Gene transfer efficiency of high primary amine content, hydrophobic, alkyl-oligoamine derivatives of polyethylenimine

Dehshahri, Ali; Oskuee, Reza Kazemi; Shier, W. Thomas; Hatefi, Arash; Ramezani, Mohammad

doi:10.1016/j.biomaterials.2009.04.036

Cited by 105 publications

(101 citation statements)

References 30 publications

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“…These observations are similar to the previously reported data by our group and others. 17,18 These results, in combination with the results of the transfection efficiency study, show that the optimum c/p ratio for cell transfection is 4. At this c/p ratio, at which all polymers are non-toxic, PEI-PEP6 has the highest rate of gene transfer efficiency.…”

Section: Characterization Of Pei-peptide/dna Complexesmentioning

confidence: 55%

“…This hypothesis was formulated on the basis of previous studies by Hatefi et al, 19 and intended to help us identify the most efficient KH peptide sequence(s). On the basis of the results obtained from this study and our previous findings, 17 we formulated our second hypothesis. It was hypothesized that by modification of the structure of alkyl-modified PEI 10 kDa with KH peptides, the transfection efficiency can be increased while maintaining low toxicity.…”

Section: Introductionmentioning

confidence: 74%

“…17 In brief, PEI 10 kDa (0.5 g) was dissolved in 5 ml dimethylformamide. 6-Bromohexanoic acid (0.88 g, 1.5 mol excess) was dissolved in dimethylformamide and added dropwise to the vigorously stirred PEI solution.…”

Section: Methodsmentioning

confidence: 99%

“…We have previously demonstrated that modification of PEI 10 kDa with 6-bromohexanoic acid enhances transfection efficiency while maintaining low toxicity. 17 Therefore, we used PEI-alkyl as the base and modified its structure by conjugation with PEP1, PEP2 and PEP6 to generate PEI-alkyl-PEP1, PEI-alkyl-PEP2 and PEI-alkyl-PEP6. We also evaluated the transfection efficiency and toxicity of PEI-alkyl-EDA modified with PEP1, PEP2 and PEP6.…”

Section: Characterization Of Pei-peptide/dna Complexesmentioning

confidence: 99%

“…Our group has recently reported the structure of a series of alkyl-oligoamine derivatives of PEI 10 kDa (that is, PEI-alkyl and PEI-alkyl-EDA) with enhanced efficiency due to increase in lipophilicity while maintaining low toxicity. 17,18 These derivatives of PEI 10 kDa have transfection efficiency comparable to that of PEI 25 kDa, but with significantly lower cell toxicity.…”

Section: Introductionmentioning

confidence: 98%

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Modified polyethyleneimine with histidine–lysine short peptides as gene carrier

et al. 2010

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There are several strategies that can be utilized to improve transfection efficiency while reducing the cytotoxicity of polyethyleneimine (PEI) as a promising non-viral gene delivery vector. In this study, we evaluated the potential use of lysinehistidine (KH) peptides in modifying the PEI 10 kDa structure and enhancing its efficiency while maintaining low toxicity of PEI. PEI 10 kDa was modified with 6-bromohexanoic acid (alkyl) to increase its lipophilicity. Then, ethylenediamine (EDA) was attached to the carboxylic groups of PEI-hexanoate to restore the primary amines of PEI. Subsequently, six different KH short peptides were conjugated to PEIs and evaluated for the effect of the KH sequence on vector transfection efficiency and cytotoxicity. The transfection efficiency of PEI-peptides complexed with a luciferase reporter gene (pRLCMV) in Neuro-2A murine neuroblastoma cells showed that the PEI conjugated to KHHHKKHHHK peptide had a significantly higher rate of gene transfection efficiency in comparison with other KH peptides. This peptide was conjugated to PEI-alkyl and PEI-alkyl-EDA and significant improvement in efficiency with minimal cytotoxicity was observed. The results obtained suggest that the sequence and content of KH peptides will have a significant impact on the transfection efficiency of modified PEI 10 kDa.

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Section: Characterization Of Pei-peptide/dna Complexesmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 74%

Section: Methodsmentioning

confidence: 99%

Section: Characterization Of Pei-peptide/dna Complexesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 3 more Smart Citations

Modified polyethyleneimine with histidine–lysine short peptides as gene carrier

et al. 2010

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Triazine Dendrimers for DNA and siRNA Delivery: Progress, Challenges, and Opportunities

Mintzer

Merkel

Kissel

et al. 2012

Dendrimer‐Based Drug Delivery Systems

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Delivering Colloidal Nanoparticles to Mammalian Cells: A Nano–Bio Interface Perspective

Verderio

Avvakumova

Alessio

et al. 2014

Adv Healthcare Materials

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Understanding the behavior of multifunctional colloidal nanoparticles capable of biomolecular targeting remains a fascinating challenge in materials science with dramatic implications in view of a possible clinical translation. In several circumstances, assumptions on structure-activity relationships have failed in determining the expected responses of these complex systems in a biological environment. The present Review depicts the most recent advances about colloidal nanoparticles designed for use as tools for cellular nanobiotechnology, in particular, for the preferential transport through different target compartments, including cell membrane, cytoplasm, mitochondria, and nucleus. Besides the conventional entry mechanisms based on crossing the cellular membrane, an insight into modern physical approaches to quantitatively deliver nanomaterials inside cells, such as microinjection and electro-poration, is provided. Recent hypotheses on how the nanoparticle structure and functionalization may affect the interactions at the nano-bio interface, which in turn mediate the nanoparticle internalization routes, are highlighted. In addition, some hurdles when this small interface faces the physiological environment and how this phenomenon can turn into different unexpected responses, are discussed. Finally, possible future developments oriented to synergistically tailor biological and chemical properties of nanoconjugates to improve the control over nanoparticle transport, which could open new scenarios in the field of nanomedicine, are addressed.

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Gene transfer efficiency of high primary amine content, hydrophobic, alkyl-oligoamine derivatives of polyethylenimine

Cited by 105 publications

References 30 publications

Modified polyethyleneimine with histidine–lysine short peptides as gene carrier

Modified polyethyleneimine with histidine–lysine short peptides as gene carrier

Triazine Dendrimers for DNA and siRNA Delivery: Progress, Challenges, and Opportunities

Delivering Colloidal Nanoparticles to Mammalian Cells: A Nano–Bio Interface Perspective

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