Gene transport and expression by arginine-rich cell-penetrating peptides in Paramecium

Dai, Yun-Hao; Liu, Betty Revon; Chiang, Hsin‐Ju; Lee, Han-Jung

doi:10.1016/j.gene.2011.08.011

Cited by 30 publications

(41 citation statements)

References 46 publications

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“…Accordingly, we have demonstrated that CPPs are able to efficiently deliver DNA (Chen et al, , 2012Dai et al, 2011a;Lee et al, 2011;Li et al, 2010) or RNA (Wang et al, 2007) into live cells or organisms. HR9 peptides are novel CPPs and were recently shown to deliver DNA at a higher efficiency than nona-arginine (R9) peptides did in both paramecia (Dai et al, 2011a) and insect cells (Chen et al, 2012). Further study indicated that the cellular entry for HR9/nanoparticle complexes is mediated by the direct membrane translocation pathway (Liu et al, 2011).…”

Section: Introductionmentioning

confidence: 90%

“…Recently, several studies have explored the possibility to apply CPPs as a nonviral gene delivery system (Bolhassani, 2011;Bolhassani et al, 2011;Chen et al, 2011;Dohmen and Wagner, 2011). Accordingly, we have demonstrated that CPPs are able to efficiently deliver DNA (Chen et al, , 2012Dai et al, 2011a;Lee et al, 2011;Li et al, 2010) or RNA (Wang et al, 2007) into live cells or organisms. HR9 peptides are novel CPPs and were recently shown to deliver DNA at a higher efficiency than nona-arginine (R9) peptides did in both paramecia (Dai et al, 2011a) and insect cells (Chen et al, 2012).…”

Section: Introductionmentioning

confidence: 94%

“…Three arginine-rich CPPs, SR9 (R9), HR9 (CH5-R9-H5C), and PR9 (FFLIPKG-R9), were synthesized as previously described (Chen et al, 2012;Dai et al, 2011a;Liu et al, 2011). HR9-FITC peptide containing the fluorescein isothiocyanate (FITC) at the N-terminus was chemically synthesized using solid-phase peptide synthesis and cross-linked with FITC by the FluoroTag FITC conjugation kit (Genomics, Taipei, Taiwan) according to the manufacturer's instructions (Sigma-Aldrich, Saint Louis, MO, USA).…”

Section: Preparation Of Plasmid Dna Peptide and Proteinmentioning

confidence: 99%

“…The transfection efficiency of the CPP-mediated gene delivery was determined as previously described (Chen et al, 2012;Dai et al, 2011a). The relative intensities of fluorescent images from the functional gene assay were converted and quantified using the UN-SCAN-IT software (Silk Scientific, Orem, UT, USA).…”

Section: Cpp-mediated Gene Delivery Into Human Cellsmentioning

confidence: 99%

See 3 more Smart Citations

Arginine-rich cell-penetrating peptides deliver gene into living human cells

Liu¹,

Lin²,

Chiang³

et al. 2012

Gene

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Section: Introductionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 94%

Section: Preparation Of Plasmid Dna Peptide and Proteinmentioning

confidence: 99%

Section: Cpp-mediated Gene Delivery Into Human Cellsmentioning

confidence: 99%

See 2 more Smart Citations

Arginine-rich cell-penetrating peptides deliver gene into living human cells

Liu¹,

Lin²,

Chiang³

et al. 2012

Gene

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“…10 Many studies have shown that modifying Arg in the conventional vector system increases cell-penetration efficiency, and increasing Arg concentration corresponds to enhanced transfection efficiency. 11,12 Since GABA is composed of four carbons, it can be used as a linker molecule. In our previous study, we showed that polymers coupled with a 6-carbon linker between PAMAM and Arg show higher transfection efficiency compared to native PAMAM dendrimers.…”

Section: Introductionmentioning

confidence: 99%

PAMAM Dendrimers Conjugated with L-Arginine and γ-Aminobutyric Acid as Novel Polymeric Gene Delivery Carriers

Son¹,

Yu²,

Choe³

et al. 2013

Bulletin of the Korean Chemical Society

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In this study, we synthesized functional dendrimer derivatives as nonviral gene delivery vectors. Poly(amidoamine) dendrimer (PAMAM, generation 4) was modified to possess functional amino acids to enhance gene transfection efficiency. PAMAM G4 derivatives conjugated with L-arginine (Arg) and γ-aminobutyric acid (GABA) showed higher transfection efficiency and lower cytotoxicity compared to the native PAMAM G4 dendrimer. The polyplex of the PAMAM G4 derivative/pDNA was evaluated using an agarose gel retardation assay and Picogreen reagent assay. Additionally, the MTT assay was performed to examine the cytotoxicity of synthesized polymers. All PAMAM G4 derivatives showed lower cytotoxicity than PEI25kD. Particularly, PAMAM G4-GABA-Arg displayed enhanced transfection efficiency compared to the native PAMAM G4 dendrimer.

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Gene and protein delivery using four cell penetrating peptides for HIV‐1 vaccine development

et al. 2019

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Cell penetrating peptides (CPPs) can potently transport therapeutic molecules to target cells for treatment of a variety of diseases. Thus, their use is critical to improve therapeutic vaccines. Histidine‐rich nona‐arginine (HR9) and primary amphipathic peptide (MPG) showed the ability to transfer DNA into the cells. Moreover, the peptide derived from the C‐terminal of the tumor suppressor protein p14ARF (M918) and arginine‐rich peptide (penetratin) were utilized to deliver polypeptides and proteins into the living cells. In this study, the immunostimulatory properties of HIV‐1 Nef DNA and protein constructs were evaluated using small heat shock protein 20 (sHsp20) and Freund's emulsion as an adjuvant, and four CPPs (HR9, MPG, M918, and penetratin) as a gene or protein carrier in BALB/c mice. Our data indicated that the HR9/DNA, MPG/DNA, M918/protein, and penetratin/protein complexes formed the stable nanoparticles that were effectively delivered in HEK‐293T cell line at certain ratios. Moreover, a heterologous Hsp20‐Nef DNA + MPG prime/rHsp20‐Nef protein+M918 boost regimen significantly elicited higher levels of IgG2a, IgG2b, IFN‐gamma, and Granzyme B directed toward Th1 responses in a long period (3 months) after the last immunization compared to other groups. Furthermore, the effective role of Hsp20 was detected as a natural adjuvant in enhancing immune responses against HIV‐1 Nef antigen. These findings demonstrated that the simultaneous use of M918 and MPG CPPs as protein and gene carriers improves HIV‐1 Nef‐specific B‐ and T‐cell immune responses as a promising approach for development of HIV‐1 monovalent vaccine.

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Gene transport and expression by arginine-rich cell-penetrating peptides in Paramecium

Cited by 30 publications

References 46 publications

Arginine-rich cell-penetrating peptides deliver gene into living human cells

Arginine-rich cell-penetrating peptides deliver gene into living human cells

PAMAM Dendrimers Conjugated with L-Arginine and γ-Aminobutyric Acid as Novel Polymeric Gene Delivery Carriers

Gene and protein delivery using four cell penetrating peptides for HIV‐1 vaccine development

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