Generation and application of a novel transgenic zebrafish line Tg(cyp1a:mCherry) as an in vivo assay to sensitively monitor PAHs and TCDD in the environment

Xie, Shaolin; Junaid, Muhammad; Bian, Wan-Ping; Luo, Juanjuan; Syed, Jabir Hussain; Wang, Chao; Xiong, Wen-Xu; Ma, Yanbo; Niu, Aping; Yang, Xiaojun; Zou, Jixing; Pei, De‐Sheng

doi:10.1016/j.jhazmat.2017.11.021

Cited by 28 publications

(15 citation statements)

References 45 publications

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“…AhR is abundant in the cytoplasm, is transported to the nucleus by heterodimerization with ARNT and then binds to the xenobiotic response element (XRE) at the promoter of the gene, thereby activating gene expression ( Whitelaw et al, 1993 ). Many exogenous compounds, such as polycyclic aromatic hydrocarbons (PAHs), biphenyls (PCBs), and halogenated aromatic hydrocarbons (HAHs), induce CYP1A1 expression by AhR-dependent pathways ( Hanlon et al, 2005 ; Fazili et al, 2010 ; Xie et al, 2018 ). However, several studies have found that AhR-independent pathways regulate the induction of CYP1A1 .…”

Section: Introductionmentioning

confidence: 99%

Sp1, Instead of AhR, Regulates the Basal Transcription of Porcine CYP1A1 at the Proximal Promoter

Xie

Jiang

et al. 2018

Front. Pharmacol.

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Pigs are commonly used as an animal model to evaluate the toxic effects of exogenous compounds. Cytochrome P450 1A1 (CYP1A1) metabolizes numerous exogenous compounds and is abundantly expressed in the liver, kidneys, and intestines. The high amino acid similarity between human and porcine CYP1A1 indicates that they probably have the same metabolic characteristics. Therefore, understanding the regulatory mechanism of CYP1A1 expression in pigs is particularly important for predicting the toxicology and metabolic kinetics of exogenous chemicals. Currently, the transcriptional regulation of porcine CYP1A1 has rarely been studied, especially regarding basal transcription. In this study, we first confirmed that the key regulatory elements of porcine CYP1A1 basal transactivation are in the proximal promoter region using promoter truncation analysis via a dual luciferase assay in a porcine kidney cell line LLC-PK1. Two overlapping cis-elements, the xenobiotic response element (XRE) and GC box, in this proximal region potentially play key roles in the basal transactivation of porcine CYP1A1. Furthermore, using electrophoretic mobility shift assay and chromatin immunoprecipitation, the GC box binding protein Sp1 was confirmed to bind to the proximal promoter of porcine CYP1A1, instead of AhR, the XRE binding protein. In LLC-PK1 cells, by knocking down either Sp1 or AhR, the expression of porcine CYP1A1 at the mRNA level and protein level was significantly downregulated, suggesting both proteins are important for porcine CYP1A1 expression. However, promoter activity analysis in LLC-PK1 cells treated with an AhR agonist and antagonist confirmed that AhR does not participate in the basal regulation of porcine CYP1A1 at the proximal promoter. In conclusion, our study revealed that the proximal promoter is the key regulatory region for porcine CYP1A1 basal expression. Although AhR plays an important role in the transactivation of porcine CYP1A1 expression, the key determinant transcription factor for its basal transactivation is Sp1 at the proximal promoter of porcine CYP1A1.

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Section: Introductionmentioning

confidence: 99%

Sp1, Instead of AhR, Regulates the Basal Transcription of Porcine CYP1A1 at the Proximal Promoter

Xie

Jiang

et al. 2018

Front. Pharmacol.

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“…Zebrafish is the most widely used animal model because of the availability of mature individuals with stable inheritance within three months (Dai et al 2014;Luo et al 2018;Xie et al 2018). A transgenic zebrafish line, Tg (4xE-box: Luc), was established for the studying the biological clock and developmental regulation of the vertebrate brain through expressing a luciferase reporter gene under the control of a minimal promoter and four E-boxes (Weger et al 2013).…”

Section: Genetic Model Of Developmentmentioning

confidence: 99%

“…2018; Xie et al . 2018). A transgenic zebrafish line, Tg (4xE‐box: Luc), was established for the studying the biological clock and developmental regulation of the vertebrate brain through expressing a luciferase reporter gene under the control of a minimal promoter and four E‐boxes (Weger et al .…”

Section: Genetically Modified Fish Applicationsmentioning

confidence: 99%

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A comprehensive review on genetically modified fish: key techniques, applications and future prospects

