Generation and characterization of an immune combinatorial cDNA library of variable genes of mouse immunoglobulins

Pavlova, M. V.; Gilchuk, Pavlo; Pokholenko, Ianina; Nikolaiev, I. S.; Кордюм, В. А.

doi:10.1007/s11956-008-2002-4

Cited by 4 publications

(2 citation statements)

References 8 publications

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“…Fortunately, combinatorial molecular biology can allow us to overcome this significant hurdle. Combinatorial antibody libraries are libraries of antibody heavy and light chain genes that are isolated from B cells derived from naïve, immunized 6,7 or infected 8,9 subjects or are either partially 10 or fully 11 synthesized chemically in vitro. The diversity of the combinatorial libraries can exceed that of the natural opposed to a two (or more) site sandwich type immunometric platform, thereby reducing overall assay complexity.…”

Section: Introductionmentioning

confidence: 99%

Exploiting recombinant antibodies in point-of-care (POC) diagnostics: The combinatorial advantage

Hearty

O’Kennedy²

2011

Bioengineered Bugs

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Antibodies are ubiquitously deployed on in vitro diagnostic (IVD) platforms for detecting a panoply of analytes indicative of environmental and food contamination, residue adulteration and both veterinary and medical diagnostics. In the clinical realm, rapid and accurate determination of disease status is paramount. The significance of immunodiagnostic performance cannot be overemphasized and in many cases reliable diagnosis informs medical intervention which can mean the difference between patient recovery and demise. Cardiovascular disease (CVD) is the single biggest cause of adult mortality in the western world and principal burden on the healthcare services. Although the troponin (Tn) family, in particular troponin I (TnI), are regarded as the gold standard for diagnosis of myocardial damage, over the last decade much research has focused on the identification of alternative cardiac biomarker molecules that can either supplant or complement TnI metrics to add value to cardiac risk stratification criteria. In particular, markers that appear earlier than TnI in the pathophyisiology of cardiac disease are highly sought after. The subject of this addendum represents part of a broader challenge to deliver novel rapid point-of-care (POC) diagnostics to provide a chip-based multi-plexed platform for more comprehensive profiling of cardiac status with additive diagnostic and prognostic value. Specifically, it outlines proof-of-concept direct myeloperoxidase (MPO) detection, demonstrates the benefits of using recombinant antibodies in POC diagnostics and describes optimized strategies for generation of superior candidate antibody panels.

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Section: Introductionmentioning

confidence: 99%

Exploiting recombinant antibodies in point-of-care (POC) diagnostics: The combinatorial advantage

Hearty

O’Kennedy²

2011

Bioengineered Bugs

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“…As for the precise technological support (purification, testing, delivery, etc. ), we set up and are developing the technology of recombinant single-strand monoclonal antibodies [45][46][47]. Now we started uniting all these to obtain the «inter…»effects -the elaboration of technologies of applying genetically modified cells on the background of cytokine field in the damaged part of the organism to reconstruct the desired using stem cells of the same organism.…”

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confidence: 99%

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2013

Biopolym. Cell

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The article-generalization deals with the main directions being developed in the Department of Cell Regulatory Mechanisms during past 40 years from the moment of the Institute of Molecular Biology and Genetics of Academy of Sciences of Ukraine organization up to our days. The main steps of space and molecular biology in the USSR and future development of biology in Ukraine are presented in this review.

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In silico analysis of the structure of variable domains of mouse single-chain antibodies specific to the human recombinant interferon β1b

2009

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Generation and characterization of an immune combinatorial cDNA library of variable genes of mouse immunoglobulins

Cited by 4 publications

References 8 publications

Exploiting recombinant antibodies in point-of-care (POC) diagnostics: The combinatorial advantage

Exploiting recombinant antibodies in point-of-care (POC) diagnostics: The combinatorial advantage

Untitled

In silico analysis of the structure of variable domains of mouse single-chain antibodies specific to the human recombinant interferon β1b

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