Gene Transfer into Mammalian Cells by Particle Bombardment

Delivery of Genes and Fluorescent Dyes into Cells of the Intact Lens by Particle Bombardment

“…A charge of compressed helium is used to accelerate the particles to velocities suf®cient to penetrate and transfect cells. Particle-mediated transfection has been employed in diverse experimental settings as an ef®cient transfection or immunization tool (Klein and Fitzpatrick-McElligott, 1993;Heiser, 1994). Within the eye, particle bombardment has recently been used to deliver genes to the cornea (Shiraishi et al, 1998) and retina .…”

Particle-mediated gene transfer into murine livers using a newly developed gene gun

“…It has been reported that compared with gene transfer by means of electroporation, lipofection and diethylaminoethyl dextran, particlemediated gene transfer could induce 50-to 240-fold higher levels of transgene expression in vitro. 23 Our results indicate that particle-mediated gene transfer into the liver appears to be much more promising than direct plasmid injection and electroporation-mediated gene transfer into the liver. Furthermore, particle-mediated gene transfer has the advantage of requiring a much lower amount of DNA compared with direct plasmid injection and electroporation-mediated gene transfer.…”

“…[16][17][18][19][20][21] Although various designs of gene gun instruments using helium gas have been described previously, all devices use macrocarriers for accelerating DNA-coated microcarriers. 22,23 Furthermore, since particle-mediated in vivo gene transfer has been performed mainly to epidermis, applications of this technology to visceral tissues have not been well investigated. In the present study, we used a newly developed gene gun instrument that uses a hammering bullet but not a macrocarrier, and examined the applicability of the gene gun to murine livers for in vivo gene therapy.…”

Transcriptional Regulation of Prostaglandin-H Synthase-2 Gene in Human Trophoblasts1

“…To test whether the observed effect of cAMP was mediated by PKA, we cotransfected primary trophoblasts with the PGHS-2 reporter construct, along with an expression vector for the catalytic subunit of PKA (16). As shown in Fig.…”