Generation and cryopreservation of feline oviductal organoids

Thompson, Riley E.; Meyers, Mindy A.; Premanandan, Christopher; FK, Hollinshead

doi:10.1016/j.theriogenology.2022.11.020

Cited by 3 publications

(1 citation statement)

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“…For overcoming this limitation, we have tried to produce islet organoids derived from porcine islets. Some studies have revealed that organoids can be cultured for a long term, proliferated and cryopreserved 16 – 18 . If the islet organoids harbor similar characteristics to porcine islets, they can be used as considerable donor of transplant therapy for DM.…”

Section: Introductionmentioning

confidence: 99%

The porcine islet-derived organoid showed the characteristics as pancreatic duct

Sakata,

Yoshimatsu,

Kawakami

et al. 2024

Sci Rep

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Organoid is a tissue-engineered organ-like structure that resemble as an organ. Porcine islet-derived organoid might be used as an alternative donor of porcine islet xenotransplantation, a promising therapy for severe diabetes. In this study, we elucidated the characteristics of porcine islet organoids derived from porcine islets as a cell source for transplantation. Isolated porcine islets were 3D-cultured using growth factor-reduced matrigel in organoid culture medium consist of advanced DMEM/F12 with Wnt-3A, R-spondin, EGF, Noggin, IGF-1, bFGF, nicotinamide, B27, and some small molecules. Morphological and functional characteristics of islet organoids were evaluated in comparison with 2D-cultured islets in advanced DMEM/F12 medium. Relatively short-term (approximately 14 days)—cultured porcine islet organoids were enlarged and proliferated, but had an attenuated insulin-releasing function. Long-term (over a month)—cultured islet organoids could be passaged and cryopreserved. However, they showed pancreatic duct characteristics, including cystic induction, strong expression of Sox9, loss of PDX1 expression, and no insulin-releasing function. These findings were seen in long-term-cultured porcine islets. In conclusion, our porcine islet organoids showed the characteristics of pancreatic ducts. Further study is necessary for producing porcine islet-derived organoids having characteristics as islets.

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Section: Introductionmentioning

confidence: 99%

The porcine islet-derived organoid showed the characteristics as pancreatic duct

Sakata,

Yoshimatsu,

Kawakami

et al. 2024

Sci Rep

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Bovine oviductal organoids: a multi-omics approach to capture the cellular and extracellular molecular response of the oviduct to heat stress

Menjivar,

Gad,

Thompson

et al. 2023

BMC Genomics

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Background The mammalian oviduct is a complex, fibromuscular organ known for its role in orchestrating a series of timely and dynamic changes to suitably support early embryogenesis. Climate change-induced heat stress (HS) is one of the largest single stressors compromising reproductive function in humans and farm animals via systemic changes in the redox status of the maternal environment, adversely affecting fertilization and early embryonic development. Oviductal organoids represent a unique 3-dimensional, biomimetic model to study the physiology of the oviduct and its subsequent impact on embryo development under various environmental conditions. Results Our study is the first to demonstrate an innovative approach to understanding the cascade of molecular changes sustained by bovine oviductal organoids under HS and the subsequent maternal signals harnessed within their secreted extracellular vesicles (EVs). Transcriptomic analysis of oviductal organoids exposed to HS revealed 2,570 differentially expressed genes (1,222 up‐ and 1,348 downregulated), while EV-coupled miRNome analysis disclosed 18 miRNAs with significant differential expression (12 up- and 6 downregulated) in EVs from thermally stressed organoids compared to EVs released from organoids cultured under thermoneutral conditions. Genes activated in oviductal organoids in response to thermal stress, include: COX1, ACTB, CST6, TPT1, and HSPB1, while miR-1246, miR-148a, miR21-5p, miR-451, and miR-92a represent the top highly abundant EV-coupled miRNAs released in response to HS. Pathway analysis of genes enriched in organoids exposed to thermal stress showed the enrichment of endocrine resistance, cellular senescence, and notch signaling pathways. Similarly, EV-coupled miRNAs released from thermally stressed organoids showed their potential regulation of genes involved in cellular senescence, p53 signaling, and TGF-beta signaling pathways. Conclusions In conclusion, the cellular and extracellular response of bovine oviductal organoids to in vitro HS conditions reveal the prospective impact of environmental HS on the physiology of the oviduct and the probable subsequent impacts on oocyte fertilization and early embryo development. Future studies elucidating the potential impact of HS-associated EVs from oviductal organoids on oocyte fertilization and preimplantation embryo development, would justify the use of an organoid model to optimally understand the oviduct-embryo communication under suboptimal environments.

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La pandemia de COVID-19 como oportunidad para poner de relieve la migración de los trabajadores agrícolas a través de la cobertura mediática

Șerban

Croitoru

2022

Estud. geogr.

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En los últimos años, el interés por las representaciones mediáticas de los emigrantes y los medios de comunicación como espacio de participación ha aumentado dentro del campo de los estudios migratorios. Sin embargo, la mayor parte de la atención de los estudiosos se centra en los inmigrantes y los medios de comunicación en los países de destino, mientras que se presta menos atención a los países de origen y a la representación de los emigrantes. Aprovechando la mayor atención prestada a los emigrantes y a la migración durante la primera fase de la pandemia del COVID-19, investigamos las representaciones mediáticas de los emigrantes rumanos en la agricultura que trabajan en otros países europeos e interpretamos cómo sus voces podían ser escuchadas a través de los relatos mediáticos. Mediante un análisis de contenido, investigamos una muestra de 297 artículos publicados entre el 1 de abril y el 31 de mayo de 2020 en los sitios web de los seis medios de comunicación rumanos más visibles. Este estudio contribuye al conocimiento existente sobre las representaciones mediáticas de los migrantes rumanos al documentar una serie de tendencias, incluyendo un enfoque orientado a los acontecimientos, representaciones excesivamente simplificadas de la migración, masificación y esquematización de las representaciones de los migrantes, y la alta sensibilidad a los informes de los medios de comunicación de los países de destino sobre los migrantes rumanos. Nuestro análisis revela que el enfoque adoptado para informar sobre la migración durante la pandemia del COVID-19, al menos durante su primera fase, dependió en gran medida de los modos existentes e institucionalizados de informar sobre la migración en los medios de comunicación.

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Generation and cryopreservation of feline oviductal organoids

Cited by 3 publications

References 31 publications

The porcine islet-derived organoid showed the characteristics as pancreatic duct

The porcine islet-derived organoid showed the characteristics as pancreatic duct

Bovine oviductal organoids: a multi-omics approach to capture the cellular and extracellular molecular response of the oviduct to heat stress

La pandemia de COVID-19 como oportunidad para poner de relieve la migración de los trabajadores agrícolas a través de la cobertura mediática

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