GENERAL CONTROL NONREPRESSIBLE4 Degrades 14-3-3 and the RIN4 Complex to Regulate Stomatal Aperture with Implications on Nonhost Disease Resistance and Drought Tolerance

Kaundal, Amita; Vemanna, Ramu S.; Oh, Sunhee; Lee, Seong-Hee; Pant, Bikram Datt; Lee, Hee-Kyung; Rojas, Clemencia M.; Senthil‐Kumar, Muthappa; Mysore, Kirankumar S.

doi:10.1105/tpc.17.00070

Cited by 58 publications

(59 citation statements)

References 65 publications

(85 reference statements)

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“…We also found that cotton RPM1‐interacting protein 4 (RIN4) (CotAD_42472) and 14‐3‐3 protein (CotAD_64613) are putative substrates of the GhMORG1‐dependent GhMKK6‐GhMPK4 cascade, which is involved in regulating the resistance of cotton to F. oxysporum (Figure ). It was recently reported that RIN4 interacts with GCN4 (an AAA + ‐ATPase) and 14‐3‐3 proteins and regulates plasma membrane H + ‐ATPase activity in Arabidopsis (Kaundal et al , ). The overexpression of GCN4 resulted in the degradation of both RIN4 and 14‐3‐3 proteins via the proteasome pathway and reduced the stomatal response to fusicoccin (a fungal toxin produced by the fungus Fusicoccum amygdali ) (Kaundal et al , ).…”

Section: Discussionmentioning

confidence: 99%

“…It was recently reported that RIN4 interacts with GCN4 (an AAA + ‐ATPase) and 14‐3‐3 proteins and regulates plasma membrane H + ‐ATPase activity in Arabidopsis (Kaundal et al , ). The overexpression of GCN4 resulted in the degradation of both RIN4 and 14‐3‐3 proteins via the proteasome pathway and reduced the stomatal response to fusicoccin (a fungal toxin produced by the fungus Fusicoccum amygdali ) (Kaundal et al , ). These results suggested that the GhMORG1‐mediated MAPK cascade affected the cotton response to F. oxysporum by regulating plasma membrane H + ‐ATPase activity.…”

Section: Discussionmentioning

confidence: 99%

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Scaffold protein GhMORG1 enhances the resistance of cotton to Fusarium oxysporum by facilitating the MKK6‐MPK4 cascade

Wang

Guo

et al. 2019

Plant Biotechnology Journal

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In eukaryotes, MAPK scaffold proteins are crucial for regulating the function of MAPK cascades. However, only a few MAPK scaffold proteins have been reported in plants, and the molecular mechanism through which scaffold proteins regulate the function of the MAPK cascade remains poorly understood. Here, we identified GhMORG1, a GhMKK6-GhMPK4 cascade scaffold protein that positively regulates the resistance of cotton to Fusarium oxysporum. GhMORG1 interacted with GhMKK6 and GhMPK4, and the overexpression of GhMORG1 in cotton protoplasts dramatically increased the activity of the GhMKK6-GhMPK4 cascade. Quantitative phosphoproteomics was used to clarify the mechanism of GhMORG1 in regulating disease resistance, and thirty-two proteins were considered as the putative substrates of the GhMORG1dependent GhMKK6-GhMPK4 cascade. These putative substrates were involved in multiple disease resistance processes, such as cellular amino acid metabolic processes, calcium ion binding and RNA binding. The kinase assays verified that most of the putative substrates were phosphorylated by the GhMKK6-GhMPK4 cascade. For functional analysis, nine putative substrates were silenced in cotton, respectively. The resistance of cotton to F. oxysporum was decreased in the substrate-silenced cottons. These results suggest that GhMORG1 regulates several different disease resistance processes by facilitating the phosphorylation of GhMKK6-GhMPK4 cascade substrates. Taken together, these findings reveal a new plant MAPK scaffold protein and provide insights into the mechanism of plant resistance to pathogens.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Scaffold protein GhMORG1 enhances the resistance of cotton to Fusarium oxysporum by facilitating the MKK6‐MPK4 cascade

Wang

Guo

et al. 2019

Plant Biotechnology Journal

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“…Blue light-induced stomatal opening is mediated through activation of plasma membrane H + -ATPases in guard cells (Yamauchi et al, 2016). Moreover, stomata must open and close in response to various environmental stimuli, including light, humidity, and foliar pathogens (Roelfsema and Hedrich, 2005;Shimazaki et al, 2007;Shope et al, 2008;Jezek and Blatt, 2017;Kaundal et al, 2017). Presumably, membrane traffic and function of primary transporters of guard cells, such as the plasma membrane H + -ATPases and the KAT1 K + channels, must be tightly regulated in response to multiple environmental stimuli and stresses.…”

