finally, a critical but often overlooked drawback of Ag-NP suspensions involves their short-and long-term storage due to oxidation and photoassisted reactions [27,28].…”

Effective surface attachment of Ag nanoparticles on fibers using glycidyltrimethylammonium chloride and improvement of antimicrobial properties

“…[31][32][33][34] Even though the detailed mechanism has not been fully elucidated, the particle size, shape, crystallinity, material surrounding the particles, pH of the solution, ionic strengths, types of ions, and ligand are signicant factors inuencing the antimicrobial activity of AgNPs. 35,36 It is generally known that the smaller the size of the AgNPs, the greater is the number of atoms on the surface that can come into contact with bacteria, and the greater amount of silver ions can be released.…”