Gene transcription profiling in pollutant exposed mussels (Mytilus spp.) using a new low-density oligonucleotide microarray

Dondero, Francesco; Piacentini, Luciana; Marsano, Francesco; Rebelo, Mauro de Freitas; Vergani, Laura; Venier, Paola; Viarengo, Aldo

doi:10.1016/j.gene.2006.02.015

Cited by 102 publications

(42 citation statements)

References 58 publications

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“…Likewise, in the oyster Crassostrea virginica, a strong overexpression of the gene was seen in the hepatopancreas and gills when they were exposed to high Cd concentrations and temperature (Ivanina et al 2009). The increased expression of the mRNA of HSP70 in bivalves is also associated with the response to stress after exposure to metals and xenobiotics (Franzellitti and Fabri 2005;Dondero et al 2006;Song et al 2006).…”

Section: Relative Gene Expressionmentioning

confidence: 99%

Trace metal and biomarker levels in tissues ofArgopecten purpuratusin the north of Chile, and the potential use of this species as a bioindicator of metallic stress

Zapata

Lang

Riso

et al. 2012

Aquat. Living Resour.

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Trace metal and biomarker levels in tissues of Argopecten purpuratus in the north of Chile, and the potential use of this species as a bioindicator of metallic stress Abstract -The capacity to bioaccumulate trace metals present in San Jorge Bay, Antofagasta, Chile, was determined in northern scallop, Argopecten purpuratus, to examine the value of this important commercially species as a bioindicator of heavy metal pollution in areas where it is cultured. Scallops were sampled in summer 2009 in four sites: three natural populations (Coloso, Historic District and La Rinconada, marine reserve), and a commercial hatchery (Colorado). The concentrations of three heavy metals (copper, cadmium and lead) were then determined through stripping chronopotentiometric methods, and the levels of four biomarkers: three genes implicated in the stress and oxidative metabolism, i.e., glutathione peroxidase (GPx), glutathione s-transferase (GST) and heat shock protein 70 (HSP70), and a protein marker in the digestive gland and gill, a metallothionein (MT). The Historic District, located in the downtown area of the city, showed the highest metal concentration of all the sampled sites, as well as the highest levels of the four biomarkers. This can be largely attributed to the nearby international port area of the town and high sea traffic flow, exacerbated by the prevailing winds.

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Section: Relative Gene Expressionmentioning

confidence: 99%

Trace metal and biomarker levels in tissues ofArgopecten purpuratusin the north of Chile, and the potential use of this species as a bioindicator of metallic stress

Zapata

Lang

Riso

et al. 2012

Aquat. Living Resour.

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“…In the blue mussel, a species commonly used as a sentinel to monitor pollution in the marine environment, a first cDNA microarray was designed including 1,714 probes allowing the identification of about 50 signatures of relevant doses of pollutants in mussel tissues (Venier et al 2006). A low-density oligonucleotide microarray, representing 24 mussel genes selected on the basis of their potential involvement in mechanisms of pollutants and xenobiotic response, was also validated (Dondero et al 2006). Finally, from microarray data obtained in the intertidal mussel Mytilus californiacus across major portions of its biogeographical range, Place et al (2008) emphasised the usefulness of such transcriptomic tools to marine ecologists for ecological studies including those relevant to the marine estuarine habitat of the bivalves.…”

Section: Functional Genomic Toolsmentioning

confidence: 99%

Genomic Approaches in Aquaculture and Fisheries

Cancela

Bargelloni

Boudry

et al. 2010

Introduction to Marine Genomics

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Despite the enormous input into the worldwide development of fish and shellfish farming in the recent decades, in part as an attempt to minimize the impact of fishing on already overexploited natural populations, the application of genomics to aquaculture and fisheries remains poorly developed. Improving state-of-the-art genomics research in various aquaculture systems, as well as its industrial applications, remains one of the major challenges in this area and should be the focus of well developed strategies to be implemented in the next generation of projects. This chapter will first provide an overview of the genomic tools and resources available, then discuss the application of genomic approaches to the improvement of fish and shellfish farming (e.g. breeding, reproduction, growth, nutrition and product quality), including the evaluation of stock diversity and the use of selection procedures. The chapter will also discuss the use of genomic approaches to study and monitor natural fish and shellfish populations and to understand interactions within their ecosystems.

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“…An early EST project using multiple tissues from unstressed blue mussels revealed an expression profile and sequence data of known and unknown genes (Venier et al 2003), and this information was recently used to design a lowdensity DNA oligo array for stress response detection (Dondero et al 2006). Such small arrays may advance the use of genomics in marine biomonitoring.…”

Section: Applicationsmentioning

confidence: 99%

Marine ecological genomics: when genomics meets marine ecology

Dupont¹,

Wilson²,

Obst³

et al. 2007

Mar. Ecol. Prog. Ser.

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Genomics, proteomics and metabolomics (the 'omic' technologies) have revolutionized the way we work and are able to think about working, and have opened up hitherto unimagined opportunities in all research fields. In marine ecology, while 'standard' molecular and genetic approaches are well known, the newer technologies are taking longer to make an impact. In this review we explore the potential and promise offered by genomics, genome technologies, expressed sequence tag (EST) collections, microarrays, proteomics and bar coding for modern marine ecology. Methods are succinctly presented with both benefits and limitations discussed. Through examples from the literature, we show how these tools can be used to answer fundamental ecological questions, e.g. 'what is the relationship between community structure and ecological function in ecosystems?'; 'how can a species and the phylogenetic relationship between taxa be identified?'; 'what are the factors responsible for the limits of the ecological niche?'; or 'what explains the variations in life-history patterns among species?' The impact of ecological ideas and concepts on genomic science is also discussed.

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Gene transcription profiling in pollutant exposed mussels (Mytilus spp.) using a new low-density oligonucleotide microarray

Cited by 102 publications

References 58 publications

Trace metal and biomarker levels in tissues ofArgopecten purpuratusin the north of Chile, and the potential use of this species as a bioindicator of metallic stress

Trace metal and biomarker levels in tissues ofArgopecten purpuratusin the north of Chile, and the potential use of this species as a bioindicator of metallic stress

Genomic Approaches in Aquaculture and Fisheries

Marine ecological genomics: when genomics meets marine ecology

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