In this manuscript, a generalized inverse eigenvalue problem is considered that involves a linear pencil (zJ [0,n] − H [0,n] ) of (n + 1) by (n + 1) matrices arising in the theory of rational interpolation and biorthogonal rational functions. In addition to the reconstruction of the Hermitian matrix H [0,n] , characterizations of the rational functions that are components of the prescribed eigenvectors are given. A condition concerning the positive-definiteness of J [0,n] which is often an assumption in the direct problem is also isolated. Further, the reconstruction of H [0,n] is viewed through the inverse of the pencil (zJ [0,n] − H [0,n] ) which involves the concept of m-functions.