General stress, detoxification pathways, neurotoxicity and genotoxicity evaluated in Ruditapes philippinarum exposed to human pharmaceuticals

Aguirre-Martínez, G.V.; DelValls, T.À.; Martı́n-Dı́az, M.L.

doi:10.1016/j.ecoenv.2015.09.031

Cited by 116 publications

(58 citation statements)

References 111 publications

(140 reference statements)

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“…Among them, oxidative stress has been reported as an impact of CBZ following its accumulation and/or metabolization in this group of organisms (e.g. Aguirre-Martínez et al, 2016;Almeida et al, 2017aAlmeida et al, , 2015Almeida et al, , 2014Contardo-Jara et al, 2011;Freitas et al, 2016Freitas et al, , 2015bJuhel et al, 2017;Martin-Diaz et al, 2009;Tsiaka et al, 2013).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Effects of single and combined exposure of pharmaceutical drugs (carbamazepine and cetirizine) and a metal (cadmium) on the biochemical responses of R. philippinarum

et al. 2018

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In the aquatic environment, organisms are exposed to complex mixtures of contaminants which may alter the toxicity profile of each compound, compared to its toxicity alone. Pharmaceutical drugs (e.g. carbamazepine (CBZ) and cetirizine (CTZ)) and metals (e.g. cadmium (Cd)) are among those contaminants that co-occur in the environment. However, most studies concerning their toxicity towards aquatic species are based on single exposure experiments. Thus, the present study aimed to evaluate single and combined effects of Cd and CBZ or CTZ (single conditions: Cd, CTZ, CBZ; combined conditions: CTZ + Cd, CBZ + Cd) on biomarkers related to oxidative stress and energy metabolism in the edible clam Ruditapes philippinarum, by exposing the organisms for 28 days to environmentally relevant concentrations of these contaminants. The biomarkers studied were: i) the electron transport system activity, protein and glycogen contents (indicators of organisms' metabolic status and energy reserves); ii) lipid peroxidation and the ratio between reduced and oxidized glutathione (indicators of oxidative stress); iii) superoxide dismutase and catalase activities (enzymes indicators of antioxidant defence) and iv) activity of glutathione S-transferases (family of enzymes indicators of biotransformation capacity). Results obtained showed that the uptake of Cd and CBZ was not affected by the combined presence of the contaminants. However, for CTZ, the uptake was higher in the presence than in the absence of Cd. Concerning toxicity data, in general, the combined exposures (CTZ + Cd, CBZ + Cd) had lower biological effects than the contaminants alone. Nevertheless, our data showed that despite the low concentrations tested, they were enough to exert biological effects that differed between single and combined treatments, evidencing the need to conduct more co-exposure studies to increase the environmental relevance of the gathered data.

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Section: Accepted Manuscriptmentioning

confidence: 99%

Effects of single and combined exposure of pharmaceutical drugs (carbamazepine and cetirizine) and a metal (cadmium) on the biochemical responses of R. philippinarum

et al. 2018

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“…Although AChE activity was not assessed, very similar responses were obtained in the freshwater clam Corbicula fluminea exposed for 21 days to the same caffeine concentrations tested in R. philippinarum [54]. Impairment in lysosomal membrane stability of hemocytes after exposure to caffeine at doses as low as 0.05-0.1 µg/L suggest that immune cells can be a target for this substance in invertebrates [52][53][54][55], showing immune modulatory effects like those observed in rodents and humans [56].…”

Section: Discussionmentioning

confidence: 69%

“…Values of biomarkers of oxidative stress and biotrasformation phase I and II were significantly higher in digestive glands, mostly at the highest caffeine concentrations tested. Acetylcholinesterase (AChE) activity, as a biomarker of neurotoxicity, increased significantly at 0.1 µg/L, but was significantly inhibited at all the other concentrations [53]. Although AChE activity was not assessed, very similar responses were obtained in the freshwater clam Corbicula fluminea exposed for 21 days to the same caffeine concentrations tested in R. philippinarum [54].…”

Section: Discussionmentioning

confidence: 87%

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Exposure to Decreased pH and Caffeine Affects Hemocyte Parameters in the Mussel Mytilus galloprovincialis

