Generation and analysis of expressed sequence tags from the salt-tolerant mangrove species Avicennia marina (Forsk) Vierh.

Abstract

Salinization poses an increasingly serious problem in coastal and agricultural areas with negative effects on plant productivity and yield. Avicennia marina is a pantropical mangrove species that can survive in highly saline conditions. As a first step towards the characterization of genes that contribute to combating salinity stress, the construction of a cDNA library of A. marina genes is reported here. Random expressed sequence tag (EST) sequencing of 1,841 clones produced 1,602 quality reads. These clones were classified into functional categories, and BLAST: comparisons revealed that 113 clones were homologous to genes earlier implicated in stress responses, of which the dehydrins are the most predominant in this category. Of the ESTs analyzed, 30% showed homology to previously uncharacterized genes in the public plant databases. Of these 30%, 52 clones were selected for reverse Northern analysis: 26 were shown to be up-regulated and five shown to be down-regulated. The results obtained by reverse Northern analysis were confirmed by Northern analysis for three clones.

Construction of a cDNA Library from the Ephemeral Plant Olimarabidopsis pumila and Preliminary Analysis of Expressed Sequence Tags

“…ESTs have proven to be an effi cient, rapid means to identify novel genes involved in tolerance to environmental stress (Jha et al, 2009;Nishiuchi et al, 2010). Large-scale cDNA sequencing and EST analyses have been successfully used to identify stress tolerance genes in a large number of plants, such as Suaeda salsa (Zhang et al, 2001), Avicennia marina (Mehta et al, 2005), Thellungiella halophila (Du et al, 2008;Taji et al, 2008), Salicornia brachiata (Jha et al, 2009), and Suaeda asparagoides (Ayarpadikannan et al, 2012).…”

Identification of salt-induced genes from Salicornia brachiata, an extreme halophyte through expressed sequence tags analysis

“…Various researchers have recently reported genes and ESTs, induced by cold, drought, high salinity stress and ABA in many plant species (Amtmann et al, 2005;Vinocur and Altman, 2005;Xu et al, 2007). There are few reports on ESTs generation and analysis in response to salt treatment on different halophytes like Suaeda salsa (Zhang et al, 2001), Tamarix androssowii (Wang et al, 2006), Thellungiella halophila (Wang et al, 2004), Mesembryanthemum crystallinum (Kore-eda et al, 2004) and Avicennia marina (Mehta et al, 2005).…”

Strategies and models for agricultural sustainability in developing Asian countries

“…The MSSRF scientists have accordingly incorporated the salinity-tolerance genes from a mangrove species, Avicennia marina into rice. The transgenic rice under field trials is able to tolerate up to 150 mM of salt-induced stress (Mehta et al 2005; (ii) Green agriculture: cultivation with the help of integrated pest management, integrated nutrient supply and integrated natural resource management systems. (iii) Lodging resistance (iii) Ecoagriculture: based on conservation of soil, water and biodiversity and the application of traditional knowledge and ecological prudence (iv) EM agriculture: system of farming using effective microorganisms (EMs) (v) White agriculture: system of agriculture based on substantial use of microorganisms, particularly fungi (vi) One-straw revolution: system of natural farming without ploughing, chemical fertilizers, weeding and chemical pesticides and herbicides Prashant & Parida 2005).…”