Generation and Characterisation of Monoclonal Antibodies to Oleanolic Acid

Abstract

Monoclonal antibodies to oleanolic acid, a pentacyclic triterpene, were generated using the hybridoma fusion method described by Kohler and Milstein . Protein conjugates of the target molecule for immunisation were prepared either by directly linking the isoprenoid to different protein carriers via the carboxylic function or after introduction of a succinic spacer between the protein carrier and position 3 of the target molecule. Antibodies of three different cell lines were further analysed by competitive ELISA and were shown to be directed either against both rings A and B or to ring E of the pentacyclic system. Thus these antibodies can be used in the specific detection of oleanolic acid itself or of a broad range of oleanolic acid derivatives sharing a specific structural moiety. Therefore these antibodies may be interesting tools for the screening of putative terpenoid-containing plants.

Birch bark research and development

“…[131][132][133] The following pharmacological activities of oleanolic acid (5) and derivatives also merit special mention: prevention and treatment of anxiety and depression in mammals; 134 treatment of hyper-sensitivity and/or hyper-reactivity, 135 and treatment of non-insulin-dependent diabetes mellitus. 136 In addition, they have been found to promote antibody generation 137 and immunomodulatory activity, 138 function as vasodilators and restorative agents for endothelial dysfunction, 139 and exert gastroprotective effects. 140 The use of oleanoic acid (5) as a component of complementary and conventional medicines is common in China.…”