General Principles in the Diagnosis of Infection

Kradin, Richard L.; Iafrate, A. John

doi:10.1016/b978-0-323-44585-6.00002-3

Cited by 4 publications

(8 citation statements)

References 20 publications

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“…Erregernachweise können zum einen zytologisch mittels kornealer Abstrich-/Abradatpräparate sowie zum anderen mit spezielleren histochemischen Färbungen wie Gomori-Methenamin-Versilberung [GMS], Ziehl-Neelsen, Gram oder immunhistochemischen Färbungen geführt werden 9 , 11 , 12 , 13 , 14 . In der allgemeinen Pathologie werden daneben routinemäßig molekularpathologische Methoden zur Suche von Bakterien oder Pilzen eingesetzt (bspw.…”

Section: Diagnostikmethoden Der Pathologieunclassified

“…Es kann dabei zu qualitativen Einschränkungen der Morphologie kommen. Die ultrastrukturelle Diagnostik kann zum Nachweis von Viren, Mikrosporidien und auch Akanthamöben herangezogen werden 14 , 15 , 16 , 17 , 18 .…”

Section: Diagnostikmethoden Der Pathologieunclassified

“…Pathogen detection can be carried out cytologically on the one hand, by means of corneal swab/abradate preparations, and on the other hand with more specific histochemical staining such as Gomori-methenamine silvering [GMS], Ziehl-Neelsen, Gram or immunohistochemical staining 9 , 11 , 12 , 13 , 14 . In general pathology, molecular pathology methods are also routinely used to search for bacteria or fungi (for example for Helicobacter pylori, “fungal PCR”).…”

Section: Diagnostic Pathology Methodsmentioning

confidence: 99%

“…Qualitative limitations of the morphology can occur. Ultrastructural diagnostics can be used to detect viruses, microsporidia, and acanthamoebae as well 14 , 15 , 16 , 17 , 18 .…”

Section: Diagnostic Pathology Methodsmentioning

confidence: 99%

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Zur klinisch-pathologischen Korrelation der mikrobiellen Keratitis und darüber hinaus: Gibt es eine korneale Sepsis?

Nissen

Saeger

Nölle

et al. 2022

Klin Monbl Augenheilkd

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ZusammenfassungMikrobielle, infektiöse, Keratitiden bedingen relevant die Indikation von perforierenden Keratoplastiken. Durch eine akute transplantationsbedürftige Situation ergibt sich eine histopathologische Untersuchung der gesamten Korneadicke. Wenn auch die klinische Diagnose einer infektiösen Keratitis dabei nicht immer zu belegen ist, kann in der Pathologie sehr zur diagnostischen Klärung des klinischen Befundes und der Pathogenese beigetragen werden. Dies gelingt mit dem Einsatz vielfältiger Methoden aus Zytologie, Histochemie, Immunhistologie, Molekularpathologie und selten eingesetzter Elektronenmikroskopie, wodurch es möglich ist, geweblich manifestierte Vor- und Begleiterkrankungen nachzuweisen und die infrage kommenden Erreger anzugeben. Lässt sich eine klinisch-pathologische Korrelation für die Korneaschädigung nicht zufriedenstellend erbringen, stellt sich die Frage, ob ein mutmaßlicher Erreger final gar nicht dafür verantwortlich gewesen ist. Die Pathogenese einer transplantationsbedürftigen Keratitis ist auch experimentell am Menschen bisher nicht vollständig entschlüsselt. Die Entwicklung einer derartigen Keratitis kann zu einer klinischen Symptomatik führen, die auch als „bedrohliche Organdysfunktion“ beschrieben wird, ein Begriff aus der Sepsisforschung. Unter Berücksichtigung aktueller Literatur werden mögliche Übereinstimmungen zwischen Sepsis und mikrobieller Keratitis und ihr Bezug zur Histopathologie diskutiert.

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Section: Diagnostikmethoden Der Pathologieunclassified

Section: Diagnostic Pathology Methodsmentioning

confidence: 99%

“…Qualitative limitations of the morphology can occur. Ultrastructural diagnostics can be used to detect viruses, microsporidia, and acanthamoebae as well 14 , 15 , 16 , 17 , 18 .…”

Section: Diagnostic Pathology Methodsmentioning

confidence: 99%

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Zur klinisch-pathologischen Korrelation der mikrobiellen Keratitis und darüber hinaus: Gibt es eine korneale Sepsis?

