General and Cross-Pathway Controls of Amino Acid Biosynthesis

Sachs, Murray B.

doi:10.1007/978-3-662-10367-8_14

Cited by 41 publications

(59 citation statements)

References 222 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is maintained in prnA ¹ strains and highlighted by the 3AT inhibition experiments. This control system is probably under a CpcA-like protein as it is lost in a mutant where the only canonical CpcA (Hinnebusch, 1988;Sachs, 1996) putative binding element present in the prnD-prnB intergenic region is mutated. The putative, CpcA-responsive cis-acting amino acid starvation-responsive element was mutated from 5Ј-TTGACTCAG-3Ј to 5Ј-TAGACTCAG-3Ј.…”

Section: Discussionmentioning

confidence: 99%

“…The analogue 3-amino-1,2,4-triazole (3AT) has been shown to lead to histidine starvation through inhibition of histidine biosynthesis both in S. cerevisiae and Neurospora crassa (Hinnebusch, 1988;Sachs and Yanofsky, 1991;Sachs, 1996). We compared the induction of prnB, prnC, and prnD by proline and by 3AT in mycelia of prnA þ and prnA ¹ strains (Fig.…”

Section: Proline Transport Is Activated During Conidiospore Germinationmentioning

confidence: 99%

“…We then investigated whether a factor homologous to GCN4 of S. cerevisiae or CPC1 of N. crassa (Hinnebusch, 1984;Thireos et al, 1984;Hinnebusch, 1988;Paluh et al, 1988;Sachs, 1996) could be involved in this phenomenon. However, a GCN4/cpc-1 homologue has not been cloned in A. nidulans and was only recently described in Aspergillus niger (Wanke et al, 1997).…”

Section: Proline Transport Is Activated During Conidiospore Germinationmentioning

confidence: 99%

See 2 more Smart Citations

The gene encoding the major proline transporter of Aspergillus nidulans is upregulated during conidiospore germination and in response to proline induction and amino acid starvation

Tazebay

Sophianopoulou

Scazzocchio

et al. 1997

Molecular Microbiology

View full text Add to dashboard Cite

SummaryIn Aspergillus nidulans a highly specific L-proline transporter is encoded by the prnB gene which is tightly linked to all other genes involved in proline catabolism. In mycelia, the expression of the prn structural genes is finely co-regulated in response to proline induction and nitrogen/carbon catabolite repression. In this study we establish that prnB expression is also activated during germination of conidiospores. This activation persists until the development of 6 h-old mycelia and it is independent of proline induction mediated by the pathway-specific prnA gene product. We then show that, in mycelia, prnB transcription is activated in response to proline or histidine starvation. This process has two components: a prnA-dependent and a prnA-independent component. A cis-acting element that conforms to the consensus target of the GCN4/CPC1 transcriptional activators mediating amino acid biosynthesis activation in other fungi is involved in the activation of prnB transcription in response to amino acid starvation. We also show that the stimulation of prnB expression in germinating conidiospores is not due exclusively to transient internal amino acid starvation occurring during the transition from conidiospore to mycelium. This is the first report that an amino acid transporter gene is upregulated during development and in response to amino acid starvation and specific amino acid induction.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Proline Transport Is Activated During Conidiospore Germinationmentioning

confidence: 99%

Section: Proline Transport Is Activated During Conidiospore Germinationmentioning

confidence: 99%

See 1 more Smart Citation

The gene encoding the major proline transporter of Aspergillus nidulans is upregulated during conidiospore germination and in response to proline induction and amino acid starvation

Tazebay

Sophianopoulou

Scazzocchio

et al. 1997

Molecular Microbiology

View full text Add to dashboard Cite

show abstract

“…Fungal amino acid biosynthetic genes and their products are subject to multiple levels of control (12,26,29,30,48). We examined translational and transcriptional modulation of the expression of two genes involved in Neurospora crassa amino acid biosynthesis, arg-2 and cpc-1.…”

mentioning

confidence: 99%

“…The arg-2 gene specifies the small subunit of arginine-specific carbamoyl phosphate synthetase (CPS-A) (41) and was among the first metabolic genes identified (58). cpc-1 specifies a transcriptional activator of amino acid biosynthetic genes that positively regulates gene expression in response to amino acid starvation; it resembles Saccharomyces cerevisiae GCN4 and mammalian c-jun (43,48).…”

mentioning

confidence: 99%

Translational Regulation in Response to Changes in Amino Acid Availability in Neurospora crassa

Luo

Freitag

Sachs

1995

Molecular and Cellular Biology

View full text Add to dashboard Cite

We examined the regulation of Neurospora crassa arg-2 and cpc-1 in response to amino acid availability. arg-2 encodes the small subunit of arginine-specific carbamoyl phosphate synthetase; it is subject to unique negative regulation by Arg and is positively regulated in response to limitation for many different amino acids through a mechanism known as cross-pathway control. cpc-1 specifies a transcriptional activator important for crosspathway control. Expression of these genes was compared with that of the cytochrome oxidase subunit V gene, cox-5. Analyses of mRNA levels, polypeptide pulse-labeling results, and the distribution of mRNA in polysomes indicated that Arg-specific negative regulation of arg-2 affected the levels of both arg-2 mRNA and arg-2 mRNA translation. Negative translational effects on arg-2 and positive translational effects on cpc-1 were apparent soon after cells were provided with exogenous Arg. In cells limited for His, increased expression of arg-2 and cpc-1, and decreased expression of cox-5, also had translational and transcriptional components. The arg-2 and cpc-1 transcripts contain upstream open reading frames (uORFs), as do their Saccharomyces cerevisiae homologs CPA1 and GCN4. We examined the regulation of arg-2-lacZ reporter genes containing or lacking the uORF start codon; the capacity for arg-2 uORF translation appeared critical for controlling gene expression.

show abstract

Cross-Pathway and Pathway-Specific Control of Amino Acid Biosynthesis inMagnaporthe grisea

Shen

Ebbole

1997

Fungal Genetics and Biology

View full text Add to dashboard Cite

General and Cross-Pathway Controls of Amino Acid Biosynthesis

Cited by 41 publications

References 222 publications

The gene encoding the major proline transporter of Aspergillus nidulans is upregulated during conidiospore germination and in response to proline induction and amino acid starvation

The gene encoding the major proline transporter of Aspergillus nidulans is upregulated during conidiospore germination and in response to proline induction and amino acid starvation

Translational Regulation in Response to Changes in Amino Acid Availability in Neurospora crassa

Cross-Pathway and Pathway-Specific Control of Amino Acid Biosynthesis inMagnaporthe grisea

Contact Info

Product

Resources

About