Gene Transcription Profile in Mice Vaccinated with Ultraviolet-attenuated Cercariae of &amp;lt;italic&amp;gt;Schistosoma japonicum&amp;lt;/italic&amp;gt; Reveals Molecules Contributing to Elevated IFN-&amp;gamma; Levels

Zhu, Xin‐Guang; Zhang, Zhao-Song; Ji, Minjun; Wu, Haiwei; Wang, Yong; Cai, Xiaolin; Zhang, Lei; Hu, Shuying; Fu, Lei; Li, Feng; Su, Chuan; Wu, Guan-Ling

doi:10.1111/j.1745-7270.2005.00038.x

Cited by 4 publications

(8 citation statements)

References 43 publications

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“…This could explain why Th1 immune response with increased IFN-γ predominates in early infection and Th2 type predominates as egg production and tissue reaction begins. Furthermore, the decrease in INF-γ as infection progresses is accompanied by an increase in granuloma formation [48]. Thus, an enhanced Th2 cytokine synthesis was observed during the formation of granulomas in infected mice [49,50] or in animals injected with schistosome eggs [51,52].…”

Section: Discussionmentioning

confidence: 99%

Using poly-N-acetyl glucosamine gel matrix to deliver IL-12 with anti-schistosomasis vaccination

Salem

Attia

Al-Bolkiny

et al. 2010

J Infect Dev Ctries

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Background: Interleukin (IL)-12 is a potential adjuvant in a variety of diseases including schistosomiasis. The clinical use of IL-12, however, is limited by the toxicity associated with its systemic administration. We have developed a novel delivery system (designated F2 gel matrix) composed of poly-N-acetyl glucosamine that has the dual properties of sustaining the release of proteins (e.g. interleukins) and adjuvant effects. The main aim of this study was to use a mouse model to test whether IL-12 released from F2 gel can induce adjuvant effects in the schistosomiasis setting as compared to those obtained after systemic delivery of IL-12. Methodology: First, we compared the toxicity induced by paracrine (delivered by F2 gel) and systemic IL-12. Second, we compared the induction of cytokines induced by paracrine and systemic IL-12. Third, we compared the adjuvant effects of paracrine and systemic IL-12-based prophylactic vaccination against schistosomiasis using soluble worm antigen preparation (SWAP). Results: IL-12 released from F2 gel did not induce significant toxicity measured by alanine aminotransferase (ALT). We found similar serum levels of IFN-γ, TNF-α and IL-2 after paracrine and systemic IL-12 treatments. We also found that vaccination with F2 gel/SWAP/IL-12 induced higher anti-schistosomal effects than IL-12/SWAP as evidenced by 1) the decrease in the total liver egg counts; 2) the reduction in the granuloma size and fibrotic reaction in the liver; and 3) the amelioration of the liver functions. Conclusion: Collectively, these results indicate that IL-12-F2 gel delivery approach could be considered as a potential strategy for the treatment of schistosomiasis.

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Section: Discussionmentioning

confidence: 99%

Using poly-N-acetyl glucosamine gel matrix to deliver IL-12 with anti-schistosomasis vaccination

Salem

Attia

Al-Bolkiny

et al. 2010

J Infect Dev Ctries

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“…It is well-known that IFN-γ acts as a potent activator of macrophages and plays a pro-inflammatory role, exerting key effects in antagonzing pathogen infection. Interleukin (IL) -12 and IL-4, the potent inducers of Th1 and Th2 responses, respectively, as well as IL-10, showed no differences between the infected and vaccinated mice[4]. These observations imply that IFN-γ might represent a key cytokine in regulating the different immune responses induced by normal and radiated cercaria.…”

Section: Introductionmentioning

confidence: 94%

“…UV-radiation-attenuated S. japonicum cercariae elicited predominantly a Th1 response in mice at the early stage of the infection, whereas normal cercariae stimulated primarily Th2-dependent responses[4]. Interferon-γ (IFN-γ) levels were significantly higher in vaccinated mice than in infected mice in the sdLNs in the early stage of the infection[4]. It is well-known that IFN-γ acts as a potent activator of macrophages and plays a pro-inflammatory role, exerting key effects in antagonzing pathogen infection.…”

Section: Introductionmentioning

confidence: 96%

“…Comparative soluble proteomic analysis of normal cercariae (NC) and ultraviolet (UV) -radiationattenuated cercariae (UVAC) from Schistosoma japonicum ( S. japonicum ) revealed that some proteins showed significant differential expression in the parasite after treatment with ultraviolet light[3]. It also has been proved that the inoculation of radiation-attenuated S. japonicum cercaria induce different immune responses from those of a normal cercaria infection[4], both in skin and the local skin-draining lymph nodes (sdLNs) and in spleen. UV-radiation-attenuated S. japonicum cercariae elicited predominantly a Th1 response in mice at the early stage of the infection, whereas normal cercariae stimulated primarily Th2-dependent responses[4].…”

