Generating Potent and Selective Inhibitors of Kv1.3 Ion Channel by Fusing Knottins (Venom Derived Mini Proteins) into Peripheral CDR Loops of Antibodies

Abstract

surface to a large cytoplasmic cavity, and a unique configuration of the photoactive site likely responsible for the delayed Schiff base deprotonation. Using this structural information, we have identified residues that determine absorption wavelength and current-voltage dependence. Our results provide clues for rational engineering of ACR molecules further to increase their optogenetic utility.