Nissen

Saeger

Nölle

et al. 2022

Klin Monbl Augenheilkd

View full text Add to dashboard Cite

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“…Its precision is defined by the selection of specific primers that will hybridize only with the nucleic acid molecule of interest, while its sensitivity has been proven to effectively detect and amplify as low as one copy of target DNA molecule [22,23]. Since its invention in 1985, the PCR technique and PCR-based detection systems have been extensively studied for biomedical research [24][25][26][27][28], forensic studies [29,30], environmental analysis [31][32][33][34][35], food industries [36,37], and even at the international space station for extraterrestrial experimentation [38]. Although this method is more sensitive and less time consuming than its microorganismculture counterpart, it still requires multiple hours, materials, and equipment to be completed.…”

Section: Introductionmentioning

confidence: 99%

PCR-assisted impedimetric biosensor for colibactin-encoding pks genomic island detection in E. coli samples

et al. 2021

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A fast PCR-assisted impedimetric biosensor was developed for the selective detection of the clbN gene from the polyketide synthase (pks) genomic island in real Escherichia coli samples. This genomic island is responsible for the production of colibactin, a harmful genotoxin that has been associated with colorectal cancer. The experimental protocol consisted of immobilizing the designated forward primer onto an Au electrode surface to create the sensing probe, followed by PCR temperature cycling in blank, positive, and negative DNA controls. Target DNA identification was possible by monitoring changes in the system's charge transfer resistance values (R ct ) before and after PCR treatment through electrochemical impedance spectroscopy (EIS) analysis. Custom-made, flexible gold electrodes were fabricated using chemical etching optical lithography. A PCR cycle study determined the optimum conditions to be at 6 cycles providing fast results while maintaining a good sensitivity. EIS data for the DNA recognition process demonstrated the successful distinction between target interaction resulting in an increase in resistance to charge transfer (R ct ) percentage change of 176% for the positive DNA control vs. 21% and 20% for the negative and non-DNA-containing controls, respectively. Results showed effective fabrication of a fast, PCR-based electrochemical biosensor for the detection of pks genomic island with a calculated limit of detection of 17 ng/μL.

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Efficient Removal of Basic Fuchsin from Synthetic Medical Wastewater and Competitive Adsorption in the Mixture

Ahmed

Salihi

Kaufhold

et al. 2023

Adsorption Science & Technology

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The Ziehl-Neelsen stain is a mixture of Basic Fuchsin (BF), phenol (Ph), and methylene blue. It is used to stain the cell walls of Mycobacterium species. In this study, Basic Fuchsin was efficiently removed from synthetic wastewater using natural clay of Gankawa (GC) from Sulaimanyah city, and the effect of the presence of high concentrations of phenol in the adsorption mixture is demonstrated. In addition, X-ray diffraction (XRD), X-ray fluorescence (XRF), N2 gas adsorption analyzer, and field-emission scanning electron microscopy (FESEM) were used to characterize the natural clay. The clay was found to be mostly calcite, with a minor percentage of smectite, and contaminated with low percentages of illite. The adsorption kinetics show a relatively fast equilibration time (60-70 minutes). A second-order pseudokinetic model better fits the experimental kinetic data. The effect of the initial pH of the solution mixture was negligible at the experimental concentration range of the study. Freundlich and Langmuir’s adsorption isotherm models were applied to the equilibrium experimental data using nonlinear regression curve fitting. Both kinetics and isotherm studies point to a chemical adsorption mechanism for the process. For adsorption in the mixture, phenol molecules were found to compete with BF molecules for the active adsorption sites, while a synergetic effect of BF exists on phenol adsorption. As a naturally abundant cheap material, GC shows a superior adsorption capacity toward BF (287.0 mg g-1) over all natural materials and most of the synthetic or modified materials found in the literature.

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General Principles in the Diagnosis of Infection

Cited by 4 publications

References 20 publications

Zur klinisch-pathologischen Korrelation der mikrobiellen Keratitis und darüber hinaus: Gibt es eine korneale Sepsis?

Zur klinisch-pathologischen Korrelation der mikrobiellen Keratitis und darüber hinaus: Gibt es eine korneale Sepsis?

PCR-assisted impedimetric biosensor for colibactin-encoding pks genomic island detection in E. coli samples

Efficient Removal of Basic Fuchsin from Synthetic Medical Wastewater and Competitive Adsorption in the Mixture

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