General odorant-binding proteins and sex pheromone guide larvae of Plutella xylostella to better food

Zhu, Jianqing; Ban, Liping; Song, Lingling; Liu, Yang; Pelosi, Paolo; Wang, Guirong

doi:10.1016/j.ibmb.2016.03.005

Cited by 97 publications

(113 citation statements)

References 46 publications

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“…In particular, the gene encoding GOBP2 is expressed in larval antenna, where it might bind pheromone cues. Such hypothesis was originated from what was observed in Plutella xylostella [70]. However, for all PBP genes we could not find their expression in H .…”

Section: Discussionmentioning

confidence: 64%

Candidate odorant binding proteins and chemosensory proteins in the larval chemosensory tissues of two closely related noctuidae moths, Helicoverpa armigera and H. assulta

Chang

Zhang

et al. 2017

PLoS ONE

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In order to acquire enough nutrients and energy for further development, larvae need to invest a large portion of their sensory equipments to identify food sources. Yet, the molecular basis of odor-driven behavior in larvae has been poorly investigated. Information on olfactory genes, particularly odorant binding proteins (OBPs) and chemosensory proteins (CSPs) which are involved in the initial steps of olfaction is very scarce. In this study, we have identified 26 OBP and 21 CSP genes from the transcriptomes of Helicoverpa armigera larval antennae and mouthparts. A comparison with the 34 OBP and 18 CSP genes of the adult antenna, revealed four novel OBPs and seven novel CSPs. Similarly, 27 OBPs (six novel OBPs) and 20 CSPs (6 novel CSPs) were identified in the transcriptomes of Helicoverpa assulta larval antennae and mouthparts. Tissue-specific profiles of these soluble proteins in H. armigera showed that 6 OBP and 4 CSP genes are larval tissue-specific, 15 OBPs and 13 CSPs are expressed in both larvae and adult, while the rest are adult- specific. Our data provide useful information for functional studies of genes involved in larval foraging.

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Section: Discussionmentioning

confidence: 64%

Candidate odorant binding proteins and chemosensory proteins in the larval chemosensory tissues of two closely related noctuidae moths, Helicoverpa armigera and H. assulta

Chang

Zhang

et al. 2017

PLoS ONE

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“…Based on these previous researches, it is possible to conclude that the OBPs we identify within our current study (i.e., those that are expressed in nonolfactory tissues) may have other physiological or chemosensory functions. Here we propose that these proteins may participate in other chemosensory functions, such as the modulation of gustation, as opposed to (or in conjunction with) any role in olfaction (Blomquist and Vogt, 2003;Leal, 2005;Ling et al, 2014;Gonzalez-Gonzalez et al, 2016;Zhu et al, 2016). The additional OBPs we identify that are abundantly or specifically expressed in the B. dorsalis head may also function in olfaction because the maxillary palps were not explicitly removed in our experiments.…”

Section: Discussionmentioning

confidence: 91%

An Antennae-Specific Odorant-Binding Protein Is Involved in Bactrocera dorsalis Olfaction

Zhao

Liang

et al. 2020

Front. Ecol. Evol.

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Insect antennae are important olfactory organs that house high concentrations of odorant-binding proteins (OBPs) in the sensillum lymph. Previous studies in other insects have shown that OBPs play important roles in transporting odorants and enhancing the sensitivity of the olfactory system. However, the functions of OBPs in the oriental fruit fly, Bactrocera dorsalis, especially those specifically expressed in antennae, have not been fully elucidated. In this study, cDNA libraries were constructed from both the male and female antennal transcriptome, and twenty OBPs were identified in total. The expression profiles of these OBPs were examined in the adult antenna, head, thorax, leg, and abdomen of both sexes. Seven of the identified OBP genes had significantly higher expression in both the male and female antennae than in other tissues, while the transcript levels of the remaining OBPs varied across different tissues. Regarding the function of antenna-specific OBPs, we targeted Bdorsobp2 as a representative for further RNA interference (RNAi) and identified via electrophysiology a decrease in detection of a potential species-specific a potent attractant, methyl eugenol. Moreover, subsequent behavioral assay data showed that the behavioral response of B. dorsalis toward this odorant decreased when Bdorobp2 was silenced with injection of double-stranded RNA (dsRNA). Combined, these results support our initial hypothesis that antennae-specific OBPs are of critical importance for insect odorant detection, sensitivity, and behavior.

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“…e-mail: juergen.krieger@zoologie.uni-halle.de By definition, sex pheromones are chemical compounds that serve sexual interaction between adult male and female individuals of the same species and thus are important parameters for reproductive behaviour. In recent studies on Spodoptera littoralis, S. exigua and Plutella xylostella, it was unexpectedly found that the larvae (regardless of sex and stage) also respond to the main sex pheromone component and are attracted to food containing this species-specific chemical signal (Poivet et al, 2012;Jin et al, 2015;Zhu et al, 2016). However, it is unclear how these compounds are received by the larvae, ie whether the larvae employ the same, highly specific molecular elements as found in OSNs of the male antenna, especially the elements that determine pheromone specificity, most notably the receptors for pheromone components and the putative co-receptor SNMP1.…”

Section: Introductionmentioning

confidence: 99%

Larval sensilla of the moth Heliothis virescens respond to sex pheromone components

Zielonka

Gehrke

Badeke

et al. 2016

Insect Molecular Biology

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Female-released sex pheromones orchestrate the mating behaviour of moths. Recent studies have shown that sex pheromones not only attract adult males but also caterpillars. Single sensillum recordings revealed that larval antennal sensilla of the moth Heliothis virescens respond to specific sex pheromone components. In search for the molecular basis of pheromone detection in larvae, we found that olfactory sensilla on the larval antennae are equipped with the same molecular elements that mediate sex pheromone detection in adult male moths, including the Heliothis virescens receptors 6 (HR6) and HR13, as well as sensory neurone membrane protein 1 (SNMP1). Thirty-eight olfactory sensory neurones were identified in three large sensilla basiconica; six of these are considered as candidate pheromone responsive cells based on the expression of SNMP1. The pheromone receptor HR6 was found to be expressed in two cells and the receptor HR13 in three cells. These putative pheromone responsive neurones were accompanied by cells expressing pheromone-binding protein 1 (PBP1) and PBP2. The results indicate that the responsiveness of larval sensilla to female-emitted sex pheromones is based on the same molecular machinery as in the antennae of adult males.

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General odorant-binding proteins and sex pheromone guide larvae of Plutella xylostella to better food

Cited by 97 publications

References 46 publications

Candidate odorant binding proteins and chemosensory proteins in the larval chemosensory tissues of two closely related noctuidae moths, Helicoverpa armigera and H. assulta

Candidate odorant binding proteins and chemosensory proteins in the larval chemosensory tissues of two closely related noctuidae moths, Helicoverpa armigera and H. assulta

An Antennae-Specific Odorant-Binding Protein Is Involved in Bactrocera dorsalis Olfaction

Larval sensilla of the moth Heliothis virescens respond to sex pheromone components

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