Section: Introductionmentioning

confidence: 99%

“…It also has been proved that the inoculation of radiation-attenuated S. japonicum cercaria induce different immune responses from those of a normal cercaria infection[4], both in skin and the local skin-draining lymph nodes (sdLNs) and in spleen. UV-radiation-attenuated S. japonicum cercariae elicited predominantly a Th1 response in mice at the early stage of the infection, whereas normal cercariae stimulated primarily Th2-dependent responses[4]. Interferon-γ (IFN-γ) levels were significantly higher in vaccinated mice than in infected mice in the sdLNs in the early stage of the infection[4].…”

Section: Introductionmentioning

confidence: 99%

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Antigen presenting cells may be able to distinguish between normal and radiated Schistosoma japonicum cercaria: an in vitro observation

Tang

et al. 2010

Journal of Biomedical Research

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ObjectiveTo observe the discrepancies of responses induced by Schistosoma japonicum (S. japonicum) normal cercaria antigen (NCA) and ultraviolet (UV) -radiation-attenuated cercaria antigen (UVACA) in an in vitro system.MethodsS. japonicum cercariae were collected and UVACA and NCA were prepared. Mouse macrophage model cells (RAW 264.7) were treated with medium, NCA (40 µg/mL) or UVACA (40 µg/mL) in the presence or absence of recombinant mouse interferon gamma (rmIFN-γ; 4 ng/mL) for 48 h. Cell surface staining and flow cytometry were used to assess the major histocompatibility complex (MHC)γ; 4 ng/mL) for 48 h. Cell surface staining and flow cytometry were used to assess the major histocompatibility complex (MHC) II expression, and data were expressed as mean fluorescence intensities (MFI). Interleukin (IL) -10, IL-6 and prostaglandin E2 (PGE2) in cell culture supernatant were evaluated by commercial enzyme-linked immunosorbent assays.ResultsNCA significantly suppressed IFN-γ-induced MHC II expression on RAW 264.7 cells. In the presence of IFN-γ, NCA significantly promoted IL-6, IL-10 and PGE2 secretion from RAW 264.7 cells. In the presence of IFN-γ, UVACA significantly promoted IL-10 but not IL-6 and PGE2 secretion from RAW 264.7 cells and showed no effect on IFN-γ-induced MHC II expression. Compared with UVACA, NCA significantly suppressed IFN-γ-induced MHC II expression and significantly promoted IL-6, PGE2 and IL-10 secretion from RAW 264.7 cells.ConclusionRAW 264.7 cells respond differently to NCA and UVACA. NCA can significantly suppress IFN-γ-induced MHC II expression and significantly promote IL-6, IL-10 and PGE2 secretion from RAW 264.7 cells compared with UVACA.

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Immunomodulatory effects of IL-12 released from poly-N-acetyl glucosamine gel matrix during schistosomiasis infection

et al. 2013

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We have reported recently that Interleukin-12 (IL-12) released from poly-N-acetyl glucosamine gel matrix (F2 gel/IL-12) is more effective than free IL-12 to enhance vaccination of mice with Schistosoma soluble worm antigen preparation. The aim of this study is to evaluate the effect of F2 gel/IL-12 on the inflammatory responses in mice undergoing schistosomiasis infection in absence of vaccination. To achieve this, mice undergoing Schistosoma mansoni infection or cured from this infection, after treatment with praziquantil (PZQ), were treated with subcutaneous injection of IL-12 for 3 consecutive days or once with F2 gel loaded with IL-12 (F2 gel/IL-12). The treatment was started on day 35 days after infection. For infection, mice were infected with 100 cercariae of S. mansoni using tail immersion method. We found that treatment with F2 gel/IL-12 induced significant decreases in the egg burden with a moderate reduction in the size of granuloma and decrease in the cellular granulomatous reaction in the lung as compared to infected mice treated with IL-12. These effects of F2 gel/IL-12 were more pronounced in infected mice previously treated with the anti-schistosomal drug PZQ. The total numbers of white blood cells in all treated mice showed similar profile. Treatment with IL-12 or F2 gel/IL-12, however, showed significant reduction in the number of mononuclear cells when compared with non-treated infected mice. In conclusion, this study showed the ability of IL-12 released from F2 gel to lower the inflammatory response to Schistosoma infection even in absence of vaccination.

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Gene Transcription Profile in Mice Vaccinated with Ultraviolet-attenuated Cercariae of <italic>Schistosoma japonicum</italic> Reveals Molecules Contributing to Elevated IFN-γ Levels

Cited by 4 publications

References 43 publications

Using poly-N-acetyl glucosamine gel matrix to deliver IL-12 with anti-schistosomasis vaccination

Using poly-N-acetyl glucosamine gel matrix to deliver IL-12 with anti-schistosomasis vaccination

Antigen presenting cells may be able to distinguish between normal and radiated Schistosoma japonicum cercaria: an in vitro observation

Immunomodulatory effects of IL-12 released from poly-N-acetyl glucosamine gel matrix during schistosomiasis infection

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