Generation and analysis of drought stressed subtracted expressed sequence tags from safflower (Carthamus tinctorius L.)

Thippeswamy, M.; Sivakumar, M.; Sudhakarbabu, O.; Reddy, P. Chandraobul; Veeranagamallaiah, G.; Pandurangaiah, Merum; Ramya, Mummadireddy; Nareshkumar, Ambekar; Kirankumar, T.; Sudhakar, Chinta

doi:10.1007/s10725-012-9744-9

Cited by 8 publications

(4 citation statements)

References 49 publications

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“…For instance, Thippeswamy et al (2013) differentially evaluated two safflower cultivars (A1 and Nira) and identified cultivar A1 as relatively drought tolerant. In order to recognize drought-responsive genes, they constructed a subtracted cDNA library from cultivar A1 and identified 667 unique drought-responsive ESTs.…”

Section: Functional Genomics For Drought Tolerancementioning

confidence: 99%

“…Remarkably, even though that majority of them had significant homology to other plants, more than 20 % of drought-induced ESTs were not homologous to any sequences in databases and were considered novel drought-responsive genes of safflower. Insight into the function of these genes and demonstration of their novelty may pilot to a better perception of the drought tolerance mechanisms in safflower and potentially other oilseed crops (Thippeswamy et al 2013). Lulin et al (2012) also conducted large-scale genomics, in order to investigate genes and pathways that may regulate flavonoids, the biosynthesis of unsaturated fatty acids (that can be beneficial to oxidative stress caused by abiotic stresses like drought), and other secondary metabolites in the safflower.…”

Section: Functional Genomics For Drought Tolerancementioning

confidence: 99%

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Salt and drought stresses in safflower: a review

Hussain

Lyra

Farooq

et al. 2015

Agron. Sustain. Dev.

188

130

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Safflower is one of the oldest cultivated crops, usually grown at a small scale. Safflower is grown for flowers used for coloring, flavoring foods, dyes, medicinal properties, and livestock feed. Safflower is underutilized but gaining attention due to oil yield potential and the ability to grow under high temperatures, drought, and salinity. Salinity and drought have negative effects by disrupting the ionic and osmotic equilibrium of the plant cells. The stress signal is perceived by membranes then transduced in the cell to switch on the stress responsive genes. This review discusses on stress tolerance mechanisms in safflower. Strategies are proposed for enhancing drought and salt resistance in safflower.

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Section: Functional Genomics For Drought Tolerancementioning

confidence: 99%

Section: Functional Genomics For Drought Tolerancementioning

confidence: 99%

Salt and drought stresses in safflower: a review

Hussain

Lyra

Farooq

et al. 2015

Agron. Sustain. Dev.

188

130

View full text Add to dashboard Cite

show abstract

“…EST-SSR containing pentanucleotide repeat (GAAGT) matched with myo-inositol 1-phosphate synthase protein which was shown to play an important role in drought tolerance in chickpea [35]. Expression of MIPS2 was observed in different seed developmental stages in response to heat stress in rice and Arabidopsis [36][37][38]. In particular, interference of SSRs with S-adenosylmethionine synthetase, one-helix protein and myoinositol 1-phosphate synthetase proteins play important roles in plant response to drought stress.…”