Section: Syp132 Affects the Physiology Of Plant Growth And Stomatal Rmentioning

confidence: 99%

Unusual Roles of Secretory SNARE SYP132 in Plasma Membrane H⁺-ATPase Traffic and Vegetative Plant Growth

et al. 2019

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The plasma membrane proton (H +)-ATPases of plants generate steep electrochemical gradients and activate osmotic solute uptake. H +-ATPase-mediated proton pumping orchestrates cellular homeostasis and is a prerequisite for plastic cell expansion and plant growth. All evidence suggests that the population of H +-ATPase proteins at the plasma membrane reflects a balance of their roles in exocytosis, endocytosis, and recycling. Auxin governs both traffic and activation of the plasma membrane H +-ATPase proteins already present at the membrane. As in other eukaryotes, in plants, SNARE-mediated membrane traffic influences the density of several proteins at the plasma membrane. Even so, H +-ATPase traffic, its relationship with SNAREs, and its regulation by auxin have remained enigmatic. Here, we identify the Arabidopsis (Arabidopsis thaliana) Qa-SNARE SYP132 (Syntaxin of Plants132) as a key factor in H +-ATPase traffic and demonstrate its association with endocytosis. SYP132 is a low-abundant, secretory SNARE that primarily localizes to the plasma membrane. We find that SYP132 expression is tightly regulated by auxin and that augmented SYP132 expression reduces the amount of H +-ATPase proteins at the plasma membrane. The physiological consequences of SYP132 overexpression include reduced apoplast acidification and suppressed vegetative growth. Thus, SYP132 plays unexpected and vital roles in auxin-regulated H +-ATPase traffic and associated functions at the plasma membrane.

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“…Nicotiana benthamiana , a member of the Solanaceae family, is a model plant species that is widely used for studying host–pathogen interactions and for transient protein expression to examine protein function, subcellular protein localization, and protein–protein interactions (Anand et al., ; Chakravarthy, Velásquez, Ekengren, Collmer, & Martin, ; Gilbert & Wolpert, ; Goodin, Zaitlin, Naidu, & Lommel, ; Kaundal et al., ; Lee et al., ; Liu et al., ; Rojas et al., ; Wang et al., 2012). In addition to these uses, N. benthamiana is also an attractive model to study the function of genes involved in abiotic stress responses, plant development, and metabolism (Chakravarthy et al., ; Gas‐Pascual, Berna, Bach, & Schaller, ; Jones, Keining, Eamens, & Vaistij, ; Liu et al., ; Ramegowda, Senthil‐kumar, Udayakumar, & Mysore, ; Senthil‐Kumar, Lee, & Mysore, ).…”

Section: Introductionmentioning

confidence: 99%

Virus‐induced gene silencing database for phenomics and functional genomics in Nicotiana benthamiana

et al. 2018

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Virus‐induced gene silencing (VIGS) is an important forward and reverse genetics method for the study of gene function in many plant species, especially Nicotiana benthamiana. However, despite the widespread use of VIGS, a searchable database compiling the phenotypes observed with this method is lacking. Such a database would allow researchers to know the phenotype associated with the silencing of a large number of individual genes without experimentation. We have developed a VIGS phenomics and functional genomics database (VPGD) that has DNA sequence information derived from over 4,000 N. benthamiana VIGS clones along with the associated silencing phenotype for approximately 1,300 genes. The VPGD has a built‐in BLAST search feature that provides silencing phenotype information of specific genes. In addition, a keyword‐based search function could be used to find a specific phenotype of interest with the corresponding gene, including its Gene Ontology descriptions. Query gene sequences from other plant species that have not been used for VIGS can also be searched for their homologs and silencing phenotype in N. benthamiana. VPGD is useful for identifying gene function not only in N. benthamiana but also in related Solanaceae plants such as tomato and potato. The database is accessible at http://vigs.noble.org.

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GENERAL CONTROL NONREPRESSIBLE4 Degrades 14-3-3 and the RIN4 Complex to Regulate Stomatal Aperture with Implications on Nonhost Disease Resistance and Drought Tolerance

Cited by 58 publications

References 65 publications

Scaffold protein GhMORG1 enhances the resistance of cotton to Fusarium oxysporum by facilitating the MKK6‐MPK4 cascade

Scaffold protein GhMORG1 enhances the resistance of cotton to Fusarium oxysporum by facilitating the MKK6‐MPK4 cascade

Unusual Roles of Secretory SNARE SYP132 in Plasma Membrane H⁺-ATPase Traffic and Vegetative Plant Growth

Virus‐induced gene silencing database for phenomics and functional genomics in Nicotiana benthamiana

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GENERAL CONTROL NONREPRESSIBLE4 Degrades 14-3-3 and the RIN4 Complex to Regulate Stomatal Aperture with Implications on Nonhost Disease Resistance and Drought Tolerance

Cited by 58 publications

References 65 publications

Scaffold protein GhMORG1 enhances the resistance of cotton to Fusarium oxysporum by facilitating the MKK6‐MPK4 cascade

Scaffold protein GhMORG1 enhances the resistance of cotton to Fusarium oxysporum by facilitating the MKK6‐MPK4 cascade

Unusual Roles of Secretory SNARE SYP132 in Plasma Membrane H+-ATPase Traffic and Vegetative Plant Growth

Virus‐induced gene silencing database for phenomics and functional genomics in Nicotiana benthamiana

Contact Info

Product

Resources

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Unusual Roles of Secretory SNARE SYP132 in Plasma Membrane H⁺-ATPase Traffic and Vegetative Plant Growth