Generalized transduction in the enterobacterial phytopathogen Erwinia chrysanthemi

Chatterjee, Asita; Brown, M A

doi:10.1128/jb.143.3.1444-1449.1980

Cited by 24 publications

(8 citation statements)

References 18 publications

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“…and used to study different aspects of the hosts, viz. bacterial cell wall structure (Expert & Toussaint, 1985), generalized transduction (Chatterjee & Brown, 1980), phage typing (Gross et al, 1991) and to estimate bacterial populations in complex environments (Suzuki & Togashi, 1978).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of novel soilborne lytic bacteriophages infecting Dickeya spp. biovar 3 (‘D. solani’)

2013

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Nine bacteriophages infecting Dickeya spp. biovar 3 ('Dickeya solani') were isolated from soil samples collected in different regions in Poland. The phages have a typical morphology of the members of the order Caudovirales, family Myoviridae, with a head diameter of c. 90-100 nm and tail length of c. 120-140 nm. In host range experiments, phage /D5 expressed the broadest host range, infecting members of all Dickeya spp., and phage /D7 showed the narrowest host range, infecting isolates of Dickeya dadantii and 'D. solani' only. None of the phages was able to infect Pectobacterium spp. isolates. All phages were prone to inactivation by pH 2, temperature of 85°C and by UV illumination for 10 min (50 mJ cm À2 ). Additionally, phages /D1, /D10 and /D11 were inactivated by 5 M NaCl and phage /D2 was inactivated by chloroform. Phages /D1, /D5, /D7 and /D10 were characterized for optimal multiplicity of infection and the rate of adsorption to the bacterial cells. The latent period was 30 min for /D1, 40 min for /D5, 20-30 min for /D7 and 40 min for /D10. The estimated burst size was c. 100 plaque-forming units per infected cell. The bacteriophages were able to completely stop the growth of 'D. solani' in vitro and to protect potato tuber tissue from maceration caused by the bacteria. The potential use of bacteriophages for the biocontrol of biovar 3 Dickeya spp. in potato is discussed.

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Section: Introductionmentioning

confidence: 99%

Isolation and characterization of novel soilborne lytic bacteriophages infecting Dickeya spp. biovar 3 (‘D. solani’)

2013

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“…However, various authors have used genetic approaches to elucidate pathogenic mechanisms in this bacterium and numerous genetic tools have been developed. There have been reports on genetic transfer by transformation (Reverchon and Robert-Baudouy, 1985;Hinton ef ai., 1985) and generalized transduction (Chatterjee and Brown, 1980;Resiboisefa/., 1984) as well as conjugation; various authors showed that the E. ciirysanthemi cells do not appear to exchange chromosomal DNA on their own but will act as conjugal donors if an F-factor or R-factor plasmid is present (Chatterjee and Starr, 1977;Chatterjee, 1980;Kotoujansky etai., 1982;Schoonejans and Toussaint, 1983;Chatterjee ef ai., 1985). Despite all these studies, no precise linkage map of a soft-rotting Erwinia has yet been described (about ten markers have been analysed on each strain).…”

Section: Introductionmentioning

confidence: 99%

Expanded linkage map of Erwinia chrysanthemi strain 3937

Hugouvieux‐Cotte‐Pattat

Reverchon

Robert‐Baudouy

1989

Molecular Microbiology

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In this paper we describe the chromosomal location of various loci in Erwinia chrysanthemi strain 3937. Auxotrophic markers were obtained by chemical mutagenesis, antibiotic resistances were isolated spontaneously and mutations in sugar utilization were obtained by means of Mu insertions. These markers were located on the genetic linkage map of strain 3937 by using a conjugative system mediated by RP4::mini-Mu plasmids which permitted transfer of genetic material from any point of origin. The location of these markers was compared to that of previously located mutations. Many genes involved in pectinolysis were also located on the E. chrysanthemi 3937 map. These results permitted us to present a new genetic map containing 61 markers distributed over 34 widely scattered loci on the chromosome. Some pairs of markers giving high cotransfer frequencies were tested for cotransduction mediated by the generalized transducing phage phi-EC2; nine cotransducing pairs were found. It appears that the chromosomal locations of many of these loci are quite different to those of the well-known enterobacterium Escherichia coli but seem similar to those described for other E. chrysanthemi strains.

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“…AC6070 is a spontaneous streptomycin-resistant derivative of AC6022 (his-3 leu-2 arg-i ilv-1) which was obtained by ethyl methanesulfonate mutagenesis of AC6017 (arg-I leu-2 ilv-l) (4).…”

Section: Carotovora Ecc193 Ac401mentioning

confidence: 99%

pULB113, an RP4::mini-Mu plasmid, mediates chromosomal mobilization and R-prime formation in Erwinia amylovora, Erwinia chrysanthemi, and subspecies of Erwinia carotovora

Chatterjee¹,

Ross²,

McEvoy³

et al. 1985

Appl Environ Microbiol

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The RP4::mini-Mu plasmid pULB113, transferred from Escherichia coli strain MXR, was stable and transfer proficient in Erwinia amylovora strain EA303, E. carotovora subsp. atroseptica strain ECA12, E. carotovora subsp. carotovora strain ECC193, and E. chrysanthemi strain EC183. The plasmid mobilized an array of Erwinia sp. chromosomal markers (E. amylovora: his+, ilv+, rbs+, ser+, thr+; E. chrysanthemi: arg+, his+, ilv+, leu+; E. carotovora subsp. atroseptica: arg+, gua+, leu+, lys+, pur+, trp+; E. carotovora subsp. carotovora: arg+, gua+, leu+, lys+, out+ [export of enzymes], pur+, trp+), suggesting random interactions of the plasmid with the chromosomes. In E. carotovora subsp. carotovora, pULB113-mediated two-factor crosses revealed linkage between three auxotrophic markers and the out loci. The export of pectate lyase, polygalacturonase, and cellulase and the maceration of potato tuber tissue occurred with Out+, but not Out-, strains of E. carotovora subsp. carotovora, indicating the importance of enzyme export in plant tissue maceration. Erwinia sp. donors harboring pULB113 complemented mutations in various biosynthetic and catabolic genes (arg, gal, his, leu, met, pro, pur, thy) in Escherichia coli recA strains. Escherichia coli transconjugants harbored pULB113 primes as indicated by the cotransfer of Erwinia genes and pULB113 markers and a change in plasmid mass. Moreover, the Pstl and SmaI cleavage patterns of selected pULB113 primes were different from those of pULB113. pULB113 primes carried DNA insertions ranging from 3 to about 160 kilobases. These findings indicate that pULB113 is useful for in vivo gene cloning and genetic analysis of various enterobacterial phytopathogens.

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Generalized transduction in the enterobacterial phytopathogen Erwinia chrysanthemi

Cited by 24 publications

References 18 publications

Isolation and characterization of novel soilborne lytic bacteriophages infecting Dickeya spp. biovar 3 (‘D. solani’)

Isolation and characterization of novel soilborne lytic bacteriophages infecting Dickeya spp. biovar 3 (‘D. solani’)

Expanded linkage map of Erwinia chrysanthemi strain 3937

pULB113, an RP4::mini-Mu plasmid, mediates chromosomal mobilization and R-prime formation in Erwinia amylovora, Erwinia chrysanthemi, and subspecies of Erwinia carotovora

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