Gene trap screening as an effective approach for identification of Wnt‐responsive genes in the mouse embryo

Yamaguchi, Yoshifumi; Ogura, Satoshi; Ishida, Mami; Karasawa, Mika; Takada, Shinji

doi:10.1002/dvdy.20348

Cited by 11 publications

(20 citation statements)

References 76 publications

(87 reference statements)

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“…Grainyhead-related transcription factor is required for duct maturation in the salivary gland and the kidney of the mouse As in the case of these grainyhead family genes, Cp2l1 is also expressed in epithelium in a characteristic pattern: in the developing duct epithelium of the salivary gland and kidney in the mouse embryo (Rodda et al, 2001;Yamaguchi et al, 2005). Interestingly, we found that Cp2l1 is expressed in the developing epithelial ducts of other organs.…”

Section: Introductionmentioning

confidence: 48%

“…During the course of gene-trap mutagenesis (Yamaguchi et al, 2005), we found that Cp2l1 is expressed in the epithelium of the developing salivary (submandibular, sublingual and parotid) glands ( Fig. 1A-D) and kidney ( Fig.…”

Section: Cp2l1 Expression In Fluid/solute-transporting Epithelial Tismentioning

confidence: 99%

“…The production of the Cp2l1 trap mice has been reported previously (Yamaguchi et al, 2005). These mice were backcrossed into a C57BL/6 background for six to ten generations and then analyzed.…”

Section: Mouse Strainsmentioning

confidence: 99%

“…X-gal staining was performed as described previously (Yamaguchi et al, 2005). Briefly, fresh cryosections were fixed in 1% formaldehyde or 2% PFA for 5 to 10 minutes, washed twice with phosphate-buffered saline, and stained for lacZ activity.…”

Section: X-gal Staining and In Situ Hybridizationmentioning

confidence: 99%

“…X-gal-stained sections were fixed with 4% PFA for 10 minutes and then processed for in situ hybridization. (Nakai et al, 2003); Cp2l1 (Yamaguchi et al, 2005); Pax2 (gift from P. Gruss); and ␤-NGF, Sgn1 and Mist1 (gifts from S. Yoshida) (Yoshida et al, 2001). The cDNAs of Fxyd2 variant b, Fxyd2 variant c, Scnn1b, Slc34a1 and Slc4a1 were cloned by PCR using the primers described in Table 1.…”

Section: X-gal Staining and In Situ Hybridizationmentioning

confidence: 99%

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Grainyhead-related transcription factor is required for duct maturation in the salivary gland and the kidney of the mouse

Yamaguchi

Yonemura²,

Takada

2006

Development

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Duct epithelial structure is an essential feature of many internal organs, including exocrine glands and the kidney. The ducts not only mediate fluid transfer but also help to maintain homeostasis. For instance, fluids and solutes are resorbed from or secreted into the primary fluid flowing through the lumen of the ducts in the exocrine glands and kidneys. The molecular mechanism underlying the functional maturation of these ducts remains largely unknown. Here, we show that a grainyhead-related transcription factor, CP2-like 1 (CP2L1), is required for the maturation of the ducts of the salivary gland and kidney. In the mouse, Cp2l1 is specifically expressed in the developing ducts of a number of exocrine glands, including the salivary gland, as well as in those of the kidney. In Cp2l1-deficient mice, the expression of genes directly involved in functional maturation of the ducts was specifically reduced in both the salivary gland and kidney, indicating that Cp2l1 is required for the differentiation of duct cells. Furthermore, the composition of saliva and urine was abnormal in these mice. These results indicate that Cp2l1 expression is required for normal duct development in both the salivary gland and kidney.

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Section: Introductionmentioning

confidence: 48%

Section: Cp2l1 Expression In Fluid/solute-transporting Epithelial Tismentioning

confidence: 99%

Section: Mouse Strainsmentioning

confidence: 99%

Section: X-gal Staining and In Situ Hybridizationmentioning

confidence: 99%

Section: X-gal Staining and In Situ Hybridizationmentioning

confidence: 99%

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Grainyhead-related transcription factor is required for duct maturation in the salivary gland and the kidney of the mouse

Yamaguchi

Yonemura²,

Takada

2006

Development

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Molecular cloning, sequence characterization and recombinant expression of Nanog gene in goat fibroblast cells using lentiviral based expression system

Singhal

Malik

et al. 2014

Mol Biol Rep

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Nanog is a homeodomain containing protein which plays important roles in regulation of signaling pathways for maintenance and induction of pluripotency in stem cells. Because of its unique expression in stem cells it is also regarded as pluripotency marker. In this study goat Nanog (gNanog) gene has been amplified, cloned and characterized at sequence level with successful over-expression in CHO-K1 cell line using a lentiviral based system. gNanog ORF is 903 bp long which codes for Nanog protein of size 300 amino acids (aas). Complete nucleotide sequence shows some evolutionary mutation in goat in comparision to other species. Protein sequence of goat is highly similar to other species. Overall, gNanog nucleotide sequence and predicted protein sequence showed high similarity and minimum divergence with cattle (96 % identity/4 % divergence) and buffalo (94/5 %) while low similarity and high divergence with pig (84/15 %), human (81/23 %) and mouse (69/40 %) indicating evolutionary closeness of gNanog to cattle and buffalo. gNanog lentiviral expression construct was prepared for over-expression of Nanog gene in adult goat fibroblast cells. Lentiviral expression construct of Nanog enabled continuous protein expression for induction and maintenance of pluripotency. Western blotting revealed the expression of Nanog gene at protein level which supported that the lentiviral expression system is highly promising for Nanog protein expression in differentiated goat cell.

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Olig2-positive progenitors in the embryonic spinal cord give rise not only to motoneurons and oligodendrocytes, but also to a subset of astrocytes and ependymal cells

Masahira

Takebayashi

Ono

et al. 2006

Developmental Biology

167

143

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Motoneurons and oligodendrocytes in the embryonic spinal cord are produced from a restricted domain of the ventral ventricular zone, termed the pMN domain. The pMN domain is the site of expression of two basic helix-loop-helix transcription factors, Olig1 and Olig2, which are essential for motoneuron and oligodendrocyte development. Previous lineage-tracing experiments using Olig1-Cre and Olig2-GFP mice suggested that motoneurons and oligodendrocytes, but not astrocytes, are produced from the pMN domain. However, important questions remain, including the fate of neuroepithelial cells in the pMN domain, and specifically whether motoneurons and oligodendrocytes are the only types of cells produced in the pMN domain. We performed lineage-tracing experiments using a tamoxifen-inducible Cre-recombinase inserted into the Olig2 locus. We demonstrated that motoneurons and oligodendrocyte progenitors are derived from the Olig2+ progenitors in the pMN domain, and also found that a subset of astrocytes at the ventral surface of the spinal cord and ependymal cells at the ventricular surface are also produced from the pMN domain. These findings demonstrate that motoneurons and oligodendrocytes are not the only cell types originating from this domain.

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Gene trap screening as an effective approach for identification of Wnt‐responsive genes in the mouse embryo

Cited by 11 publications

References 76 publications

Grainyhead-related transcription factor is required for duct maturation in the salivary gland and the kidney of the mouse

Grainyhead-related transcription factor is required for duct maturation in the salivary gland and the kidney of the mouse

Molecular cloning, sequence characterization and recombinant expression of Nanog gene in goat fibroblast cells using lentiviral based expression system

Olig2-positive progenitors in the embryonic spinal cord give rise not only to motoneurons and oligodendrocytes, but also to a subset of astrocytes and ependymal cells

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