Gene transduction for disseminated intraperitoneal tumor using cationic liposomes containing non-histone chromatin proteins: cationic liposomal gene therapy of carcinomatosa

Namiki, Yoshihisa; Takahashi, Tadashi C.; Ohno, Tsuneya

doi:10.1038/sj.gt.3300577

Cited by 44 publications

(32 citation statements)

References 23 publications

(29 reference statements)

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“…20 However, when we conducted muscle transduction with plasmids or liposomes, serum level of SOD was not as high as that achieved in the present report (data not shown). In this context, further elucidation utilizing a cationic liposome, which is reportedly beneficial for muscle transduction, 21 may be needed. Nevertheless, we were able to achieve an increment of EC-SOD in the culture supernatant of transfectants with sufficient activity to suppress O − 2 -induced tumor cell motility.…”

Section: Discussionmentioning

confidence: 99%

Anti-metastatic gene therapy utilizing subcutaneous inoculation of EC-SOD gene transduced autologous fibroblast suppressed lung metastasis of Meth-A cells and 3LL cells in mice

et al. 2001

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We have previously reported that superoxide stimulates the motility of tumor cells and the administration of Cu-Zn superoxide dismutase (SOD) significantly suppresses metastasis. However, ideally, anti-metastatic therapy should be longlasting, systemically effective and have low toxicity. The halflife of Cu-Zn SOD in plasma is so short that it cannot provide long-lasting effects. Therefore, in this study we have developed a gene therapy in a mouse model utilizing extracellular SOD (EC-SOD), which is the most prevalent SOD

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Section: Discussionmentioning

confidence: 99%

Anti-metastatic gene therapy utilizing subcutaneous inoculation of EC-SOD gene transduced autologous fibroblast suppressed lung metastasis of Meth-A cells and 3LL cells in mice

et al. 2001

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“…27 (Thermo Spectronic, Rochester, NY), as in our previous study. 15,24 The other cationic lipids, Lipofectamine (DOSPA/DOPE), Lipofectamine 2000 (LA2000), DMRIE-C, Cellfectin (TMTPS/DOPE), Lipofectin (DOTMA/DOPE) were purchased from Invitrogen and FuGENE6 was purchased from Roche (Indianapolis, IN).…”

Section: In Vitro Invasion Assaysmentioning

confidence: 99%

“…15 Briefly, NUGC-4 cells (1.5 3 10 4 well 21 ) were precultured in 96-well plates for 24 hr, and medium was removed. The various cationic lipid/pCMV-SPORTb-gal complexes or the cationic lipid/pCMV-SPORTb-gal/HMG-1, 2 complexes were employed in each well.…”

Section: In Vitro Onpg Assaysmentioning

confidence: 99%

“…In our study, tailor-made approaches were made both for the selection of the gefitinib-sensitive SGC cell lines and the design of the NK4-expressing ternary complex. We examined the potential of a specifically designed cationic lipid composition with a particular lipid/DNA ratio modified with a high mobility group-1, 2 chromatin protein (HMG-1, 2), which possesses an introduced DNA stabilizer and a transcriptional activator [15][16][17] for a highly gefitinibsensitive SGC cell line. This tailor-made combination may effectively inhibit the progression of peritoneal SGC dissemination.…”

mentioning

confidence: 99%

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Preclinical study of a “tailor-made” combination of NK4-expressing gene therapy and gefitinib (ZD1839, Iressa™) for disseminated peritoneal scirrhous gastric cancer

Namiki¹,

Namiki²,

Yoshida³

et al. 2005

Int. J. Cancer

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We evaluated the effect of a ''tailor-made'' chemo-gene therapy in scirrhous gastric cancer (SGC)-bearing nude mice. For this tailormade approach, we first selected gefitinib (epidermal growth factor receptor-tyrosine kinase inhibitor)-sensitive SGC cell lines, and 5/8 cell lines demonstrated various degrees of gefitinib-sensitivity. In the highly gefitinib-sensitive NUGC-4, the biological response to NK4 (HGF antagonist/angiogenesis inhibitor) was examined. Subsequently, the composition of an NK4-expressing ternary complex (cationic lipid/nucleic acid/HMG-1, 2 protein) was optimized for maximum transfection activity in NUGC-4. Finally, mice were peritoneally coinoculated with NUGC-4 and scirrhous-associated gastric fibroblasts, NF22, on day 0. Animal models were orally administrated gefitinib (50 mg/kg/day, on days 7-28), and peritoneally NK4-expressing ternary complex (on days 14, 21 and 28). NK4-expression suppressed the gefitinib-resistance induced by the interaction between fibroblasts and SGC, and eventually, this tailor-made combination synergistically decelerated the disease progression by inhibiting proliferative, angiogenic and antiapoptotic effects in tumor tissues. On day 28, both the hemoglobin concentration (g/dl) (control (n 5 8), 11.9; treated (n 5 8), 17.3; p 5 0.0014) and the numbers of mice in good condition (control, 2; treated, 8; p 5 0.0012) were significantly greater, and the abdominal girth (mm) (control, 81.1; treated, 70.3; p 5 0.0036) was significantly reduced. The median points of bloody ascite-free survival time (days) (control, 22; treated, 44; p < 0.0001) and time to euthanasia (days) (control, 36.5; treated, 56; p < 0.0001) were also significantly prolonged. This combination is a potentially useful approach to the treatment of peritoneal gefitinib-sensitive SGC dissemination. ' 2005 Wiley-Liss, Inc.

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“…110,111 To facilitate cytoplasmic transit to the cell nucleus, high mobility group proteins with nuclear localization signals have been complexed to the DNA. 112,113 Most recently, elements from Epstein-Barr virus (oriP and EBNA-1) have been included in the DNA within liposomes to prolong the retention of transgenes in dividing cells. 114 Few studies have used naked DNA and cationic liposomes to transfer genes into cells of the rodent brain.…”

Section: Lentivirus Vectorsmentioning

confidence: 99%

Gene therapy in the CNS

et al. 2000

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Gene transduction for disseminated intraperitoneal tumor using cationic liposomes containing non-histone chromatin proteins: cationic liposomal gene therapy of carcinomatosa

Cited by 44 publications

References 23 publications

Anti-metastatic gene therapy utilizing subcutaneous inoculation of EC-SOD gene transduced autologous fibroblast suppressed lung metastasis of Meth-A cells and 3LL cells in mice

Anti-metastatic gene therapy utilizing subcutaneous inoculation of EC-SOD gene transduced autologous fibroblast suppressed lung metastasis of Meth-A cells and 3LL cells in mice

Preclinical study of a “tailor-made” combination of NK4-expressing gene therapy and gefitinib (ZD1839, Iressa™) for disseminated peritoneal scirrhous gastric cancer

Gene therapy in the CNS

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