Gene transfer to the rat biliary tract with the HVJ-cationic liposome method

Uehara, Tetsuya; Honda, Kazuo; Hatano, Etsuro; Terao, Ryuta; Iimuro, Yuji; Yamamoto, Naoki; Yamamoto, Masayuki; Kaneda, Yasufumi; Yamaoka, Yoshio

doi:10.1016/s0168-8278(99)80137-8

Cited by 15 publications

(13 citation statements)

References 26 publications

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“…The ß-galactosidase expression vector (pCAG-lacZ) was constructed as previously described [14]. HVJ cationic liposomes containing pCAG-lacZ (200 Ìg), previously incubated with 64 Ìg of a nuclear protein (the high mobility group 1 (HMG-1)), were prepared as described above for the HVJ cationic liposomes containing FITC-ODN.…”

Section: Preparation Of Hvj Cationic Liposomes Containing Pcag-lacz Amentioning

confidence: 99%

“…In this vector system, the HVJ promotes fusion of the liposome with the surface membrane of targeted cells resulting in delivery of DNA directly into the cell [13]. Uehara et al [14] reported that the HVJ cationic liposome method was successful for selective gene transfer to bile duct epithelial cells in vivo. On the other hand, Kupffer cells have never been selectively targeted, although direct injection of HVJ liposomes into the liver was performed [15].…”

Section: Introductionmentioning

confidence: 99%

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Kupffer Cell Targeting by Intraportal Injection of the HVJ Cationic Liposome

Yoshida¹,

Yamamoto²,

Uehara³

et al. 2002

Eur Surg Res

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The aim of this study was to target Kupffer cells (KCs) selectively and efficiently by the intraportal injection of fusigenic cationic liposomes with hemagglutinating virus of Japan components (HVJ cationic liposomes). Phosphorothioate FITC-oligodeoxynucleotides (FITC-ODNs) encapsulated in either HVJ cationic liposomes, HVJ anionic liposomes or conventional cationic liposomes without HVJ were transferred to the rat. FITC-ODNs in HVJ cationic liposomes administered via portal vein were selectively transfected to KCs for up to 24 h with no apparent cytotoxicity at higher transfection efficiency than FITC-ODNs in conventional cationic liposomes without HVJ administered via portal vein or tail vein. On the other hand, FITC-ODNs in HVJ anionic liposomes were observed mainly in hepatocytes, not KCs. This new method will be useful for the modulation of KCs activity in both basic research and clinical applications.

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Section: Preparation Of Hvj Cationic Liposomes Containing Pcag-lacz Amentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Kupffer Cell Targeting by Intraportal Injection of the HVJ Cationic Liposome

Yoshida¹,

Yamamoto²,

Uehara³

et al. 2002

Eur Surg Res

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“…The complex offers good transfection efficiency with reduced immunogenicity compared to other viral systems, thus enabling repeated administration. This carrier has been used to efficiently deliver plasmids to the liver by both the portal vein as well as the biliary system (Uehara et al, 1999;Hirano et al, 2001). The HVJ has recently been used in a successful preclinical tumor therapy animal study (Hasegawa et al, 2001).…”

Section: Manipulation Of Intracellular Distribution Of Exogenous Dnamentioning

confidence: 99%

In Vivo Application of Non-viral Vectors to the Liver

Richardson¹,

Kren²,

Steer³

2002

Journal of Drug Targeting

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The liver plays a central role in many inherited and acquired genetic disorders, and thus is a potential target for nucleic acid therapies. Despite the great strides made in basic molecular biology over the last two decades successful gene therapy remains elusive. Most recently, there has been considerable effort to develop non-viral gene therapy approaches, in part, to overcome the potential complications associated with viral delivery systems. This review outlines the different non-viral approaches available to the liver, and includes a detailed review of recent advances in delivery vectors for use in techniques such as gene augmentation, with particular emphasis on useful applications of antisense and ribozyme technology.

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“…b-Galactosidase expression in bile duct epithelial cells was observed 3 d after the administration of HVJ-cationic liposomes containing pCAG-lacZ through the papilla of Vater in a rat model [1]. Intrabiliary administration of the adenovirus vector of the retinoblastoma (RB) gene [2] and the p53 gene [3] inhibited epithelial and fibrous tissue proliferation in a cholangitis model of rats.…”

Section: Introductionmentioning

confidence: 99%