Generating Ceramide from Sphingomyelin by Alkaline Sphingomyelinase in the Gut Enhances Sphingomyelin-Induced Inhibition of Cholesterol Uptake in Caco-2 Cells

Feng, Dan; Ohlsson, Lena; Ling, Wenhua; Nilsson, Åke; Duan, Rui‐Dong

doi:10.1007/s10620-010-1202-9

Cited by 28 publications

(23 citation statements)

References 38 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has also been suggested that ceramide, the hydrolytic product of SM, inhibits intestinal cholesterol uptake [4]. Since the degradation of ceramide along the gastrointestinal tract is relatively slow and inefficient, it is possible that the inhibitory effect of ceramide on cholesterol absorption could be quite significant [19].…”

Section: Discussionmentioning

confidence: 99%

“…SM is a sphingolipid found in foods such as milk and eggs. Purified SM has been shown to inhibit cholesterol absorption in a number of acute in vitro [4] and in vivo [5]–[8] studies. A recent study reported plasma and hepatic lipid lowering effects in Zucker fatty rats fed a low-fat chow diet supplemented with chicken skin-derived SM for 6 weeks [9].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dietary Sphingomyelin Lowers Hepatic Lipid Levels and Inhibits Intestinal Cholesterol Absorption in High-Fat-Fed Mice

et al. 2013

View full text Add to dashboard Cite

Controlling intestinal lipid absorption is an important strategy for maintaining lipid homeostasis. Accumulation of lipids in the liver is a major risk factor for metabolic syndrome and nonalcoholic fatty liver disease. It is well-known that sphingomyelin (SM) can inhibit intestinal cholesterol absorption. It is, however, unclear if dietary SM also lowers liver lipid levels. In the present study (i) the effect of pure dietary egg SM on hepatic lipid metabolism and intestinal cholesterol absorption was measured with [14C]cholesterol and [3H]sitostanol in male C57BL/6 mice fed a high-fat (HF) diet with or without 0.6% wt/wt SM for 18 days; and (ii) hepatic lipid levels and gene expression were determined in mice given a HF diet with or without egg SM (0.3, 0.6 or 1.2% wt/wt) for 4 weeks. Mice supplemented with SM (0.6% wt/wt) had significantly increased fecal lipid and cholesterol output and reduced hepatic [14C]cholesterol levels after 18 days. Relative to HF-fed mice, SM-supplemented HF-fed mice had significantly lower intestinal cholesterol absorption (−30%). Liver weight was significantly lower in the 1.2% wt/wt SM-supplemented mice (−18%). Total liver lipid (mg/organ) was significantly reduced in the SM-supplemented mice (−33% and −40% in 0.6% wt/wt and 1.2% wt/wt SM, respectively), as were triglyceride and cholesterol levels. The reduction in liver triglycerides was due to inactivation of the LXR-SREBP-1c pathway. In conclusion, dietary egg SM has pronounced hepatic lipid-lowering properties in mice maintained on an obesogenic diet.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dietary Sphingomyelin Lowers Hepatic Lipid Levels and Inhibits Intestinal Cholesterol Absorption in High-Fat-Fed Mice

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Noh and Koo compared the inhibitory effects of SM from different sources, milk and egg, on cholesterol absorption, and found that milk SM had a stronger inhibitory effect probably due to higher saturation degree and longer chain of fatty acyl group in SM [98]. Feng found that ceramide, the hydrolytic product of SM, had a stronger inhibitory effect on cholesterol uptake in intestinal cells [191]. The possible mechanisms of inhibitory effect of phospholipids are discussed in Section 5.2.…”

Section: The Relationship Between Egg Intake and Cvd Risksmentioning

confidence: 99%

The Impact of Egg Nutrient Composition and Its Consumption on Cholesterol Homeostasis

et al. 2018

View full text Add to dashboard Cite

Nutrient deficiencies and excess are involved in many aspects of human health. As a source of essential nutrients, eggs have been used worldwide to support the nutritional needs of human societies. On the other hand, eggs also contain a significant amount of cholesterol, a lipid molecule that has been associated with the development of cardiovascular diseases. Whether the increase of egg consumption will lead to elevated cholesterol absorption and disruption of cholesterol homeostasis has been a concern of debate for a while. Cholesterol homeostasis is regulated through its dietary intake, endogenous biosynthesis, utilization, and excretion. Recently, some research interests have been paid to the effects of egg consumption on cholesterol homeostasis through the intestinal cholesterol absorption. Nutrient components in eggs such as phospholipids may contribute to this process. The goals of this review are to summarize the recent progress in this area and to discuss some potential benefits of egg consumption.

show abstract

“…This aforementioned inhibitory effect is a result of direct interaction between SM and cholesterol leading to a decreased cholesterol thermodynamic activity [53]. Moreover, Feng et al [54] showed that cholesterol absorption is also inhibited by ceramide formed from SM through the activation of alkaline sphingomyelinase [54]. Those authors proved that ceramide, as an inhibitor of cholesterol absorption, is more effective than SM [54].…”

Section: Physiological Role Of Sphingolipids In Gastrointestinal Tmentioning

confidence: 99%

“…Moreover, Feng et al [54] showed that cholesterol absorption is also inhibited by ceramide formed from SM through the activation of alkaline sphingomyelinase [54]. Those authors proved that ceramide, as an inhibitor of cholesterol absorption, is more effective than SM [54]. Moreover, cholesterol uptake by intestinal cells is suppressed by sphingosine, but it is less effective than SM and ceramide [55].…”

Section: Physiological Role Of Sphingolipids In Gastrointestinal Tmentioning

confidence: 99%

Metabolism, Physiological Role, and Clinical Implications of Sphingolipids in Gastrointestinal Tract

Kurek

Łukaszuk

Piotrowska

et al. 2013

BioMed Research International

View full text Add to dashboard Cite

Sphingolipids in digestive system are responsible for numerous important physiological and pathological processes. In the membrane of gut epithelial cells, sphingolipids provide structural integrity, regulate absorption of some nutrients, and act as receptors for many microbial antigens and their toxins. Moreover, bioactive sphingolipids such as ceramide or sphingosine-1-phosphate regulate cellular growth, differentiation, and programmed cell death—apoptosis. Although it is well established that sphingolipids have clinical implications in gastrointestinal tumorigenesis or inflammation, further studies are needed to fully explore the role of sphingolipids in neoplastic and inflammatory diseases in gastrointestinal tract. Pharmacological agents which regulate metabolism of sphingolipids can be potentially used in the management of colorectal cancer or inflammatory bowel diseases. The aim of this work is to critically the review physiological and pathological roles of sphingolipids in the gastrointestinal tract.

show abstract

Generating Ceramide from Sphingomyelin by Alkaline Sphingomyelinase in the Gut Enhances Sphingomyelin-Induced Inhibition of Cholesterol Uptake in Caco-2 Cells

Abstract: Background Sphingomyelin (SM) is present in dietary products and cell plasma

Cited by 28 publications

References 38 publications

Dietary Sphingomyelin Lowers Hepatic Lipid Levels and Inhibits Intestinal Cholesterol Absorption in High-Fat-Fed Mice

Dietary Sphingomyelin Lowers Hepatic Lipid Levels and Inhibits Intestinal Cholesterol Absorption in High-Fat-Fed Mice

The Impact of Egg Nutrient Composition and Its Consumption on Cholesterol Homeostasis

Metabolism, Physiological Role, and Clinical Implications of Sphingolipids in Gastrointestinal Tract

Contact Info

Product

Resources

About