The fish fauna of Doon valley, part of District Dehradun was studied for a period of 2 years from March, 2010 -February, 2012. 56 species belonging to 5 Orders, 13 Families and 30 Genera were collected from various sampling stretches set on 5 rivers viz., Baldi, Song, Suswa in the East and Tons and Asan in the West. Of them, 6 are the new records viz., Puntius terio (Hamilton), Lepidocephalichthys annandalei Chaudhuri, Colisa lalia (Hamilton), Colisa labiosus (Day), Channa marulius (Hamilton) and Channa harcourtbutleri (Annandale). Family Cyprinidae was the most dominating family contributing 53% (30 species) to the fish fauna of Doon Valley, followed by Balitoridae and Channidae, both contributing 7% each to the percentage, represented by 4 species each. Cyprinus carpio (river Song, S 7 ) and Clarius gariepinnus (river Tons, S 15 ) are accidental catches of exotic species. As per the fishbase.org status (2007), 52 species (92.85%) are adjudged native, 2 species (3.57%) endemic and 2 species (3.57%) introduced. On the other hand, the IUCN (2015 -4) status outlines that 41 species are Least Concern, 3 Vulnerable, 1 Endangered, 3 Near Threatened, and 8 Not Assessed.