General Immunology of the Genital Tract

Weissenbacher, E. R.; Wirth, Monika; Mylonas, Ioannis; Geburtshilfe, Klinik für; Ledger, William J.

doi:10.1007/978-3-642-14906-1_3

Cited by 2 publications

(2 citation statements)

References 219 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Adaptive immunity is characterized by identification of specific antigen determinants of pathogens and the ability to distinguish one microorganism from another. B-lymphocytes produce specific antibodies, while T-lymphocytes have multiple functions such as to assist in antibody production and participate in the cell mediated defense [179]. Ultimately this response is efficient and leads to direct elimination of the pathogen in most cases.…”

Section: Adaptive Immune Defensementioning

confidence: 99%

Expression of Psoriasin in Human Papillomavirus–Induced Cervical High-Grade Squamous Intraepithelial Lesions

Alvendal

Kamolvit

Wagner

et al. 2019

J Low Genit Tract Dis

View full text Add to dashboard Cite

Background: Infections of the female genital tract are common and have severe impact on the quality of life and sexual health of affected women. The innate immune system is crucial and is involved in the defense of infections. Understanding innate immunity of the vaginal and cervical mucosa is important to enable development of future preventive and therapeutic strategies for genital infections. Objective: The aim of this thesis was to investigate innate immune factors in women with recurrent candida vulvovaginitis (RVVC) and high grade squamous intraepithelial lesions (HSIL) of the cervix, induced by human papilloma virus (HPV). This in order to contribute to the understanding of the innate immune response in these two common infections of the lower female genital tract. Material and Methods: Clinical examinations, measurements of intravaginal nitric oxide (NO) levels and vaginal biopsies were performed in 28 patients with RVVC and 31 healthy controls. Cervical biopsies and vaginal lavage were collected from 19 patients with HPV induced HSIL and 14 controls. Immunohistochemistry (IHC), enzyme-linked immunosorbent assay (ELISA), western blot and reverse transcriptase real time polymerase chain reaction (PCR)) were used to identify and quantify inducible nitric oxide synthase (iNOS), antimicrobial proteins, cytokines and encoding genes. Adhesion and binding assays were performed to evaluate the adhesive capacity of candida and to demonstrate the binding between candida and the antimicrobial protein psoriasin. Transmission electron microscopy was conducted to measure the cell wall thickness in C. albicans affected by psoriasin. To evaluate a new treatment strategy for RVVC, the effect of chlorhexidine digluconate and fluconazole on C. albicans eradication and biofilm was investigated in RVVC and commensal strains, using the crystal violet method and viable count. Results: NO levels were significantly higher in patients during acute infection compared to controls. Levels decreased after fluconazole treatment but remained higher than in controls. Furthermore, increased expression of iNOS was observed in the epithelial basal layer in patients both before and after treatment. There were positive correlations between NO levels, clinical symptoms and examination scores. Findings indicated that C. albicans induces production of psoriasin during mucosal candida infection. Psoriasin was shown to interact with β-glucan in the candida cell wall and inhibit candida adhesion to surfaces. In HPV induced HSIL, psoriasin expression and protein levels increased significantly after surgical treatment and reached similar levels as in controls. The mRNA expression of the proinflammatory cytokine IL-8 was higher before treatment and restored to the same levels as in controls six months after lesions were excised. Chlorhexidine digluconate prevented new biofilm formation and reduced already established C. albicans biofilm. Moreover, the number of candida cells in both planktonic state and within the biofilm were significantly decreased....

show abstract

Section: Adaptive Immune Defensementioning

confidence: 99%

Expression of Psoriasin in Human Papillomavirus–Induced Cervical High-Grade Squamous Intraepithelial Lesions

Alvendal

Kamolvit

Wagner

et al. 2019

J Low Genit Tract Dis

View full text Add to dashboard Cite

show abstract

“…IL-10 actively suppresses the maternal immune system to avoid rejection of the fetal allograft (Thaxton and Sharma, 2010;Cheng and Sharma, 2015). On the other hand, IL-18 may stimulate the production of interferon-gamma, which is needed for the remodeling of the blood vessels during pregnancy (Weissenbacher et al, 2014). Given the importance of these interleukins in pregnancy, a number of studies have investigated the association between polymorphisms in interleukin genes and the risk of recurrent pregnancy loss.…”

Section: Introductionmentioning

confidence: 99%

Association between IL-6 -174G/C Polymorphism and unexplained Recurrent Abortion among Pregnant Ladies

Mahmoud Abdelateif,

Elradi Ali Elradi

2024

oiuj

View full text Add to dashboard Cite

Objective: The aim of this study was to determine the association between interleukin 6 (IL-6 -174G/C) with susceptibility to Recurrent abortion (RA) in Sudanese pregnant ladies. Methods: case control study, the genetic polymorphism of IL-6 -174G/C were studied by SSP- PCR in the DNA of a total of Forty (30) Sudanese ladies with the history of abortion and (30) ladies with normal pregnancy to three or more birth and without a previous abortion in the first trimester. Statistical analysis was performed using Chi square, correlation to spearman test to set differences were considered statistically significant with a p<0.05. Results: CG genotyping prevalence were significantly higher in cases than in controls, P= 0.01, respectively. On the other hand, GG genotype prevalence were higher among control than in RM patients, P = 0.01, respectively. Conclusions: This study has proven a possible association of IL-6 −174 G/C polymorphism with susceptibility recurrent abortions.

show abstract

General Immunology of the Genital Tract

Cited by 2 publications

References 219 publications

Expression of Psoriasin in Human Papillomavirus–Induced Cervical High-Grade Squamous Intraepithelial Lesions

Expression of Psoriasin in Human Papillomavirus–Induced Cervical High-Grade Squamous Intraepithelial Lesions

Association between IL-6 -174G/C Polymorphism and unexplained Recurrent Abortion among Pregnant Ladies

Contact Info

Product

Resources

About