GENERAL MICROBIOLOGY OF <i>recA</i>: Environmental and Evolutionary Significance

Miller, Robert V.; Kokjohn, Tyler A.

doi:10.1146/annurev.mi.44.100190.002053

Cited by 193 publications

(147 citation statements)

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“…Conserved regions include domains thought to be involved with nucleotide binding (14) and single-stranded DNA binding (28). As is the case with alignments of gram-negative bacterial recA sequences to one another (22), gene conservation is weakest at the extreme 3' end of the coding region. One unusual feature of the A. laidlawii protein is that it lacks the acidic carboxyl terminus typical of RecA proteins (25).…”

Section: G E Q a L D I A E A L I K S Gs 1 Dm I V Imentioning

confidence: 97%

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Cloning and DNA sequence of a mycoplasmal recA gene

Dybvig

Woodard

1992

J Bacteriol

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Mycoplasmas are wall-less prokaryotes phylogenetically related to gram-positive bacteria. In order to investigate DNA recombination in these organisms, we MATERIALS AND METHODSStrains, bacteriophages, plasmids, and media. The strains, bacteriophages, and plasmids used are listed in Table 1. All organisms were grown at 37°C. A. laidlawii, Bacillus subtilis, and E. coli were grown in mycoplasma medium (7), brain heart infusion broth (Difco Laboratories, Detroit, Mich.), and LB medium (32), respectively. Phage lysates were prepared by induction of lysogenic strains ofB. subtilis with 0.5 ,ug of mitomycin C (Sigma Chemical Co., St. Louis, Mo.) per ml, as previously described (20). Plasmids were maintained in E. coli by using 50 ,ug of ampicillin per ml or 1,000 jxg of erythromycin per ml for selection.DNA isolation. Mycoplasmal DNA was isolated as described elsewhere (6). Plasmid DNA was isolated from E. coli by using the alkaline lysis method (32) and further purified by CsCI-ethidium bromide density gradient centrifugation (32). A generous supply of purified phage 4lO5Rec4il DNA, given to us by R. E. Yasbin, was sufficient for most of the experiments described here. However, we isolated additional j105Rec4il DNA as well as phage 41i05J23 DNA by phenol extraction of purified phage particles, as previously described (20

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Section: G E Q a L D I A E A L I K S Gs 1 Dm I V Imentioning

confidence: 97%

“…There are several pathways of homologous recombination in Escherichia coli, all of which require the recA gene product (22,25). Although little is known about DNA recombination in mycoplasmas, homologous recombination has been demonstrated in the species Mycoplasma pulmonis (18).…”

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confidence: 99%

Cloning and DNA sequence of a mycoplasmal recA gene

Dybvig

Woodard

1992

J Bacteriol

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“…The SOS response in Bacillus subtilis progresses in a similar manner, with B. subtilis RecA having an identical role in controlling the SOS regulon together with a cellular repressor protein that is functionally homologous to the E. coli LexA repressor (Wojciechowski et al, 1991). SOS-like processes have been conserved in a wide variety of eubacterial species (Miller & Kokjohn, 1990;Roca & Cox, 1990). On the one hand, RecA is the sole protein required for LexA1 (DRA0344) cleavage, although LexA1 is not involved in RecA induction in D. radiodurans (Narumi et al, 2001;Bonacossa de Almeida et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Down-regulation of radioresistance by LexA2 in Deinococcus radiodurans

et al. 2006

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The extremely radioresistant bacterium Deinococcus radiodurans contains two LexA homologues (LexA1 and LexA2) that are possible transcriptional regulators associated with the DNA damage response. In this study, resequencing revealed that there was an additional cytosine nucleotide (nucleotide position 612) in the D. radiodurans lexA2 gene. Purified LexA2 possessed proteolytic activity that could be stimulated by RecA. In an effort to gain an insight into the role of LexA2 in the radiation response mechanism, recA, lexA1 and lexA2 disruptant strains were generated and investigated. The intracellular level of RecA increased in lexA1 and lexA2 disruptant strains following γ-irradiation as in the wild-type strain. These results indicated that the two LexA homologues did not possess functional overlap regarding the induction of RecA. The lexA2 disruptant strains exhibited a much higher resistance to γ-rays than the wild-type strain. Furthermore, a luciferase assay showed that pprA promoter activation was enhanced in the lexA2 disruptant strain following γ-irradiation. The pprA gene encoding the novel radiation-inducible protein PprA plays a critical role in the radioresistance of D. radiodurans. The increase in radioresistance of the lexA2 disruptant strain is explained in part by the enhancement of pprA promoter activation.

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“…Similar DNA repair systems have been reported in other bacteria (Miller & Kokjohn, 1990). In this way, the regulatory genes of the SOS system, l e x A and recA, of different Gram-positive and Gram-negative bacteria have been characterized (Raymond-Denise & Guillen, 1991;Garriga et af., 1992;Roca & Cox, 1997).…”

Section: Introductionmentioning

confidence: 53%

Determination of the Paracoccus denitrificans SOS box

Rey¹,

Diestra²,

Henestrosa³

et al. 1999

Microbiology

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By gel retardation experiments with crude cell extracts of Paracoccus denitrificans it was demonstrated that a protein specifically binds to the promoter Of the P. denitrifiCanS recA gene. PCR mUtageneSiS Of the r e d promoter showed that the GAACN,GAAC motif is required for the formation of the DNA-protein complex. This protein also binds to the GTTCN,GTTC motif, which is present in the promoter of the P. denitrificans uvrA gene. Mutational analysis of the promoter regions of both P. denitrificans recA and uwrA genes indicated that the GAACN,GAAC and GTTCN,GTTC sequences are required for DNA-damage-mediated induction of these two genes in vivo. Furthermore, the P. denitrificans recA gene was DNA-damage-inducible when introduced into cells of the phylogenetically related phototrophic bacterium Rhodobacter sphaeroides, although this inducibility was lost in mutants in the GAACN,GAAC motif. These results indicate that P. denitrificans possesses the same SOS box as R. sphaeroides, which, in agreement with previous work, is proposed as being the GTTCN,GTTC motif. 1

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GENERAL MICROBIOLOGY OF recA: Environmental and Evolutionary Significance

Cited by 193 publications

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Cloning and DNA sequence of a mycoplasmal recA gene

Cloning and DNA sequence of a mycoplasmal recA gene

Down-regulation of radioresistance by LexA2 in Deinococcus radiodurans

Determination of the Paracoccus denitrificans SOS box

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