Generalized Implementation of Rapid-Scan Fourier Transform Infrared Spectroscopic Imaging

Huffman, Scott W.; Bhargava, Rohit; Levin, Ira W.

doi:10.1366/000370202760249684

Cited by 35 publications

(21 citation statements)

References 13 publications

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“…The state of the art in this field is then represented by a system composed of a microscope with an FPA and synchrotron radiation. This setup already enhances all FTIR capabilities but in the future we will certainly see new integrated systems designed to match the projected size of the synchrotron radiation source in order to preserve the SR brilliance and allowing faster data acquisition [47,48].…”

Section: Discussionmentioning

confidence: 99%

Protein Structure and Function in the Time-Domain of Vibrational Spectroscopies. The Promising Applications of IR Synchrotron Radiation Micro-Spectroscopy

Marcelli

2006

Acta Phys. Pol. A

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Fourier Transform Infrared (FTIR) spectroscopy is a fundamental technique capable to characterize proteins and to investigate their conformation and dynamics in real physiological environments. Actually, a FTIR spectrum is characterized by many features, which may be correlated to the different components of the protein structure. In the last decade many relevant results have been achieved with this technique in terms of chemical imaging of proteins at subcellular level and in the investigation of cooperative phenomena. This contribution presents a few examples that illustrate the capability of the FTIR spectroscopy to investigate both protein structure and function and the opportunities offered by IR synchrotron radiation sources. Indeed the high source brilliance of these sources enables FTIR micro-spectroscopy to be performed with spatial and time resolution not available with standard sources. Moreover, the combination of synchrotron radiation and new two-dimensional detectors open new opportunities to investigate in the IR energy domain different protein processes in real time and with proteins in their native environments.

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Section: Discussionmentioning

confidence: 99%

Protein Structure and Function in the Time-Domain of Vibrational Spectroscopies. The Promising Applications of IR Synchrotron Radiation Micro-Spectroscopy

Marcelli

2006

Acta Phys. Pol. A

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“…At the end of the 20th century, IMS instruments based on standard thermal IR sources were equipped with focal plane array (FPA) detectors (13,14). FPA detectors opened a new generation of IMS (15).…”

Section: Stage Ii: Coexistence Of Thermal and Synchrotron Infrared Somentioning

confidence: 99%

The Development of Infrared Microspectroscopy (IMS) and Its Applications in Agricultural and Aquatic Products

Zhou

Fang

2013

Applied Spectroscopy Reviews

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Infrared microspectroscopy (IMS) has undergone rapid development in the last 20 years. New infrared sources and detectors have promoted this development. More meaningful information can be achieved in less time, and spatial resolution is only several micrometers. The improved IMS is important in biology, medical science, and biochemistry. This review describes the progress of IMS in light sources and detectors, including thermal sources, synchrotron infrared sources, multiple synchrotron beams, single element detectors, as well as focal plane array (FPA) detectors. In addition, applications of IMS on agricultural and aquatic products such as cereal seeds, milk, fish, meat, forage grass, juice, and cheese are explored. Finally, new trends in the development of IMS and its importance in the fundamental and applied research of agricultural and aquatic products are discussed.

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“…Although the Javelin camera as installed in the missile has a frame rate of 180 Hz, SBFP, using their own warm electronics, successfully ran it with a frame rate as high as 419 Hz, with 316 Hz being a typical operating rate. Thus, it was necessary for commercial instruments using the Javelin detector to use step-scan interferometers, although some rapid-scan experiments using high undersampling rates were performed [65,66]. The development of this ''Javelin camera'' based imaging system initiated a second wave of imaging applications development [67][68][69][70], including the first imaging kinetics studies by Koenig [71][72][73].…”

Section: The ''Javelin'' Mct Cameramentioning

confidence: 99%

FT‐IR Imaging Hardware

Sellors

Hoult

Crocombe

et al. 2010

Raman, Infrared, and Near‐Infrared Chemical Imaging

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The combination of IR spectroscopy with visible microscopy has been used in a wide range of analytical applications for more than 20 years. More recently, however, IR microspectroscopy has benefited from developments in IR detector arrays leading to a marked growth in FT-IR imaging technologies and applications. It is now a fairly simple task to obtain a high-quality IR spectrum from a sample region of around 20 mm in matter of seconds, and the ability to collect full IR images containing hundreds of thousands of pixels, where every image pixel contains a full range IR spectrum, is now available in many hundreds of laboratories worldwide. IR imaging hardware is not yet mature, but despite this, with today's state-of-the-art FT-IR imaging systems, the analysis time for many applications is limited not by the speed at which quality images are obtained, but by the data analysis or sample preparation techniques at the disposal of the operator.Progress in commercial FT-IR imaging hardware development comes from various drivers, but two are particularly relevant: (a) the high popularity of single point IR microscopy systems that has fuelled the interest in technology and applications utilizing more rapid methods of data acquisition and (b) the development of multichannel array detectors that operate in the mid-infrared region for nonspectroscopic applications. These are fundamental to both the understanding of current FT-IR imaging technologies and probable developments in the near future. Developments in IR Microscopy and Imaging SystemsInterest in obtaining IR spectra from small samples goes back to over 60 years; for example, a reported study of the structure of penicillin by Thomson [1] in the late 1940s used a prismbased dispersive spectrometer coupled with a beam condenser/microscope system. A commercial IR microscope system described in 1953 by Coates et al. [2] demonstrated quite respectable IR spectra from single fiber samples of less than 20 mm in diameter, recorded with 15 min scan times, and contained some design attributes that are still present in today's systems. However, it was not until early 1980s when the rapid uptake of commercial FT-IR systems and applications such as semiconductor microanalysis provided both the applications and technology interest to spur the growth in IR microspectroscopy. Today, all major manufacturers of laboratory FT-IR spectrometers include IR microscopes, some with automated point mapping systems, as well as imaging systems in their product portfolios.Early (1980s) FT-IR microscope systems were mostly bolt-on accessories derived from optical microscope frames that were modified to support the collection of IR spectra from selected regions of interest (ROI). Later, systems were Raman, Infrared, and Near-Infrared Chemical Imaging Edited by Slobodan Š ašić and Yukihiro Ozaki

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Generalized Implementation of Rapid-Scan Fourier Transform Infrared Spectroscopic Imaging

Cited by 35 publications

References 13 publications

Protein Structure and Function in the Time-Domain of Vibrational Spectroscopies. The Promising Applications of IR Synchrotron Radiation Micro-Spectroscopy

Protein Structure and Function in the Time-Domain of Vibrational Spectroscopies. The Promising Applications of IR Synchrotron Radiation Micro-Spectroscopy

The Development of Infrared Microspectroscopy (IMS) and Its Applications in Agricultural and Aquatic Products

FT‐IR Imaging Hardware

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