Generation and characterization of mouse microglial cell lines

Briers, T.; Desmaretz, Christel; Vanmechelen, Eugeen

doi:10.1016/0165-5728(94)90109-0

Cited by 36 publications

(19 citation statements)

References 50 publications

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“…Various hypotheses suggest that the process of cavernous degeneration is related to lysozyme activity (Kao et al, 1977), pulsatile hydrodynamics (Williams et al, 1981), inflammation, macrophage activation, and subsequent astrocyte migration (Fitch et al, 1999). Microglia, the intrinsic brain macrophages, secrete EGF after CNS injury (Briers et al, 1994;Nolte et al, 1997). Extrapolating our in vitro findings to in vivo, activation of the EGFR pathway in the reactive astrocytes may signal the progressive formation of cavernous spaces in CNS injuries.…”

Section: Discussionmentioning

confidence: 85%

Activation of epidermal growth factor receptor causes astrocytes to form cribriform structures

Liu

Neufeld

2004

Glia

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Epidermal growth factor receptor (EGFR) is expressed in reactive astrocytes following injury in the CNS. However, the effects of activation of the EGFR pathway in astrocytes are not well established. In the present study, we demonstrate that activation of EGFR causes optic nerve astrocytes, as well as brain astrocytes, to form cribriform structures with cavernous spaces. Formation of the cribriform structures is dependent on new protein synthesis and cell proliferation. Platelet-derived growth factor and basic fibroblast growth factor were not effective. Smooth muscle cells and epithelial cells do not form cribriform structures in response to EGFR activation. The formation of the cribriform structures appears to be related to a guided migration of astrocytes and the expression of integrin beta1 and extracellular fibronectin in response to activation of EGFR. The EGFR pathway may be a specific, signal transduction pathway that regulates reactive astrocytes to form cavernous spaces in the glial scars following CNS injury and in the compressed optic nerve in glaucomatous optic nerve neuropathy.

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Section: Discussionmentioning

confidence: 85%

Activation of epidermal growth factor receptor causes astrocytes to form cribriform structures

Liu

Neufeld

2004

Glia

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“…There have been several reports describing the establishment of microglia cell lines, generated by infection with retrovirus constructs carrying v-raf/v-myc (Blasi (Righi et al, 1989) or by transfection with simian virus 40 large T (Janabi et al, 1995;Hosaka et al, 1992;McLaurin et al, 1995) or the v-myc gene (Briers et al, 1994). However, most of these cells were transformed by these oncogenes, and therefore tended to lose some of the characteristic properties of primary microglia.…”

Section: Discussionmentioning

confidence: 98%

“…One approach to overcoming this problem is to use microglial cell lines that retain the phenotype of primary-cultured microglia. Some groups of investigators, including our own, have been trying to establish a microglial cell line by transformations with viral or cellular oncogenes (Righi et al, 1989;Blasi et al, 1990;Hosaka et al, 1992;Briers et al, 1994;Janabi et al, 1995;McLaurin et al, 1995). However, most of the transformed cells lost some of the natural characteristic properties of microglia.…”

Section: Introductionmentioning

confidence: 99%

Generation and characterization of a microglial cell line, MG5, derived from a p53-deficient mouse

Ohsawa

Imai

Nakajima

et al. 1997

Glia

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“…They secrete cytokines and growth factors that stimulate the proliferation of numerous cell types, including some CNS glia (reviewed in Hamilton et al, 1993;Turpin and LopezBerestein, 1993). Macrophages secrete several kinds of cytokines that are thought to be involved in macrophagemediated cellular proliferation, including transforming growth factor-␣ (TGF-␣), epidermal growth factor (EGF), heparin-binding EGF-like growth factor (HB-EGF), insulin-like growth factor-I (IGF-I), basic fibroblast growth factor (FGF-2), transforming growth factor-␤ (TGF-␤), and interleukin-1 (Baird et al, 1985;Assoian et al, 1987;Madtes et al, 1988;Rappolee et al, 1988;Rom et al, 1988;Platanias and Vogelzang, 1990;Higashiyama et al, 1991;Briers et al, 1994). Several of these factors (EGF, TGF-␣, and IGF-I) are mitogenic for adult inner ear vestibular SE (reviewed in Oesterle and Hume, 1999).…”

Section: Benefits Of Leukocyte Subtype Identification In Inner Ear Sementioning

confidence: 99%

Characterization of leukocyte subtypes in chicken inner ear sensory epithelia

O'Halloran

Oesterle

2004

J of Comparative Neurology

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Human hearing and balance require intact inner ear sensory hair cells, which transduce mechanical stimuli into electrical signals that are transmitted to the brain. Loss of hair cells after birth in mammals is irreversible, whereas birds are able to regenerate hair cells after insult and demonstrate ongoing hair cell production in the vestibular epithelia. Leukocytes reside in undamaged sensory epithelia of the avian inner ear and increase in number after trauma, prior to the proliferation of hair cell progenitors. It has been hypothesized that leukocyte-produced growth factors or cytokines may be involved in triggering hair cell regeneration. Little is known about the specific leukocyte subtypes present in avian ear. Immunohistochemistry with a panel of monoclonal antibodies to chicken leukocytes was used to identify leukocyte subtypes in normal posthatch chicken ear sensory epithelia. The responsiveness of the leukocytes to aminoglycoside-induced damage was also observed. Based on immunocytochemical and morphological criteria, we quantified leukocyte subtypes in normal and drug-damaged auditory and vestibular sensory epithelia. Data indicate that lymphocytes (B and T cells) do not reside in normal or drug-damaged ear sensory epithelia at 1-3 days post insult but are present in adjacent nonsensory tissues. The most common leukocytes in inner ear sensory epithelia are ramified cells of the myeloid lineage. Many of these are MHC class II positive, and a small percentage are mature tissue macrophages. An absence of leukocytes in lesioned areas of the auditory sensory epithelium suggests they may not play a critical role in triggering hair cell regeneration.

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Generation and characterization of mouse microglial cell lines

Cited by 36 publications

References 50 publications

Activation of epidermal growth factor receptor causes astrocytes to form cribriform structures

Activation of epidermal growth factor receptor causes astrocytes to form cribriform structures

Generation and characterization of a microglial cell line, MG5, derived from a p53-deficient mouse

Characterization of leukocyte subtypes in chicken inner ear sensory epithelia

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