Generation and analysis of expressed sequence tags from the mangrove plant, Acanthus ebracteatus Vahl

Nguyen, Phuoc Dang; Ho, Chai Ling; Harikrishna, Jennifer Ann; Wong, Clemente Michael Vui Ling; Rahim, Raha Abdul

doi:10.1007/s11295-006-0044-2

Cited by 22 publications

(18 citation statements)

References 43 publications

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“…The response of mangroves to salt stress has mainly been studied in leaves (Banzai et al 2002;Fu et al 2005;Mehta et al 2005;Nguyen et al 2006;and others), and little data have been obtained on salt stress-dependent changes in the gene expression of root tissues (Wong et al 2007;Ezawa and Tada 2009). Root tissues are in contact with salt and are the primary site involved in the perception of salinity stress; as such, they are assumed to have a remarkable ability to control their Na + and Cl − content.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Salt Stress Tolerance Genes from Roots of Mangrove Plant, Rhizophora stylosa Griff., Using PCR-Based Suppression Subtractive Hybridization

et al. 2010

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Salinity is a major abiotic stress that seriously limits plant growth and crop productivity. The halophytic Rhizophora stylosa is useful for the study of the molecular mechanisms behind salinity tolerance in mangrove trees. To isolate anti-salt stress genes from mangrove plants, a cDNA library of R. stylosa roots was constructed and screened for stress-related genes by polymerase chain reaction (PCR)-based suppressive subtractive hybridization (SSH). The mangrove seedlings were grown for 8 months under two conditions: exposure to water with 3% salt and exposure to fresh water. cDNA of seedlings exposed to water with 3% salt was used as a tester and cDNA for freshwater germination was used as a driver. We isolated and sequenced 240 up-regulated expressed sequence tags (ESTs) from the SSH library. Among these up-regulated ESTs, 48 unique clones were putatively identified and classified into ten functional categories, such as cell rescue and defense, secondary metabolism, protein synthesis, and metabolism. Fifteen genes from different categories were selected and their expression was studied by real-time RT-PCR. Significantly increased expression levels were confirmed for 13 of these 15 transcripts, which suggest that these genes contribute to the salt tolerance of this plant. Among them, two transcription factors and several genes involved in isoprenoid biosynthesis were identified from mangrove trees as salt tolerance genes for the first time. The physiological significance of the increased expression of these genes in the long-term adaptation of mangrove trees to salt stress is discussed.

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Section: Introductionmentioning

confidence: 99%

Isolation of Salt Stress Tolerance Genes from Roots of Mangrove Plant, Rhizophora stylosa Griff., Using PCR-Based Suppression Subtractive Hybridization

et al. 2010

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“…While, the fruit of A. ebracteatus utilized as a blood purifier, leaf paste is applied in a dressing of burns, roots in snakebite and also used in pain caused by wounds and the seeds are used as anthelminthic [36]. 170 genes of A. ebracteatus are recognized which are engrossed in response to salt stress [37].…”

Section: Traditional Usesmentioning

confidence: 99%

Ethnomedicinal, Phytochemical, and Pharmacological Aspects of Genus Acanthus

Bora

Adhikari

Das

et al. 2017

Int J Pharm Pharm Sci

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Acanthus (family Acanthaceae) is a genus of the major group angiosperms (flowering plants) comprising more than 29 species widely distributed in the tropical and subtropical region. The aim of this review is to offer thorough scientific information on ethnomedicinal uses, phytochemical, and pharmacological activities of the plant species belonging to the genus Acanthus. Numerous traditional uses of the genus have been supported in this article from the results, and executed by the extracts and/or pure compounds obtained from pharmacological studies will provide a single platform to help future researches on the genus Acanthus. Furthermore, ethnomedicinal evaluation using fractions or isolation of medicinal principles was extensively explored by databases like Google, Google Scholar, and Science Direct at the same time.

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“…It has been reported that a moderate decrease in plastid aldolase activity inhibits photosynthesis, alters the level of sugars and starch and inhibits the growth of potato plants (Haake et al 1998). The chloroplastic FBP gene is differentially expressed under temperature stress (Michelis and Gepstein 2000), herbivore attack (Voelckel and Baldwin 2003), salt stress (Zorb et al 2004;Nguyen et al 2006) and in Selenium metabolism (Agalou et al 2006). The observations of over expression of this gene under biotic and abiotic stresses make it a strong candidate for study under drought stress.…”

Section: Mots Clé Smentioning

confidence: 99%

Identification, sequencing and characterization of a stress induced homologue of fructose bisphosphate aldolase from cotton

Qaisar¹,

Irfan²,

Meqbool³

et al. 2010

Can. J. Plant Sci.

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, T. 2010. Identification, sequencing and characterization of a stress induced homologue of fructose bisphosphate aldolase from cotton. Can. J. Plant Sci. 90: 41Á48. A drought-induced homologue of fructose-1, 6 bisphosphate aldolase full length cDNA (GaAldp) was isolated and sequenced by gene homology and rapid amplification of cDNA ends (RACE) from cotton variety FDH-786 (Gossypium arboretum). Quantitative polymerase chain reaction (PCR) analysis showed that drought stress, salinity and exogenous treatment with abscisic acid (ABA) enhanced the accumulation of GaAldp transcripts in the leaf and stem tissues of the plant. An alignment of the 1413 bp cDNA and 1937 bp genomic DNA sequences revealed that GaAldp has an open reading frame (ORF) encoding 394 amino acids and contains five introns. The predicted amino acid sequence is homologous to a heat-induced isoform of oat chlorplastic fructose bisphosphate aldolase (FBP) (AF216582) and NPALDP1 from Nicotiana paniculata. The GaAldp sequence includes a novel stroma targeting N-terminal transit peptide (TP) of 45 amino acids, which is 63Á76% similar to other chloroplastic TPs.

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Generation and analysis of expressed sequence tags from the mangrove plant, Acanthus ebracteatus Vahl

Cited by 22 publications

References 43 publications

Isolation of Salt Stress Tolerance Genes from Roots of Mangrove Plant, Rhizophora stylosa Griff., Using PCR-Based Suppression Subtractive Hybridization

Isolation of Salt Stress Tolerance Genes from Roots of Mangrove Plant, Rhizophora stylosa Griff., Using PCR-Based Suppression Subtractive Hybridization

Ethnomedicinal, Phytochemical, and Pharmacological Aspects of Genus Acanthus

Identification, sequencing and characterization of a stress induced homologue of fructose bisphosphate aldolase from cotton

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