Gene transcription from the linear plasmid pBClin15 leads to cell lysis and extracellular DNA-dependent aggregation of Bacillus cereus ATCC 14579 in response to quinolone-induced stress

Vörös, Aniko; Simm, Roger; Kroeger, Jasmin K.; Kolstø, Anne‐Brit

doi:10.1099/mic.0.069674-0

Cited by 5 publications

(6 citation statements)

References 45 publications

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“…This helps the bacteria to cope with adverse environments [21]. This may also increase genetic stability by limiting high-frequency mutations, based on the accumulation of DNA damage.…”

Section: Resultsmentioning

confidence: 99%

“…Growth characteristics of the B. cereus ATCC 14579 and ΔpBClin15 (pBClin15-cured) strains A pBClin15-cured B. cereus ATCC 14579 strain, named ΔpBClin15, was constructed as described by Voros et al [21]. The removal of pBClin15 was confirmed by PCR.…”

Section: Resultsmentioning

confidence: 99%

“…This strain was cured of pBClin15 as described by Voros et al [21], resulting in the ΔpBClin15 strain. B. cereus strains (with and without pBClin15) were cultured in batches (three independent cultivations per strain) at pO 2 = 100% [22] in minimal MOD medium supplemented with 30 mM glucose as the carbon source [23].…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

“…With the emergence of "global systems biology" tools (mainly DNA microarrays), cryptic prophages have gradually gained attention, as part of host physiology, especially under stress conditions [21]. Our goal here was to understand the importance of the effect of pBClin15 on B. cereus physiology under normal growth conditions.…”

Section: Introductionmentioning

confidence: 99%

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Deciphering the interactions between the Bacillus cereus linear plasmid, pBClin15, and its host by high-throughput comparative proteomics

Madeira

Omer

Alpha-Bazin

et al. 2016

Journal of Proteomics

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Bacteria belonging to the Bacillus cereus group include B. cereus, a notorious food borne pathogen which causes gastroenteritis. The B. cereus type, strain ATCC 14579, harbors a linear plasmid, pBClin15, which displays cryptic prophage behavior. Here, we present data supporting the idea that pBClin15 may have a much greater role in B. cereus metabolism that has hitherto been suspected. Specifically, our comparative proteomic analyses reveal that pBClin15 manages B. cereus central metabolism to optimize energy and carbon utilization through the repression of several chromosome-encoded phage proteins. These results suggest that pBClin15 provides benefit to the host for surviving adverse environmental conditions.

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“…This helps the bacteria to cope with adverse environments [21]. This may also increase genetic stability by limiting high-frequency mutations, based on the accumulation of DNA damage.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Deciphering the interactions between the Bacillus cereus linear plasmid, pBClin15, and its host by high-throughput comparative proteomics

Madeira

Omer

Alpha-Bazin

et al. 2016

Journal of Proteomics

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“…Likewise, no noteworthy differences in the antibiograms displayed by the nonlysogenic strain and the two lysogens were found. Interestingly, the B. cereus ATCC 14579 strain cured of plasmid pBClin15 was more tolerant to antibiotics interfering with DNA integrity (i.e., Nal, norfloxacin, and ciprofloxacin) than the wild-type strain (31). Since the nonlysogenic strain GBJ002 is Nal r , the influence of tectiviral lysogeny on the quinolone response might be masked by the chromosome-encoded antibiotic resistance.…”

Section: Figmentioning

confidence: 99%

Influence of Lysogeny of Tectiviruses GIL01 and GIL16 on Bacillus thuringiensis Growth, Biofilm Formation, and Swarming Motility

Gillis

Mahillon

2014

Appl Environ Microbiol

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Bacillus thuringiensis is an entomopathogenic bacterium that has been used as an efficient biopesticide worldwide. Despite the fact that this bacterium is usually described as an insect pathogen, its life cycle in the environment is still largely unknown. B. thuringiensis belongs to the Bacillus cereus group of bacteria, which has been associated with many mobile genetic elements, such as species-specific temperate or virulent bacteriophages (phages). Temperate (lysogenic) phages are able to establish a longterm relationship with their host, providing, in some cases, novel ecological traits to the bacterial lysogens. Therefore, this work focuses on evaluating the potential influence of temperate tectiviruses GIL01 and GIL16 on the development of different life traits of B. thuringiensis. For this purpose, a B. thuringiensis serovar israelensis plasmid-cured (nonlysogenic) strain was used to establish bacterial lysogens for phages GIL01 and GIL16, and, subsequently, the following life traits were compared among the strains: kinetics of growth, metabolic profiles, antibiotics susceptibility, biofilm formation, swarming motility, and sporulation. The results revealed that GIL01 and GIL16 lysogeny has a significant influence on the bacterial growth, sporulation rate, biofilm formation, and swarming motility of B. thuringiensis. No changes in metabolic profiles or antibiotic susceptibilities were detected. These findings provide evidence that tectiviruses have a putative role in the B. thuringiensis life cycle as adapters of life traits with ecological advantages.

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SecDF as Part of the Sec-Translocase Facilitates Efficient Secretion of Bacillus cereus Toxins and Cell Wall-Associated Proteins

et al. 2014

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The aim of this study was to explore the role of SecDF in protein secretion in Bacillus cereus ATCC 14579 by in-depth characterization of a markerless secDF knock out mutant. Deletion of secDF resulted in pleiotropic effects characterized by a moderately slower growth rate, aberrant cell morphology, enhanced susceptibility to xenobiotics, reduced virulence and motility. Most toxins, including food poisoning-associated enterotoxins Nhe, Hbl, and cytotoxin K, as well as phospholipase C were less abundant in the secretome of the ΔsecDF mutant as determined by label-free mass spectrometry. Global transcriptome studies revealed profound transcriptional changes upon deletion of secDF indicating cell envelope stress. Interestingly, the addition of glucose enhanced the described phenotypes. This study shows that SecDF is an important part of the Sec-translocase mediating efficient secretion of virulence factors in the Gram-positive opportunistic pathogen B. cereus, and further supports the notion that B. cereus enterotoxins are secreted by the Sec-system.

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Gene transcription from the linear plasmid pBClin15 leads to cell lysis and extracellular DNA-dependent aggregation of Bacillus cereus ATCC 14579 in response to quinolone-induced stress

Cited by 5 publications

References 45 publications

Deciphering the interactions between the Bacillus cereus linear plasmid, pBClin15, and its host by high-throughput comparative proteomics

Deciphering the interactions between the Bacillus cereus linear plasmid, pBClin15, and its host by high-throughput comparative proteomics

Influence of Lysogeny of Tectiviruses GIL01 and GIL16 on Bacillus thuringiensis Growth, Biofilm Formation, and Swarming Motility

SecDF as Part of the Sec-Translocase Facilitates Efficient Secretion of Bacillus cereus Toxins and Cell Wall-Associated Proteins

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