General Control Nonrepressed Protein 5 Modulates Odontogenic Differentiation Through NF-κB Pathway in Tumor Necrosis Factor-α-Mediated Impaired Human Dental Pulp Stem Cells

Xiao, Jingwen; Zheng, Ya; Zhang, Wei; Zhang, Ye; Cao, Peipei; Lv, Yi; Bao, Liuliu; Shi, S; Feng, Xingmei

doi:10.1089/cell.2021.0113

Cited by 2 publications

(1 citation statement)

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“…Similarly, the inflammatory milieu in the periodontal region hinders stem cell-based alveolar bone regeneration. Levels of various pro-inflammatory cytokines including TNF-α are upregulated in the pulpitis-affected pulp tissue and periodontitis-affected alveolar bone [51,52]. TNF-α is frequently used in vitro cell cultures as an inflammatory cytokine mimicking the inflammatory situation in vivo [53].…”

Section: Discussionmentioning

confidence: 99%

Cannabidiol Rescues TNF-α-Inhibited Proliferation, Migration, and Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells

Zeng

Zhang

et al. 2023

Biomolecules

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Strategies to promote dental pulp stem cells (DPSCs) functions including proliferation, migration, pro-angiogenic effects, and odontogenic/osteogenic differentiation are in urgent need to restore pulpitis-damaged dentin/pulp regeneration and DPSCs-based bone tissue engineering applications. Cannabidiol (CBD), an active component of Cannabis sativa has shown anti-inflammation, chemotactic, anti-microbial, and tissue regenerative potentials. Based on these facts, this study aimed to analyze the effect of CBD on DPSCs proliferation, migration, and osteogenic/odontogenic differentiation in basal and inflammatory conditions. Highly pure DPSCs with characteristics of mesenchymal stem cells (MSCs) were successfully isolated, as indicated by the results of flowcytometry and multi-lineage (osteogenic, adipogenic, and chondrogenic) differentiation potentials. Among the concentration tested (0.1–12.5 µM), CBD (2.5 μM) showed the highest anabolic effect on the proliferation and osteogenic/odontogenic differentiation of DPSCs. Pro-angiogenic growth factor VEGF mRNA expression was robustly higher in CBD-treated DPSCs. CBD also prompted the migration of DPSCs and CBD receptor CB1 and CB2 expression in DPSCs. TNF-α inhibited the viability, migration, and osteogenic/odontogenic differentiation of DPSCs and CBD reversed these effects. CBD alleviated the TNF-α-upregulated expression of pro-inflammatory cytokines TNF-α, interleukin (IL)-1β, and IL-6 in DPSCs. In conclusion, our results indicate the possible application of CBD on DPSCs-based dentin/pulp and bone regeneration.

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Section: Discussionmentioning

confidence: 99%