Gene Transfer in Plants: Production of Transformed Plants Using Ti Plasmid Vectors

Rogers, Stephen L.; Horsch, Robert B.; Fraley, Robert T.

doi:10.1016/b978-0-12-743655-5.50032-4

Cited by 61 publications

(59 citation statements)

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“…The plasmid pDR37 contains 1-6 copies of the TYLCV-Th B-DNA and has been described previously (Rochester et al, 1990). The plasmids used for agroinoculation were shuttled into Agrobacterium tumefaciens GV3111SE (Rogers et al, 1986). The modified strains ofA.…”

Section: Methodsmentioning

confidence: 99%

Complete nucleotide sequence of the geminivirus tomato yellow leaf curl virus, Thailand isolate

Rochester

DePaulo

Fauquet³

et al. 1994

Journal of General Virology

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Section: Methodsmentioning

confidence: 99%

Complete nucleotide sequence of the geminivirus tomato yellow leaf curl virus, Thailand isolate

Rochester

DePaulo

Fauquet³

et al. 1994

Journal of General Virology

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“…The PCR product was initially cloned into pGEM-T Easy vector, and then subcloned as an XbaI-SacI fragment into binary vector pBI121 to replace the GUS gene. The construct (35STGPAT5) was introduced into Agrobacterium tumefaciens strain C58C1 for Arabidopsis vacuum infiltration (Bechtold et al, 1993) and strain LBA4404 for tobacco leaf disc transformation (Rogers et al, 1986). Transgenic plants were then selected on Murashige and Skoog medium containing 50 mg mL 21 kanamycin (Murashige and Skoog, 1962).…”

Section: Cloning Of Gpat5 and Construction Of 35stgpat5 Overexpressiomentioning

confidence: 99%

Monoacylglycerols Are Components of Root Waxes and Can Be Produced in the Aerial Cuticle by Ectopic Expression of a Suberin-Associated Acyltransferase

et al. 2007

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The interface between plants and the environment is provided for aerial organs by epicuticular waxes that have been extensively studied. By contrast, little is known about the nature, biosynthesis, and role of waxes at the root-rhizosphere interface. Waxes isolated by rapid immersion of Arabidopsis (Arabidopsis thaliana) roots in organic solvents were rich in saturated C18-C22 alkyl esters of p-hydroxycinnamic acids, but also contained significant amounts of both a-and b-isomers of monoacylglycerols with C22 and C24 saturated acyl groups and the corresponding free fatty acids. Production of these compounds in root waxes was positively correlated to the expression of sn-glycerol-3-P acyltransferase5 (GPAT5), a gene encoding an acyltransferase previously shown to be involved in aliphatic suberin synthesis. This suggests a direct metabolic relationship between suberin and some root waxes. Furthermore, when ectopically expressed in Arabidopsis, GPAT5 produced very-longchain saturated monoacylglycerols and free fatty acids as novel components of cuticular waxes. The crystal morphology of stem waxes was altered and the load of total stem wax compounds was doubled, although the major components typical of the waxes found on wild-type plants decreased. These results strongly suggest that GPAT5 functions in vivo as an acyltransferase to a glycerol-containing acceptor and has access to the same pool of acyl intermediates and/or may be targeted to the same membrane domain as that of wax synthesis in aerial organs.

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“…These constructs were introduced into the A. tumefaciens strain LBA4404 (Hoekema et al, 1983) by direct transformation, as described by An et al (1988). Tobacco leaf discs were transformed as described by Rogers et al (1986).…”

Section: Biotin Carboxylase Plant Expression Constructs and Transformmentioning

confidence: 99%

Antisense Expression and Overexpression of Biotin Carboxylase in Tobacco Leaves

et al. 1997

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Although much is known about the biochemistry involved in the synthesis of fatty acids, comparatively little is understood about the regulation of this pathway. Recently, several-in vivo studies have implicated the plastidlocalized ACCase as a primary regulatory enzyme in fatty acid synthesis. Light activation of fatty acid synthesis in chloroplasts is controlled through the ACCase reaction (Post-Beittenmiller et al., 1991, 1992, and this enzyme was also identified as a regulatory step in the feedback inhibition of fatty acid synthesis in tobacco (Nicotiana tabacum) suspension cultures . Furthermore, Page et al. (1994) demonstrated that the plastid ACCase of maize (Zea muys L.) and barley (Hordeum vulgare) leaves exerts a high degree of flux control over fatty acid synthesis. These findings extend the observations from animal, fungal, and bacterial systems that implicate

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Gene Transfer in Plants: Production of Transformed Plants Using Ti Plasmid Vectors

Cited by 61 publications

References 0 publications

Complete nucleotide sequence of the geminivirus tomato yellow leaf curl virus, Thailand isolate

Complete nucleotide sequence of the geminivirus tomato yellow leaf curl virus, Thailand isolate

Monoacylglycerols Are Components of Root Waxes and Can Be Produced in the Aerial Cuticle by Ectopic Expression of a Suberin-Associated Acyltransferase

Antisense Expression and Overexpression of Biotin Carboxylase in Tobacco Leaves

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