Wang

Hamid

Jia

et al. 2021

Reviews in Aquaculture

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Genetically modified fish is a general term used for whole fish whose DNA has been artificially altered by directly integrating (or deleting) single or multiple genes to introduce or modify a targeted character. Different techniques have been performed to produce genetically modified fish. Importantly, the introduction of various types of fluorescent proteins has dramatically expanded the range of applications of genetically modified fish. Currently, genetically modified fish have been widely employed as experimental models for medical science, pharmacology and environmental toxicology. The commercial application of genetically modified fish in aquaculture is still up for debate, although the first commercialization of genetically modified fish, GloFish, was in 2003, and further, the United States and Canada approved the commercial production and sale of the AquAdvantage Salmon. In this review, we discuss the principles and developmental history of fish transgenesis via gene transfer and gene-editing techniques, and their applications in diverse fields. Further, challenges and prospects associated with the genetically modified fish are discussed.

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“…PAHs commonly plays an important role through Aryl hydrocarbon Receptor (AhR) pathway and alters the expression levels of CYP450 family enzymes (Staal et al, 2006;Xie et al, 2017). However, the Low Molecular Weight (LMW) PAHs (3-and 4-ring), such as Fluorene (Flu), Acenaphthene (Ace), Acenaphthylene (Acp) and Phe, showed no affinity to AhR (Xu et al, 2015).…”

Section: Ecological Risks Of Pahs Dehp and Ppcpsmentioning

confidence: 99%

Environmental Concerns and Toxicogenetic Endpoints of Priority Substances (PSs) and Contaminants of Emerging Concerns (CECs): A Comprehensive Review

Liu¹,

Junaid²,

Hamid³

et al. 2018

American Journal of Environmental Sciences

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Priority Substance (PSs) and Contaminant of Emerging Concerns (CECs) exhibited a wide range of environmental and public health concerns worldwide. This review summarized the documented studies related to the current surface water occurrence, spatial distribution, ecological risks and toxicity of selected PSs, such as polycyclic aromatic hydrocarbons (PAHs) and CECs, such as Di(2-Ethylhexyl) Phthalate (DEHP) and Pharmaceuticals and Personal Care Products (PPCPs). The spatial distribution analysis revealed alarming levels of PAHs in the surface waters of Asian counties, e.g., 84210 ng L −1 in Gomti River, India, 29325 ng L −1 in Daya Bay, China and 1287 ng L −1 in Chenab River, Pakistan. As for DEHP, the highest concentrations of 13050 µg L −1 in Liao River, China, and 2306 µg L −1 in Rivers of Eastern Cape, South Africa were reported. These environmental levels of PAHs and DEHP were many folds higher than the surface water permissible levels devised by WHO and USEPA. Contrarily, the emerging PPCPs were reported in relatively lower levels in the surface waters globally, compared to that of PAHs and DEHP. Consistent with the environmental levels, PAHs and DEHP revealed alarming ecological risks in the surface water sources, compared to that of PPCPs. Regarding to the sources of PSs and CECs, PAHs emissions were mostly linked to the incomplete combustion of petroleum products, DEHP contamination was associated to its applications in consumption and production of plastic appliances and PPCPs emissions were largely related to the domestic and industrial effluents. As for toxic endpoints of PAHs, DEHP and PPCPs, all of these were reported to cause DNA damage, genotoxicity, reproductive toxicity, developmental toxicity and immunotoxicity, as revealed in reviewed in vitro/vivo studies. In addition, the current review also highlighted the existing environmental regulations to control the emissions of these pollutants to the environmental matrices. Taken together, this review concluded that despite the existing environmental regulations, the current levels of organic pollutants are still on rising, especially in Asian countries. Therefore, the strict implementation of the existing regulations is highly necessary to control these pollutants to ensure public health and ecological integrity.

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Generation and application of a novel transgenic zebrafish line Tg(cyp1a:mCherry) as an in vivo assay to sensitively monitor PAHs and TCDD in the environment

Cited by 28 publications

References 45 publications

Sp1, Instead of AhR, Regulates the Basal Transcription of Porcine CYP1A1 at the Proximal Promoter

Sp1, Instead of AhR, Regulates the Basal Transcription of Porcine CYP1A1 at the Proximal Promoter

A comprehensive review on genetically modified fish: key techniques, applications and future prospects

Environmental Concerns and Toxicogenetic Endpoints of Priority Substances (PSs) and Contaminants of Emerging Concerns (CECs): A Comprehensive Review

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