Generalization of Courtship Learning in Drosophila Is Mediated by cis-Vaccenyl Acetate

Ejima, Aki; Smith, B. L.; Lucas, Christophe; Naters, Wynand van der Goes van; Miller, Carson J.; Carlson, John R.; Levine, Joel D.; Griffith, Leslie C.

doi:10.1016/j.cub.2007.01.053

Cited by 249 publications

(307 citation statements)

References 39 publications

(44 reference statements)

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“…In addition, the expression pattern of Eph-myc in the pupal optic lobe was consistent with the previous report (Supplemental Fig. S1D Ejima et al 2007;Kurtovic et al 2007;van der Goes van Naters and Carlson 2007;Grosjean et al 2011;Liu et al 2011;Lebreton et al 2014;Lin et al 2016). Interestingly, the expression was markedly low in the neighboring regions of the Eph-positive glomeruli (Supplemental Fig.…”

Section: Loss Of Eph or Ephrin Exhibits A Dendritic Spillover Phenotysupporting

confidence: 91%

“…Among 50 glomeruli, the DA1, DL3, VA1lm, and VL2a glomeruli exhibited high Eph expression during development, holding the line against the other glomeruli by their unique function. Unlike other glomeruli, the DA1, DL3, VA1lm, and VL2a glomeruli receive inputs from the axons of ORNs (Or67d, Or65a, Or47b, and Ir84a, respectively) dedicated to sensing odors related to reproductive behavior, such as pheromones and food-derived odors promoting male courtship behaviors (Ejima et al 2007;Kurtovic et al 2007;van der Goes van Naters and Carlson 2007;Grosjean et al 2011;Liu et al 2011;Lebreton et al 2014;Lin et al 2016). Among them, Or67d, the primary neuron of the DA1 olfactory circuit, detects Drosophila male-specific pheromone 11 cis-vaccenyl acetate (cVA) and triggers sex-specific courtship behavior in both male and female flies (Kurtovic et al 2007).…”

Section: Glomerular Boundary Formation By Eph/ephrin For the Pheromonmentioning

confidence: 99%

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Dendritic Eph organizes dendrodendritic segregation in discrete olfactory map formation in Drosophila

Anzo¹,

Sekine²,

Makihara³

et al. 2017

Genes Dev.

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Proper function of the neural network results from the precise connections between axons and dendrites of presynaptic and postsynaptic neurons, respectively. In the Drosophila olfactory system, the dendrites of projection neurons (PNs) stereotypically target one of ∼50 glomeruli in the antennal lobe (AL), the primary olfactory center in the brain, and form synapses with the axons of olfactory receptor neurons (ORNs). Here, we show that Eph and Ephrin, the well-known axon guidance molecules, instruct the dendrodendritic segregation during the discrete olfactory map formation. The Eph receptor tyrosine kinase is highly expressed and localized in the glomeruli related to reproductive behavior in the developing AL. In one of the pheromone-sensing glomeruli (DA1), the Eph cell-autonomously regulates its dendrites to reside in a single glomerulus by interacting with Ephrins expressed in adjacent PN dendrites. Our data demonstrate that the trans interaction between dendritic Eph and Ephrin is essential for the PN dendritic boundary formation in the DA1 olfactory circuit, potentially enabling strict segregation of odor detection between pheromones and the other odors.

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Section: Loss Of Eph or Ephrin Exhibits A Dendritic Spillover Phenotysupporting

confidence: 91%

Section: Glomerular Boundary Formation By Eph/ephrin For the Pheromonmentioning

confidence: 99%

Dendritic Eph organizes dendrodendritic segregation in discrete olfactory map formation in Drosophila

Anzo¹,

Sekine²,

Makihara³

et al. 2017

Genes Dev.

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“…Hydrocarbon expression has been shown to change dramatically after courtship (6,10,12,15). To determine whether these changes could be detected by using DART MS in the same animal, individual female f lies were sampled before and at two time points after successful copulation.…”

Section: Profiling Of Male and Female Fliesmentioning

confidence: 99%

Cuticular hydrocarbon analysis of an awake behaving fly using direct analysis in real-time time-of-flight mass spectrometry

Yew

Cody

Kravitz

2008

Proc. Natl. Acad. Sci. U.S.A.