Section: In -Silico Pcr Results and Functional Annotation Of Est-ssrsmentioning

confidence: 99%

In silico Mining of EST-SSRs from the Drought Tolerant ESTs in Safflower

Sudhakar¹,

Thippeswamy²,

Sivakumar³

et al. 2019

J Proteomics Bioinform

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Drought stress is a major abiotic factor causing yield loss in safflower and limited studies have been carried out to dissect the molecular mechanism at EST-SSR level. In this study, a possible relationship between simple sequence repeats (SSRs) distribution and drought stress was studied using three EST libraries of safflower, Ct-D-EST, Ct-NEST and Co-EST. The Ct-D-EST was generated from drought tolerant safflower cultivar A-1; Ct-NEST and Co-EST were the EST datasets of safflower and its wild progenitor C. oxycanthus, respectively. In total, 156 (45%), 1194 (5%) and 1550 (9%) EST-SSRs were mined from Ct-D-EST, Ct-NEST and Co-EST libraries, respectively. Comparison of EST-SSRs from Ct-D-EST with that of SSRs from other two libraries showed reasonable differences for each class of repeats. Large variations were observed for dinucleotide repeats in all the libraries. In drought EST-SSRs, only one kind of amino acid was produced by repeat (ATG) 14 which encodes met indicating the loci and repeat observed to be 100%. Since three ESTs with SSRs from Ct-D-EST, annotated to putative candidate genes, S-adenosylmethionine synthetase, one-helix protein and myo-inositol 1-phosphate synthetase, did not express with SSRs in the Ct-NEST , these can be considered as potential candidate genes for drought tolerance. A trinuceotinde SSR, encoding met, was also found in the EST annotated to putative s-adenosylmethionine synthetase, hence, both can be considered as indices for drought tolerance. The genomic resources and the information generated in this study may be useful for the safflower breeders particularly for the development of drought tolerant cultivar.

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“…Safflower ( Carthamus tinctorius L.), from the Asteraceae family, is an annual herbaceous species, which has been targeted for its potential in large‐scale production of high quality edible oil (Sabzalian et al, ). Although safflower is grown throughout semi‐arid regions (Thippeswamy et al, ), its genetic resources have not been extensively described and assessed for drought tolerance capacity yet. This is especially the case in Algeria where two related wild species of safflower can be found ( Carthamus strictus Batt.…”

Section: Different Classifications In Carthamus Slmentioning

confidence: 99%

Phylogenetic position of two endemic Carthamus species in Algeria and their potential as sources of genes for water use efficiency improvement of safflower

Mihoub

Robert

Ghashghaie

et al. 2016

J of Sytematics Evolution

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Despite the high economic value of safflower (Carthamus tinctorius), the potential of several wild related species as genetic resources is still poorly studied, in particular with regards to drought tolerance, a priority in safflower improvement. This situation is partially explained by the numerous and still discussed uncertainties in phylogenetic relationships within the safflower species complex. In this study, we focused on two rare Algerian wild species, Carthamus strictus and Carthamus chouletteanus. We aimed at: (i) clarifying their taxonomic status, and determining their phylogenetic relationships with safflower and other Mediterranean species of Carthamus sensu lato (s.l.) (Carthamus sensu stricto (s.s.) and Carduncellus genera) by using nucleotide diversity within the internal transcribed spacer region (ITS) and (ii) assessing the water use efficiency (WUE) of these species by determining their carbon isotope composition. Our results support the existence of two well supported different clusters among the safflower species complex: Carthamus s.s. including Eastern annual species and the vast Carduncellus group constituted by Western perennial species, including the two studied Algerian species. Also, significant differences for carbon isotopic composition between Carthamus s.s. and Carduncellus were revealed. Carduncellus species displayed significantly higher d 13 C values (i.e., higher WUE) than Carthamus s.s. species. Carthamus chouletteanus displayed the highest average value of d 13 C (highest WUE) among the species studied. Our data encourage for further studies on the possibility of hybridisations between species of the two genera and suggest that Carduncellus should be considered as potentially more interesting genetic resources to improve the WUE of safflower, rather than Carthamus s.s. species.

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Generation and analysis of drought stressed subtracted expressed sequence tags from safflower (Carthamus tinctorius L.)

Cited by 8 publications

References 49 publications

Salt and drought stresses in safflower: a review

Salt and drought stresses in safflower: a review

In silico Mining of EST-SSRs from the Drought Tolerant ESTs in Safflower

Phylogenetic position of two endemic Carthamus species in Algeria and their potential as sources of genes for water use efficiency improvement of safflower

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