Generation, analysis and functional annotation of expressed sequence tags from the ectoparasitic mite Psoroptes ovis

Burgess, Stewart T. G.; Nisbet, Alasdair J.; Kenyon, Fiona; Huntley, John F.

doi:10.1186/1756-3305-4-145

Cited by 12 publications

(12 citation statements)

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“…To further expand knowledge of the P. ovis transcriptome, a cDNA library of P. ovis was constructed by Burgess et al [20]; 1,574 ESTs were sequenced and, combined with 484 previously obtained P. ovis sequences such that, resulted in 1,545 mite sequences. Li et al sequenced 33 P. ovis var.…”

Section: Discussionmentioning

confidence: 99%

“…cDNA libraries of P. ovis were constructed by Kenyon et al [45] and Burgess et al [20], and a cDNA library of P. ovis var. cuniculi was constructed by Hu et al[46] .…”

Section: Discussionmentioning

confidence: 99%

“…However, a lack of available sequence information hinders progress in these areas. This far, only 1,545 unique P. ovis expressed sequence tags (ESTs) have been identified [20]; there is no available reference genome.…”

Section: Introductionmentioning

confidence: 99%

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Preliminary analysis of Psoroptes ovis transcriptome in different developmental stages

et al. 2016

Parasites Vectors

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BackgroundPsoroptic mange is a chronic, refractory, contagious and infectious disease mainly caused by the mange mite Psoroptes ovis, which can infect horses, sheep, buffaloes, rabbits, other domestic animals, deer, wild camels, foxes, minks, lemurs, alpacas, elks and other wild animals. Features of the disease include intense pruritus and dermatitis, depilation and hyperkeratosis, which ultimately result in emaciation or death caused by secondary bacterial infections. The infestation is usually transmitted by close contact between animals. Psoroptic mange is widespread in the world. In this paper, the transcriptome of P. ovis is described following sequencing and analysis of transcripts from samples of larvae (i.e. the Pso_L group) and nymphs and adults (i.e. the Pso_N_A group). The study describes differentially expressed genes (DEGs) and genes encoding allergens, which help understanding the biology of P. ovis and lay foundations for the development of vaccine antigens and drug target screening.MethodsThe transcriptome of P. ovis was assembled and analyzed using bioinformatic tools. The unigenes of P. ovis from each developmental stage and the unigenes differentially between developmental stages were compared with allergen protein sequences contained in the allergen database website to predict potential allergens.ResultsWe identified 38,836 unigenes, whose mean length was 825 bp. On the basis of sequence similarity with seven databases, a total of 17,366 unigenes were annotated. A total of 1,316 DEGs were identified, including 496 upregulated and 820 downregulated in the Pso_L group compared with the Pso_N_A group. We predicted 205 allergens genes in the two developmental stages similar to genes from other mites and ticks, of these, 14 were among the upregulated DEGs and 26 among the downregulated DEGs.ConclusionThis study provides a reference transcriptome of P. ovis in absence of a reference genome. The analysis of DEGs and putative allergen genes may lay the foundation for studies of functional genomics, immunity and gene expression profiles of this parasitic mite species.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1856-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

“…cDNA libraries of P. ovis were constructed by Kenyon et al [45] and Burgess et al [20], and a cDNA library of P. ovis var. cuniculi was constructed by Hu et al[46] .…”

Section: Discussionmentioning

confidence: 99%

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Preliminary analysis of Psoroptes ovis transcriptome in different developmental stages

et al. 2016

Parasites Vectors

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“…RNA was eluted in 14 ml molecular grade water (Sigma Aldrich, UK), and RNA quality assessed using Agilent Bioanalyser Pico Chips (Agilent, UK). RNA integrity numbers (RINs) were disregarded in favour of visual appraisal of the 18S ribosomal RNA (rRNA) electropherogram peak on the basis that the 21S rRNA fragment is either degraded or co-migrates with 18S, as observed for P. ovis and other arthropods [32,33].…”

Section: Rna Extractionmentioning

confidence: 99%

Gene silencing by RNA interference in the house dust mite, Dermatophagoides pteronyssinus

Marr

Sargison

Nisbet

et al. 2015

Molecular and Cellular Probes

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“…However, identification of the individual proteins involved in the development of this partially protective immunity has not yet been achieved. To further understand the mite:host interaction and identify suitable avenues for the discovery of novel interventions we have previously generated a P. ovis cDNA library and undertaken an expressed sequence tag (EST) approach [ 17 ]. This paper describes the use of these ESTs in the construction of a P. ovis cDNA microarray and the utilisation of this array, the first resource of its type for this economically important ectoparasite, under biological conditions to analyse mite gene expression during exposure to, and removal from, the host.…”

Section: Introductionmentioning

confidence: 99%

Development of a cDNA microarray for the measurement of gene expression in the sheep scab mite Psoroptes ovis

et al. 2012

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BackgroundSheep scab is caused by the ectoparasitic mite Psoroptes ovis which initiates a profound cutaneous inflammatory response, leading to the development of the skin lesions which are characteristic of the disease. Existing control strategies rely upon injectable endectocides and acaricidal dips but concerns over residues, eco-toxicity and the development of acaricide resistance limit the sustainability of this approach. In order to identify alternative means of disease control, a deeper understanding of both the parasite and its interaction with the host are required.MethodsHerein we describe the development and utilisation of an annotated P. ovis cDNA microarray containing 3,456 elements for the measurement of gene expression in this economically important ectoparasite. The array consists of 981 P. ovis EST sequences printed in triplicate along with 513 control elements. Array performance was validated through the analysis of gene expression differences between fed and starved P. ovis mites.ResultsSequences represented on the array include homologues of major house dust mite allergens and tick salivary proteins, along with factors potentially involved in mite reproduction and xenobiotic metabolism. In order to validate the performance of this unique resource under biological conditions we used the array to analyse gene expression differences between fed and starved P. ovis mites. These analyses identified a number of house dust mite allergen homologues up-regulated in fed mites and P. ovis transcripts involved in stress responses, autophagy and chemosensory perception up-regulated in starved mites.ConclusionThe P. ovis cDNA microarray described here has been shown to be both robust and reproducible and will enable future studies to analyse gene expression in this important ectoparasite.

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Generation, analysis and functional annotation of expressed sequence tags from the ectoparasitic mite Psoroptes ovis

Cited by 12 publications

References 57 publications

Preliminary analysis of Psoroptes ovis transcriptome in different developmental stages

Preliminary analysis of Psoroptes ovis transcriptome in different developmental stages

Gene silencing by RNA interference in the house dust mite, Dermatophagoides pteronyssinus

Development of a cDNA microarray for the measurement of gene expression in the sheep scab mite Psoroptes ovis

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