General Preparation and Staining of Thin Sections

Roland, Jean-Claude; Vian, B.

doi:10.1016/b978-0-12-318880-9.50006-5

Cited by 146 publications

(107 citation statements)

References 31 publications

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“…However, this test did not permit discrimination between the wall and de\"eiopingpits. Identification of the material deposited around developing pits as callose is consistent with its amorphous electron-transparent appearance and lack of reactivity to PATAg staining (Roland, 1978). This is in line with the notion that liverworts are able to produce callose not only in the cells of the sporogenous lineage (Brown & Lemmon, 1987) but also in somatic cells (Lerchl et al, 1989).…”

Section: Wall Heterogeneity and The Origin Of The Pitssupporting

confidence: 63%

Development of water‐conducting cells in the antipodal liverwort Symphyogyna brasiliensis (Metzgeriales)

Ligrone¹,

Duckett

1996

New Phytologist

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SUMMARYThe thallus of the metzgerialean liverwort Symphyogyna brasiliensis Nees contains a strand of dead thick-walled cells with helicoidally-arranged pits that are presumably involved in water transport. During the first phase of differentiation these cells undergo a 13-16-fold elongation while remaining thin-walled and almost unchanged in diameter. During suhsequent maturation the walls hecome strongly thickened hy deposition of highly electronopaque material on extraplasmodesmal areas and of transparent material forming collars around plasmodesmata. Whilst the growing wall shows an ordered microfihrillar texture and is strongly reactive to PATAg staining for carbohydrates, the material associated with plasmodesmata is amorphous and PATAg-negative. A dense cortical array of microtohules (MTs) overlies the growing wall except in proximity to plasmodesmata, which are closely associated with tuhular endoplasmic reticulum (ER). During cellular maturation plasmodesmata undergo extensive secondary elongation hy incorporation of cortical ER supposedly continuous with desmotuhules. Quantitative analysis of plasmodesmal frequencies in relation to cellular elongation and wall thickness indicates that there is no de novo formation of plasmodesmata. Cortical MTs, wall microfibrils and secondarily-modified piasmodesmata are consistently co-aligned, all forming helices of about 45°. During maturation the Golgi apparatus proliferates and a vast number of vesicles containing PATAg-positive material are produced from a membrane domain interpreted as trans Golgi network, whilst PATAg-negative vesicles are formed along the fenestrated margins of cis and medial dictyosomal cisternae. Exocytosis of PATAg-positive vesicles is confined to extraplasmodesmal areas. In ageing cells abundant fibrillar material, also positive to PATAg-test, accumulates within pleomorphic membrane-bounded tubules. Final cytoplasmic dissolution involves the lysis of all cellular membranes and the liberation of the membrane-bounded fihrillar material, that is subsequently deposited onto the walls. The eventual dissolution of the plugs of amorphous electron-transport material results in the formation of open pits. Similarities in the cytological mechanisms underlying pore development in water-conducting cells of Symphyogvna and in the sieve elements of angiosperms are discussed.

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Section: Wall Heterogeneity and The Origin Of The Pitssupporting

confidence: 63%

Development of water‐conducting cells in the antipodal liverwort Symphyogyna brasiliensis (Metzgeriales)

Ligrone¹,

Duckett

1996

New Phytologist

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“…After rinsing, the anthcrs were subjected to a Ferric Hydroxylamin test (Roland 1978) to detect csterified pectins. -OsO1: fixation (24 h) with 1% 0sO-l in veronal acetate buffer pII 7,2.…”

Section: Methodsmentioning

confidence: 99%

Electron Microscope Evidence for a Membrane Around the Core of the Ubisch Body inLilium(Liliaceae)

Clément

Audran

1993

Grana

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“…Leaves were randomly sampled from three pots for each species and chamber, and prepared using the glutaraldehyde/osmium/critical point drying technique according to Roland & Vian (1991). The dried specimens were coated with gold and examined using SEM (Philips Model 500).…”

Section: Semmentioning

confidence: 99%

Effects of O₃ on the stomatal behaviour of Egyptian varieties of radish (Raphanus sativus L. cv. Baladey) and turnip (Brassica rapa L. cv. Sultani)

1994

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SUMMARYThe effects of ()., (80 nl I"') on the photosynthetic rate (A) and stomatal conductance (gj of Egyptian varieties of radish [Raplianus sativus L, cv, Baladey) and turnip (Brassica rapa L, c\ , Sultani) were examined. Exposure to Od ecreased A in both species, while g. was increased in radish and decreased in turnip. The effect of different concentrations of C, (50, 100, 150 nl 1"') on radish was examined and it was found that A decreased, while gî ncreased, with increasing concentrations of O^j.Scanning electron microscopy (SEM) of fumigated leaves showed that the increase in g^ in radish resulted from the collapse of epidermal cells. Counts of the percentage of stomata which were fully open demonstrated that 80 nl r^ O^ caused an increase of 18 "o in radish and a decrease of 14 "u in turnip. The proportion of opened stomata was found to increase with increasing O^ concentration in radish.

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General Preparation and Staining of Thin Sections

Cited by 146 publications

References 31 publications

Development of water‐conducting cells in the antipodal liverwort Symphyogyna brasiliensis (Metzgeriales)

Development of water‐conducting cells in the antipodal liverwort Symphyogyna brasiliensis (Metzgeriales)

Electron Microscope Evidence for a Membrane Around the Core of the Ubisch Body inLilium(Liliaceae)

Effects of O₃ on the stomatal behaviour of Egyptian varieties of radish (Raphanus sativus L. cv. Baladey) and turnip (Brassica rapa L. cv. Sultani)

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General Preparation and Staining of Thin Sections

Cited by 146 publications

References 31 publications

Development of water‐conducting cells in the antipodal liverwort Symphyogyna brasiliensis (Metzgeriales)

Development of water‐conducting cells in the antipodal liverwort Symphyogyna brasiliensis (Metzgeriales)

Electron Microscope Evidence for a Membrane Around the Core of the Ubisch Body inLilium(Liliaceae)

Effects of O3 on the stomatal behaviour of Egyptian varieties of radish (Raphanus sativus L. cv. Baladey) and turnip (Brassica rapa L. cv. Sultani)

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Effects of O₃ on the stomatal behaviour of Egyptian varieties of radish (Raphanus sativus L. cv. Baladey) and turnip (Brassica rapa L. cv. Sultani)