114

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In mammals and insects, pheromones strongly influence social behaviors such as aggression and mate recognition. In Drosophila melanogaster, pheromones in the form of cuticular hydrocarbons play prominent roles in courtship. GC/MS is the primary analytical tool currently used to study Drosophila cuticular hydrocarbons. Although GC/MS is highly reproducible and sensitive, it requires that the fly be placed in a lethal solution of organic solvent, thereby impeding further behavioral studies. We present a technique for the analysis of hydrocarbons and other surface molecules from live animals by using direct analysis in real-time (DART) MS. Cuticular hydrocarbons were sampled from the surface of a restrained, awake behaving fly by using several brief, carefully controlled depressions of the abdomen with a small steel probe. DART mass spectral analysis of the probe detected ions with mass-to-charge ratio (m/z) of the protonated molecule corresponding to many of the previously identified unsaturated hydrocarbons. Six additional cuticular hydrocarbons also were identified. Consistent with previous GC/MS studies, male and female differences in chemical composition were evident. Spatial differences in the expression profile also were observed on males. Sampling from an individual female first as a virgin and then 45 and 90 min after successful copulation showed that mass signals likely to correspond to cis-vaccenyl acetate, tricosene, and pentacosene increased in relative intensity after courtship. This method provides near-instantaneous analysis of an individual animal's chemical profile in parallel with behavioral studies and could be extended to other models of pheromone-mediated behavior.behavior ͉ cis-vaccenyl acetate ͉ courtship ͉ pheromones ͉ Drosophila

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“…These glomeruli are present in both male and female fly brains, but they occupy a significantly greater volume in the male AL (Stockinger et al 2005). DA1 is targeted by Or67d-expressing OSNs, which respond to the aversive pheromone 11-cis-vaccenyl-acetate (cVA) (Clyne et al 1997;Ejima et al 2007;Kurtovic et al 2007;van der Goes van Naters and Carlson 2007;Billeter et al 2009). Or67d-expressing OSNs are found in trichoid sensilla, which project to the region of the LH that is thought to be exclusively devoted to the processing of pheromone signals (Jefferis et al 2007).…”

mentioning

confidence: 99%

A Novel Gene Controlling the Timing of Courtship Initiation in Drosophila melanogaster

et al. 2016

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Over the past 35 years, developmental geneticists have made impressive progress toward an understanding of how genes specify morphology and function, particularly as they relate to the specification of each physical component of an organism. In the last 20 years, male courtship behavior in Drosophila melanogaster has emerged as a robust model system for the study of genetic specification of behavior. Courtship behavior is both complex and innate, and a single gene, fruitless (fru), is both necessary and sufficient for all aspects of the courtship ritual. Typically, loss of male-specific Fruitless protein function results in male flies that perform the courtship ritual incorrectly, slowly, or not at all. Here we describe a novel requirement for fru: we have identified a group of cells in which male Fru proteins are required to reduce the speed of courtship initiation. In addition, we have identified a gene, Trapped in endoderm 1 (Tre1), which is required in these cells for normal courtship and mating behavior. Tre1 encodes a G-protein-coupled receptor required for establishment of cell polarity and cell migration and has previously not been shown to be involved in courtship behavior. We describe the results of feminization of the Tre1-expressing neurons, as well as the effects on courtship behavior of mutation of Tre1. In addition, we show that Tre1 is expressed in a sexually dimorphic pattern in the central and peripheral nervous systems and investigate the role of the Tre1 cells in mate identification. KEYWORDS courtship; Fruitless; Drosophila; G-protein signaling; behavior O NE of the goals of neuroscience is to understand how genetic programs direct neurodevelopment and how the resulting neural pathways interact with sensory inputs to result in behavior. Drosophila melanogaster courtship behavior provides an excellent model system to study this process. Male Drosophila carry out a complex courtship ritual, the correct performance of which affects their success in mating and reproduction (Baker et al. 2001). This ritual consists of a series of stereotyped behaviors. The male first orients toward and follows the female, then taps her with his forelegs, extends one wing toward the female and vibrates it to generate a species-specific courtship song, licks her genitalia, and, finally, curls his abdomen to attempt copulation (Hall 1994). If the female is receptive (i.e., virgin or not recently mated) and the ritual is performed correctly, copulation will follow. These behavioral outputs all require sensory input and integration (Dickson 2008), and all are dependent upon expression of the male-specific isoforms of the zinc-finger transcription factor Fru (Fru M ) (Demir and Dickson 2005).The fru gene spans 130 kb of genomic DNA and generates at least 15 transcripts and six polypeptides (Demir and Dickson 2005). Most fru transcripts are common to males and females, but transcripts initiated from one of the four fru promoters (P1) are sex-specifically spliced under the control of the sexdetermining splicing ...

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Generalization of Courtship Learning in Drosophila Is Mediated by cis-Vaccenyl Acetate

Cited by 249 publications

References 39 publications

Dendritic Eph organizes dendrodendritic segregation in discrete olfactory map formation in Drosophila

Dendritic Eph organizes dendrodendritic segregation in discrete olfactory map formation in Drosophila

Cuticular hydrocarbon analysis of an awake behaving fly using direct analysis in real-time time-of-flight mass spectrometry

A Novel Gene Controlling the Timing of Courtship Initiation in Drosophila melanogaster

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