Munari

Matozzo

Benetello

et al. 2020

JMSE

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Combined effects of reduced pH, as predicted under climate change scenarios, and the most popular and widely used stimulant caffeine were assessed in hemocyte parameters of the mussel Mytilus galloprovincialis, being hemocytes involved in immune defense. Bivalves were exposed for one week to natural pH (8.1) and two reduced pH values (pH −0.4 units and pH −0.7 units). Exposure continued for additional two weeks, both in the absence and in the presence of environmentally relevant concentrations of caffeine (0.05 and 0.5 µg/L). Hemocyte parameters (total hemocyte count, hemocyte volume and diameter, neutral red uptake and hemocyte proliferation) were measured after 7 days of exposure to pH only, and after 14 (T1) and 21 (T2) days of exposure to the various pH*caffeine combinations. At all sampling times, pH significantly affected all the biological variables considered, whereas caffeine exhibited a significant influence at T2 only. Among the various hemocyte parameters, caffeine caused a significant increase in total hemocyte count at T2, and in hemocyte volume and diameter at both T1 and T2, when a significant interaction between pH and caffeine was also found. Overall, results demonstrated that hemocyte functionality was strongly influenced by the experimental conditions tested. Further studies are needed to assess combined effects of climate changes and emerging contaminants on bivalve immune system when challenged with environmental pathogens.

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“…[13][14][15][16] A large number of researches have reported the ecotoxicity of CBZ on aquatic organisms, for instance, oxidative stress, neurotoxic effects, and genotoxic effects. 17,18 Cu is a widely spread metal in aquatic system, whose concentrations detected in aquatic environment are within the low ppb range, for example, 0.6 to 4.2 μg/L in the Mediterranean coastal wetland, 5 1 to 100 μg/L in the ocean waters, 19 and 20.9 to 72.4 μg/L in the Huelva estuary system. 20 Excessive exposure of Cu can lead to the elevated excretion of reactive oxygen species (ROS), resulting in the oxidative stress even oxidative damage.…”

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confidence: 99%

Single and combined effects of carbamazepine and copper on nervous and antioxidant systems of zebrafish (Danio rerio)

Jia

et al. 2020

Environmental Toxicology

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Various pollutants co‐exist in the aquatic environment such as carbamazepine (CBZ) and copper (Cu), which can cause complex effects on inhabiting organisms. The toxic impacts of the single substance have been studied extensively. However, the studies about their combined adverse impacts are not enough. In the present study, zebrafish were exposed to environmental relevant concentrations of CBZ (1, 10, and 100 μg/L), Cu (0.5, 5, and 10 μg/L) and the mixtures (1 μg/L CBZ + 0.5 μg/L Cu, 10 μg/L CBZ + 5 μg/L Cu, 100 μg/L CBZ + 10 μg/L Cu) for 45 days, the effects on nervous and antioxidant systems of zebrafish were investigated. The results demonstrated that, in comparison with single exposure group, the combined presence of CBZ and Cu exacerbated the effect of antioxidant system (the ability of inhibition of hydroxyl radicals (IHR), superoxide dismutase (SOD), catalase (CAT) and glutathione S‐transferase (GST)) but not nervous system (Acetylcholinesterase [AChE]). The qPCR results supported the changes of corresponding enzymes activities. Hepatic histopathological analysis verified the results of biomarkers. Our work illustrated that the toxicity of mixed pollutants is very complicated, which cannot simply be inferred from the toxicity of single pollutant, and calls for more co‐exposure experiments to better understanding of the co‐effects of pollutants on aquatic organisms.

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General stress, detoxification pathways, neurotoxicity and genotoxicity evaluated in Ruditapes philippinarum exposed to human pharmaceuticals

Cited by 116 publications

References 111 publications

Effects of single and combined exposure of pharmaceutical drugs (carbamazepine and cetirizine) and a metal (cadmium) on the biochemical responses of R. philippinarum

Effects of single and combined exposure of pharmaceutical drugs (carbamazepine and cetirizine) and a metal (cadmium) on the biochemical responses of R. philippinarum

Exposure to Decreased pH and Caffeine Affects Hemocyte Parameters in the Mussel Mytilus galloprovincialis

Single and combined effects of carbamazepine and copper on nervous and antioxidant systems of zebrafish (Danio rerio)

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