General Pathology of the Peripheral Joints

Gardner, D L

doi:10.1016/b978-0-12-655102-0.50014-x

Cited by 9 publications

References 242 publications

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Structure of newly synthesised (³⁵S)‐proteoglycans and (³⁵s)‐proteoglycan turnover products of cartilage explant cultures from dogs with experimental osteoarthritis

Carney

Billingham²,

Muir

et al. 1985

Journal Orthopaedic Research

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The structure of newly synthesised proteoglycans from explant cultures of cartilage from joints subjected to transection of the anterior cruciate ligament (osteoarthritic) and from normal (non- or sham-operated) joints was examined. The structure of the products of proteoglycan turnover was also examined using explants of normal and osteoarthritic cartilage maintained in culture for a 48 h chase period. The findings were as follows: Newly synthesised (35S)-proteoglycans extracted from cartilage explants from osteoarthritic joints whether examined 3 weeks, 3 months, or 6 months after surgery were larger than those from corresponding normal cartilage. This can be explained by the synthesis in osteoarthritic cartilage of abnormally long chondroitin sulphate chains on newly synthesised proteoglycans. The extracts also contained a newly formed small proteoglycan species that was unable to interact with hyaluronic acid. The proportion of this species was higher in osteoarthritic cartilage compared with normal, examined 3 weeks after surgery, but was generally absent from cartilage obtained 3 and 6 months after surgery. Compared with controls, a smaller proportion of the (35S)-proteoglycans released into the maintenance medium of explant cultures of osteoarthritic cartilage during a 48 h chase period was able to interact with hyaluronic acid. However, although furnished with longer (35S)-glycosaminoglycan chains, these proteoglycans were smaller than those from control explants.

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Structure of newly synthesised (³⁵S)‐proteoglycans and (³⁵s)‐proteoglycan turnover products of cartilage explant cultures from dogs with experimental osteoarthritis

Carney

Billingham²,

Muir

et al. 1985

Journal Orthopaedic Research

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Production of split patterns on the articular cartilage surfaces of rats

OʼConnor

Bland

Gardner

et al. 1980

The Journal of Pathology

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A device has been designed to permit reproducible loads to be applied perpendicularly to the acutely curved cartilage surfaces of the diarthrodial joints of small animals. With this apparatus, a sharp, round pin has been used to cause splits in the hyaline articular cartilage of the lower end of the femurs of 37 Wistar rats. In the patellar groove, the splits form a longitudinal pattern. On the condylar surface, the pattern is fantail, radial or non-uniform. It is suggested that cartilage thickness, joint congruence and load per unit area, are variables related to the directional pattern of the splits. These variables, in turn, reflect the gross, microscopic and molecular anatomy of the cartilage. The effects pin-pricks are comples: they disrupt surface collagen bundles and other cartilage components. Experiments made to test the effects of drying, isotonic saline, hyaluronic acid and sodium hydroxide support the view that the state of hydration and fluid flow are important determinants of the shape of cartilage splits. However, sodium hydroxide-induced cartilage disruption, involving glycosaminoglycan loss, did not influence split configuration. Some preliminary evidence suggests that the process of crack propagation, by which splits form, may be related to the position and shape of chondrocyte lacunae. It is concluded that pin-prick testing offers a means by which the structural properties of the cartilage of small diarthrodial joints may be conveniently tested in vitro.

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Experimental Models of Arthritis

B¹,

Spanel²,

Haferkamp³

1982

Current Topics in Pathology

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General Pathology of the Peripheral Joints

Cited by 9 publications

References 242 publications

Structure of newly synthesised (³⁵S)‐proteoglycans and (³⁵s)‐proteoglycan turnover products of cartilage explant cultures from dogs with experimental osteoarthritis

Structure of newly synthesised (³⁵S)‐proteoglycans and (³⁵s)‐proteoglycan turnover products of cartilage explant cultures from dogs with experimental osteoarthritis

Production of split patterns on the articular cartilage surfaces of rats

Experimental Models of Arthritis

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General Pathology of the Peripheral Joints

Cited by 9 publications

References 242 publications

Structure of newly synthesised (35S)‐proteoglycans and (35s)‐proteoglycan turnover products of cartilage explant cultures from dogs with experimental osteoarthritis

Structure of newly synthesised (35S)‐proteoglycans and (35s)‐proteoglycan turnover products of cartilage explant cultures from dogs with experimental osteoarthritis

Production of split patterns on the articular cartilage surfaces of rats

Experimental Models of Arthritis

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Structure of newly synthesised (³⁵S)‐proteoglycans and (³⁵s)‐proteoglycan turnover products of cartilage explant cultures from dogs with experimental osteoarthritis

Structure of newly synthesised (³⁵S)‐proteoglycans and (³⁵s)‐proteoglycan turnover products of cartilage explant cultures from dogs with experimental osteoarthritis