Generating vascular channels within hydrogel constructs using an economical open-source 3D bioprinter and thermoreversible gels

Fitzsimmons, Ross; Aquilino, Mark S.; Quigley, Jasmine; Chebotarev, Oleg; Tarlan, Farhang; Simmons, Craig A.

doi:10.1016/j.bprint.2018.02.001

Cited by 43 publications

(33 citation statements)

References 20 publications

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“…For example, the RepRap project 3D printers borrowed heavily from standard software and hardware Computer Numeric Control (CNC) tools used in machining. As open source designs, electronics boards, software, and parts for 3D printers were continually published and improved, cheap and interchangeable open source hardware and software intended for 3D printing began to be repurposed for new bioinstruments such as liquid handlers 14 , vial handlers and food dispensers 15 , autosamplers 16,17 , and bioprinters 18,19 .…”

Section: Introductionmentioning

confidence: 99%

Principles of open source bioinstrumentation applied to the poseidon syringe pump system

Booeshaghi

Beltrame

Bannon

et al. 2019

Sci Rep

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The poseidon syringe pump and microscope system is an open source alternative to commercial systems. It costs less than $400 and can be assembled in under an hour using the instructions and source files available at https://pachterlab.github.io/poseidon . We describe the poseidon system and use it to illustrate design principles that can facilitate the adoption and development of open source bioinstruments. The principles are functionality, robustness, safety, simplicity, modularity, benchmarking, and documentation.

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Section: Introductionmentioning

confidence: 99%

Principles of open source bioinstrumentation applied to the poseidon syringe pump system

Booeshaghi

Beltrame

Bannon

et al. 2019

Sci Rep

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“…The Inkredible + 3D-bioprinter (Cellink, Sweden) was used in our studies. Pluronic F127(Cellink, Sweden) was used as the printing material to create the molds used for casting the PDMS structures 50 . Pluronic is a printable ink with a wide range of applications such as low molecular weight component fabrication to sacrificial material or support structures, useful to create microchannels and vascularized tissues 51 .…”

Section: Methodsmentioning

confidence: 99%

3D-bioprinted all-inclusive bioanalytical platforms for cell studies

Mazrouei

Velasco

Esfandyarpour

2020

Sci Rep

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Innovative drug screening platforms should improve the discovery of novel and personalized cancer treatment. Common models such as animals and 2D cell cultures lack the proper recapitulation of organ structure and environment. Thus, a new generation of platforms must consist of cell models that accurately mimic the cells' microenvironment, along with flexibly prototyped cell handling structures that represent the human environment. Here, we adapted the 3D-bioprinting technology to develop multiple all-inclusive high throughputs and customized organ-on-a-chip-like platforms along with printed 3D-cell structures. Such platforms are potentially capable of performing 3D cell model analysis and cell-therapeutic response studies. We illustrated spherical and rectangular geometries of bio-printed 3D human colon cancer cell constructs. We also demonstrated the utility of directly 3D-bioprinting and rapidly prototyping of PDMS-based microfluidic cell handling arrays in different geometries. Besides, we successfully monitored the post-viability of the 3D-cell constructs for seven days. Furthermore, to mimic the human environment more closely, we integrated a 3D-bioprinted perfused drug screening microfluidics platform. Platform's channels subject cell constructs to physiological fluid flow, while its concave well array hold and perfused 3D-cell constructs. The bioapplicability of PDMS-based arrays was also demonstrated by performing cancer cell-therapeutic response studies. Traditionally, biomedical research has relied on animal models or two-dimensional (2D) cell cultures. Animal models, though one of the most commonly used systematic models, provide a limited understanding of humanspecific biology of different tissues. This is due to several reasons, such as fundamental differences between humans and animals 1 , low throughput studies, in addition to the ethical concerns 2. Animal testing is not costeffective, considering the cost to provide care, food, and shelter for the animals. Moreover, it is still possible that animal models can show promising results for drug treatments that can be harmful when tested on human subjects 3. On the other hand, despite the broad applications of 2D-cell cultures, these models only interact with their microenvironment in two dimensions, which in most cases cannot properly represent physiological conditions 4. Several studies have shown that 2D-cell constructs possessed altered cell polarity, mechanical cues, biochemical signals, and cell-cell interactions 5. Recently, there has been an emergence of three-dimensional (3D) cell models that better capture the complex cellular microenvironment than the conventional 2D models. 3D-models have shown improvement and relevance in vivo cell structure and function, where features such as the cell type, cell morphology, cell propagation, as well as, differentiation are more precisely represented 6-13. Furthermore, one should note that each year, billions of dollars are wasted because of preclinical 2D cell culture failure in predicting drug safety ...

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“…3D printers are of interest as they are addressable by scripting, cheap and have a supporting user community. Fitzsimmons et al 18 recently generated a low-cost bio printer that is conceived to be modular and open source 18 . Users have the convenience to assemble the printers and accomplish iterative enhancements on their own.…”

Section: Introductionmentioning

confidence: 99%

“…npj Biofilms and Microbiomes (2020)18 Published in partnership with Nanyang Technological University 1234567890():,;…”

mentioning

confidence: 99%

An open-source robotic platform that enables automated monitoring of replicate biofilm cultivations using optical coherence tomography

Gierl

Støy

Faina

et al. 2020

npj Biofilms Microbiomes

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The paper introduces a fully automated cultivation and monitoring tool to study biofilm development in replicate experiments operated in parallel. To gain a fundamental understanding of the relation between cultivation conditions and biofilm characteristics (e.g., structural, mechanical) a monitoring setup allowing for the standardization of methods is required. Optical coherence tomography (OCT) is an imaging modality ideal for biofilms since it allows for the monitoring of structure in real time. By integrating an OCT device into the open-source robotic platform EvoBot, a fully automated monitoring platform for investigating biofilm development in several flow cells at once was realized. Different positioning scenarios were tested and revealed that the positioning accuracy is within the optical resolution of the OCT. On that account, a reliable and accurate monitoring of biofilm development by means of OCT has become possible. With this robotic platform, reproducible biofilm experiments including a statistical analysis are achievable with only a small investment of operator time. Furthermore, a number of structural parameters calculated within this study confirmed the necessity to perform replicate biofilm cultivations.npj Biofilms and Microbiomes (2020) 6:18 ; https://doi.

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Generating vascular channels within hydrogel constructs using an economical open-source 3D bioprinter and thermoreversible gels

Cited by 43 publications

References 20 publications

Principles of open source bioinstrumentation applied to the poseidon syringe pump system

Principles of open source bioinstrumentation applied to the poseidon syringe pump system

3D-bioprinted all-inclusive bioanalytical platforms for cell studies

An open-source robotic platform that enables automated monitoring of replicate biofilm cultivations using optical coherence tomography

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